Abstract: A microbial preparation and a method for remedying a polycyclic aromatic hydrocarbons (PAHs)-contaminated soil are disclosed. The microbial preparation contains Pseudomonas sp. KW-2 with a deposit number of CGMCC No. 29017. In the soil remediation method, an oxidizing agent is added to the PAHs-contaminated soil for pre-oxidation treatment, where the oxidizing agent is selected from the group consisting of Fenton's reagent, hydrogen peroxide, sodium persulfate, potassium permanganate, and a combination thereof; a carbon source or a nitrogen source added to reach a carbon-to-nitrogen ratio of 100:5-20, and the microbial preparation is introduced for microbial remediation, where the carbon source is selected from the group consisting of glucose, fructose, sucrose, starch, and a combination thereof; and the nitrogen source is selected from the group consisting of ammonium chloride, ammonium nitrate, potassium nitrate, sodium nitrate, and a combination thereof.