Abstract: Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the 10 transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (1) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Abstract: Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (l) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Abstract: A method and an apparatus for acquiring induced polarization parameters by using a spread spectrum signal, a medium and a device are provided, where the method includes: sending a spread spectrum signal to a detection object and acquiring time sequence data of the spread spectrum signal; acquiring a frequency spectrum value of each main frequency in the spread spectrum signal, and obtaining a normalized complex value of each main frequency based on the frequency spectrum value of each main frequency; obtaining a frequency value of each combined frequency in the spread spectrum signal; calculating a normalized complex value of each combined frequency according to the normalized complex value of each main frequency; and calculating induced polarization parameters of all combined frequencies according to frequency values of all combined frequencies and normalized complex values of all combined frequencies.

Abstract: Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Abstract: Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Abstract: Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (l) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Abstract: Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (1) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Abstract: Provided is a combined suction anchor reinforced by a grouting spiral anchor, including a gravity suction anchor, an L-shaped skirt board and a spiral anchor. The gravity suction anchor includes a suction anchor top plate and a wall of a suction anchor barrel body and uses a concrete press block to reach an estimated set position faster during penetration due to self-weight thereof. Thereafter, the skirt board is lifted to outside of the suction anchor through a lug, and a fixing bolt is used to connect the skirt board to the suction anchor. The spiral anchor rod passes through a skirt board hole performed in the skirt board to penetrate into a deep site of seabed, and concrete is grouted through the skirt board hole to a seabed soil area loosened due to penetration of the spiral anchor rod, thereby forming a concrete reinforced area after the concrete solidifies.

Abstract: A method of processing a workpiece may include forming a first layer on a first side of a base layer. The base layer may be part of a substrate including a plurality of layers. The method may also include forming a second layer on the first layer. A material of the second layer may include metal. The method may also include forming an opening in the second layer, forming an opening in the first layer by etching, and removing the second layer. The method may include dry etching of the first layer.

Abstract: The present invention provides a terminal control method, a protective case, and a terminal. In the method, a terminal may be disposed with two Hall effect sensors for sensing a change of a magnetic field generated by a magnet in a protective case, and identify a forward snap-fit operation, an opening operation, a backward snap-fit operation, and the like of the protective case. When detecting the forward snap-fit operation of the protective case, the terminal performs a screen off operation; or when detecting the backward snap-fit operation of the protective case, the terminal may skip performing screen off operation. In embodiments of the present invention, the protective case can be prevented from turning off a screen by mistake, so as to improve terminal control accuracy.

Abstract: A display panel, a display device, and a method of manufacturing a display panel are provided. As an example, the display panel includes an array layer, a planarization layer, an inorganic layer, and at least one dam. The array layer includes a first region and a second region surrounding the first region. The planarization layer is at least partially located on a portion of the array layer which is at the first region. The inorganic layer is located on a portion of the array layer which is at the second region. The at least one dam is formed on the inorganic layer.

Abstract: Provided is a combined suction anchor reinforced by a grouting spiral anchor, including a gravity suction anchor, an L-shaped skirt board and a spiral anchor. The gravity suction anchor includes a suction anchor top plate and a wall of a suction anchor barrel body and uses a concrete press block to reach an estimated set position faster during penetration due to self-weight thereof. Thereafter, the skirt board is lifted to outside of the suction anchor through a lug, and a fixing bolt is used to connect the skirt board to the suction anchor. The spiral anchor rod passes through a skirt board hole performed in the skirt board to penetrate into a deep site of seabed, and concrete is grouted through the skirt board hole to a seabed soil area loosened due to penetration of the spiral anchor rod, thereby forming a concrete reinforced area after the concrete solidifies.

Abstract: The present disclosure discloses a testing apparatus for directional simulation of dynamic collision between a deep-sea shell structure and seabed, including: a launching device, a high-pressure water pump device, a high-speed camera, a sensor system, a data collection and control system, etc. This device is installed in a geotechnical centrifuge for experiment, a super-gravity environment is provided to meet requirements of simulation of a deep-sea environment, and a deep-sea high-pressure environment is created through a high-pressure water pump device by superposition. A direction of the launching device is adjusted through a universal rotating shaft to control the shell structure to be launched from a specified direction to collide with soil at a predetermined position. A high-speed camera is used to capture an entire experiment process, and strain and acceleration sensors are used to collect experiment data.

Abstract: The present disclosure discloses a testing apparatus for directional simulation of dynamic collision between a deep-sea shell structure and seabed, including: a launching device, a high-pressure water pump device, a high-speed camera, a sensor system, a data collection and control system, etc. This device is installed in a geotechnical centrifuge for experiment, a super-gravity environment is provided to meet requirements of simulation of a deep-sea environment, and a deep-sea high-pressure environment is created through a high-pressure water pump device by superposition. A direction of the launching device is adjusted through a universal rotating shaft to control the shell structure to be launched from a specified direction to collide with soil at a predetermined position. A high-speed camera is used to capture an entire experiment process, and strain and acceleration sensors are used to collect experiment data.

Abstract: Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (1) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Abstract: The present invention provides a terminal control method, a protective case, and a terminal. In the method, a terminal may be disposed with two Hall effect sensors for sensing a change of a magnetic field generated by a magnet in a protective case, and identify a forward snap-fit operation, an opening operation, a backward snap-fit operation, and the like of the protective case. When detecting the forward snap-fit operation of the protective case, the terminal performs a screen off operation; or when detecting the backward snap-fit operation of the protective case, the terminal may skip performing screen off operation. In embodiments of the present invention, the protective case can be prevented from turning off a screen by mistake, so as to improve terminal control accuracy.

Abstract: Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Abstract: In a terminal control method, a terminal may be disposed with two Hall effect sensors for sensing a change of a magnetic field generated by a magnet in a protective case, and identify a forward snap-fit operation, an opening operation, a backward snap-fit operation, and the like of the protective case. When detecting the forward snap-fit operation of the protective case, the terminal performs a screen off operation; or when detecting the backward snap-fit operation of the protective case, the terminal may skip performing screen off operation. Thus, the protective case can be prevented from turning off a screen by mistake.

Abstract: Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Abstract: A display panel, a display device, and a method of manufacturing a display panel are provided. As an example, the display panel includes an array layer, a planarization layer, an inorganic layer, and at least one dam. The array layer includes a first region and a second region surrounding the first region. The planarization layer is at least partially located on a portion of the array layer which is at the first region. The inorganic layer is located on a portion of the array layer which is at the second region. The at least one dam is formed on the inorganic layer.