Abstract: Disclosed is an in-process cell monitoring device comprising: a flow channel having at least one inlet and at least one outlet exposeable to a cell culture; a microscope positionable to view the contents of a region of the channel; and a computer operable at least to count any cells in the region, providing a closed fluid circuit for cell monitoring. Disclosed also is a bioreactor including a cell culture volume, and an in-process cell monitoring device, said device comprising: a flow channel having at least one inlet and at least one outlet each in fluid communication with the volume, of sufficient cross-sectional area to allow fluids to drain from the inlet to the outlet; and a microscope positionable to view the contents of a region of the channel, and also A method for monitoring a cell culture including determining cell density.

Abstract: A trocar assembly including a sleeve assembly defining a working channel extending axially therethrough and including an insufflation port in fluid communication with the working channel, and an insufflation valve assembly fluidly coupled to the insufflation port, the insufflation valve assembly including a valve member and a housing, wherein the valve member defines an inlet channel and is biased relative to the housing to a first position in which the inlet channel is in fluid communication with the working channel, and wherein the valve member is moveable relative to the housing from the first position to a second position in which the inlet channel is fluidly decoupled from the working channel.

Abstract: A trocar sleeve assembly including a cannula connected to a housing assembly to define a working channel, wherein the housing assembly includes a housing that defines an opening in fluid communication with the working channel, a sleeve slidably received over the housing to define an annular region between the sleeve and the housing, a first sealing member forming a first seal between the sleeve and the housing, and a second sealing member forming a second seal between the sleeve and the housing, the second sealing member being axially spaced from the first sealing member to define a chamber in the annular region, and an insufflation port in fluid communication with the chamber, wherein the sleeve is slidable relative to the housing between a first position, wherein the chamber is in fluid communication with the opening, and a second position, wherein the chamber is fluidly decoupled from the opening.

Abstract: A trocar sleeve assembly including a cannula connected to a housing assembly to define a working channel, wherein the housing assembly includes a housing that defines an opening in fluid communication with the working channel, a sleeve slidably received over the housing to define an annular region between the sleeve and the housing, a first sealing member forming a first seal between the sleeve and the housing, and a second sealing member forming a second seal between the sleeve and the housing, the second sealing member being axially spaced from the first sealing member to define a chamber in the annular region, and an insufflation port in fluid communication with the chamber, wherein the sleeve is slidable relative to the housing between a first position, wherein the chamber is in fluid communication with the opening, and a second position, wherein the chamber is fluidly decoupled from the opening.

Abstract: A trocar assembly including a sleeve assembly having a cannula connected to a housing and an obturator assembly including a handle connected to an obturator, wherein the obturator assembly is receivable in the sleeve assembly to define a gripping portion that includes the housing and the handle, the gripping portion having an axial length and a maximum width, and wherein a ratio of the axial length to the maximum width ranges from about 2.5 to about 3.5.

Abstract: A trocar assembly including a sleeve assembly defining a working channel extending axially therethrough and including an insufflation port in fluid communication with the working channel, and an insufflation valve assembly fluidly coupled to the insufflation port, the insufflation valve assembly including a valve member and a housing, wherein the valve member defines an inlet channel and is biased relative to the housing to a first position in which the inlet channel is in fluid communication with the working channel, and wherein the valve member is moveable relative to the housing from the first position to a second position in which the inlet channel is fluidly decoupled from the working channel.