Abstract: The invention relates to ester compounds of the general formula (I) to a process for preparation thereof and to the use thereof. These ester compounds may contain a mixture of at least two compounds of the general formula (I).

Abstract: A method for producing a hydroxy fatty acid condensate or a mixture of hydroxy fatty acid condensates is provided. The method involves the steps of providing one or more fatty acids having at least one C?C double bond functionality, biotechnologically adding H2O to at least one C?C double bond functionality of the one or more fatty acids and thus obtaining one or more hydroxy fatty acids, and reacting the one or more hydroxy fatty acids with one or more at least divalent linker groups, thus obtaining a hydroxy fatty acid condensate or a mixture of hydroxy fatty acid condensates. Also provided are a hydroxy fatty acid condensate or a mixture of hydroxy fatty acid condensates obtained by the method, as well as polyurethane, obtained by reacting such a hydroxy fatty acid condensate or mixture of hydroxy fatty acid condensates.

Abstract: The present invention provides a carbonyl reductase having the activity of reducing a carbonyl group-containing compound to convert the compound into an optically active compound, and a production method of an optically active compound using the enzyme. Specifically, a carbonyl reductase having one or more mutations in which the 54th aspartic acid, the 157th methionine, the 170th alanine, the 211th isoleucine, the 214th methionine, and the 249th methionine are each substituted by other specific amino acid in the amino acid sequence shown in SEQ ID NO: 1 or a homologue of the amino acid sequence, and a production method of an optically active compound using the same are provided.

Abstract: The present invention is directed to a process for the enantioselective biocatalytic preparation of 4-substituted 1-aminoindanes of general formula (S)-I: (1) and a process for the preparation of Ozanimod, preferably involving the enantioselective biocatalytic process of preparing (S)-4-substituted 1-aminoindanes of the above general formula, wherein R=—CN and Y=H.

Abstract: The invention relates to ester compounds of the general formula (I) to a process for preparation thereof and to the use thereof. These ester compounds may contain a mixture of at least two compounds of the general formula (I).

Abstract: The invention relates to novel ester compounds of the general formula (I) to a process for preparation thereof and to the use thereof. These ester compounds may contain a mixture of at least two compounds of the general formula (I).

Abstract: The invention relates to novel ester compounds of the general formula (I) to a process for preparation thereof and to the use thereof. These ester compounds may contain a mixture of at least two compounds of the general formula (I).

Abstract: The invention relates to a method for preparing an N-acyl- or N-sulfonyl-?-aminonitrile, comprising the following steps: a) condensation of an N-acyl- or N-sulfonyl-?-aminoaldehyde with hydroxylamine to give an aldoxime, and b) dehydration of the aldoxime obtained in step a) to give an N-acyl- or N-sulfonyl-?-aminonitrile. In an advantageous manner, the absolute configuration can be retained in the conversion to the N-acyl- or N-sulfonyl-?-aminonitrile.

Abstract: A product is enriched by a method for the processing of a reaction solution, in which: a) a pH value of the reaction solution is adjusted to less than 4; wherein said reaction solution comprises a whole-cell catalyst, an aqueous component, and an organic component, wherein the organic component contains a product to be enriched; and b) the reaction solution is filtered in the presence of a filter aid, thereby enriching the product.

Abstract: The present invention relates to a process for preparing, in particular, enantiomerically enriched L-?-amino acids, in particular those of the general formula (I). In this connection, the process according to the invention uses 2-ketocarboxylic acids which are converted into the desired products using a whole-cell catalyst which comprises an amino acid dehydrogenase and a cofactor-regenerating enzyme.

Abstract: The present invention is directed towards an asymmetric enzymic process for preparing optically active organic compounds. The process according to the invention is carried out in what are termed miniemulsions. The invention also relates to an enzymic reaction mixture which exhibits a miniemulsion.

Abstract: A process for the preparation of an enantiomerically enriched amine, is performed by a) cleaving a racemic mixture of a reaction product of i) a chiral amine and ii) an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; or b) reacting an amine with an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; and c) separating the enantiomerically enriched amide from the enantiomerically enriched amine, wherein the acyl donor is a sulphonylacetic acid ester. The process (1) leads to high enantioselectivities, and (2) high reactivities, (3) is based on an acyl donor accessible in a simple manner, (4) is suitable for a large number of very different substrates and/or (5) is suitable for carrying out at high substrate concentrations.

Abstract: The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to nonhuman hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohols which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohols with the formation of aldehydes or ketones.

Abstract: The present application relates to a reaction system in which chemically valuable compounds can be obtained in high enantiomer concentrations with the aid of a coupled enzymatically operating transformation process. The coupled enzymatic reaction system comprises a cofactor-dependent enzymatic transformation of an organic compound and an enzymatic regeneration of the cofactor, wherein the reaction system operates in aqueous solution with an amount of substrate above the solubility limit thereof. In the preferred embodiments, an alcohol dehydrogenase is the cofactor-dependent enzyme, and the regeneration of the cofactor (e.g. NADH or NADPH) is acheved by means of formate dehydrogenase.

Abstract: The present invention relates to a process for the preparation of optically active alcohols from ketones with the aid of whole-cell catalysts comprising an alcohol dehydrogenase and also an enzyme capable of cofactor regeneration, a substrate concentration of at least 500 mM of ketone being provided for the conversion and the conversion being carried out without the addition of an “external” cofactor.

Abstract: A process for preparing enantiomerically enriched amines by reacting a ketone with ammonia or an ammonium salt and a reducing agent in the presence of a catalytic system comprising the components: a) an amino acid transaminase, b) an alpha-amino acid which is a substrate of the amino acid transaminase, c) an amino acid dehydrogenase suitable for preparing the alpha-amino acid, d) NAD(P)+ and e) an NAD(P)+-reducing enzyme, which reacts NAD(P)+ with the reducing agent to give NAD(P)H. The process can be carried out with catalytic amounts of alpha-amino acid and NAD(P)+, and enables an enantioselective reductive amination of ketones.

Abstract: The present invention relates to a process for preparing, in particular, enantiomerically enriched L-?-amino acids, in particular those of the general formula (I). In this connection, the process according to the invention uses 2-ketocarboxylic acids which are converted into the desired products using a whole-cell catalyst which comprises an amino acid dehydrogenase and a cofactor-regenerating enzyme.

Abstract: The present invention relates to an alcohol dehydrogenase from Rhodococcus erythropolis. By means of such cofactor-dependent ADHs, chiral alcohols, which can be of use for use in organic syntheses, can advantageously be obtained with a cofactor-regenerating enzyme in a coupled enzymatic system. A nucleotide sequence, vehicles containing this, a polypeptide sequence and processes for mutation and use of the sequences are claimed.

Abstract: A product is enriched by a method for the processing of a reaction solution, in which: a) a pH value of the reaction solution is adjusted to less than 4; wherein said reaction solution comprises a whole-cell catalyst, an aqueous component, and an organic component, wherein the organic component contains a product to be enriched; and b) the reaction solution is filtered in the presence of a filter aid, thereby enriching the product.

Abstract: A process for the preparation of an enantiomerically enriched amine, is performed by a) cleaving a racemic mixture of a reaction product of i) a chiral amine and ii) an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; or b) reacting an amine with an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; and c) separating the enantiomerically enriched amide from the enantiomerically enriched amine, wherein the acyl donor is a sulphonylacetic acid ester. The process (1) leads to high enantioselectivities, and (2) high reactivities, (3) is based on an acyl donor accessible in a simple manner, (4) is suitable for a large number of very different substrates and/or (5) is suitable for carrying out at high substrate concentrations.