Abstract: Methods, systems, and computer-readable storage media for receiving, by an operation guard system executed within a cloud platform, session information representative of a session of a user within the cloud platform, the session information including user information and operation information, determining, by the operation guard system, that the user is signed into a technical group for execution of an operation represented in the operation information, and in response, providing, by the operation guard system, a risk score associated with the operation, and determining, by the operation guard system and at least partially based on the risk score, that the operation is a risk-oriented operation based on the risk score, and in response, preventing execution of the operation and transmitting an alert.

Abstract: Various examples are directed to systems and methods for operating a database management system (DBMS) in a cloud environment. An assembly worker executing at a first computing device may provide a first database protocol message generated by a process code executing at the first computing device. A translation engine executed at the cloud environment may translate the first database protocol message from a first format to a second format associated with a DBMS instance executing at the cloud environment to generate a translated first database protocol message. The translation engine may cause the translated first database protocol message to be provided to the DBMS instance and may receive, from the DBMS instance, a first reply corresponding to the translated first database protocol message. The first reply may be sent to the process code.

Abstract: There provided, inter alia, reagents and methods for modulation fluorescence of a tetrazine containing compound and/or a dienophile-containing compound. There is provided a method of detecting binding of a first affmity ligand and a second affinity ligand, the method includes the follow steps. Contacting a tetrazine-containing compound with a dienophile-containing compound, the tetrazine-containing compound comprising a first affinity ligand covalently attached to a tetrazine moiety and the dienophile-containing comprising a second affmity ligand covalently attached to a dienophile moiety.

Abstract: Described herein are tetrazine derivatives and efficient synthetic methods of synthesis thereof using elimination-Heck cascade reaction. Provided herein is the synthesis of conjugated tetrazines from the tetrazine derivatives. Also provided herein are methods of use of the conjugated tetrazines as fluorogenic probes for live-cell imaging.