Abstract: This invention provides a range of translatable polynucleotide and oligomer molecules for expressing a human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), or a fragment thereof having AGL activity. The polynucleotide and oligomer molecules are expressible to provide the human AGL or a fragment thereof having AGL activity. The molecules can be used as active agents to express an active polypeptide or protein in cells or subjects. The agents can be used in methods for ameliorating, preventing, delaying onset, or treating a disease or condition associated with reduced activity of amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL) in a subject.

Abstract: This invention provides a range of translatable polynucleotide and oligomer molecules for expressing a human phenylalanine hydroxylase (PAH), or a fragment thereof having PAH activity. The polynucleotide and oligomer molecules are expressible to provide the human PAH or a fragment thereof having PAH activity. The molecules can be used as active agents to express an active polypeptide or protein in cells or subjects. The agents can be used in methods for ameliorating, preventing, delaying onset, or treating a disease or condition associated with phenylketonuria, decreased metabolism of phenylalanine, or increased levels of phenylalanine in a subject.

Abstract: The current invention is a method for generating mRNA and purifying it from impure preparations that result from processes used to generate the mRNA. The processes may include the commonly used cell-free, enzymatic synthesis (in-vitro transcription, IVT) or may include preparation of crude cell extracts that contain mRNAs. The key steps in purification involves capture of the mRNA via base-pairing of a covalently attached adapter RNA to the chromatography column, and subsequent on-column cleavage of a substrate-dependent ribozyme, also covalently attached, which cuts and releases the mRNA upon addition of a specific substrate.

Abstract: This invention provides a range of translatable polynucleotide and oligomer molecules for expressing a human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), or a fragment thereof having AGL activity. The polynucleotide and oligomer molecules are expressible to provide the human AGL or a fragment thereof having AGL activity. The molecules can be used as active agents to express an active polypeptide or protein in cells or subjects. The agents can be used in methods for ameliorating, preventing, delaying onset, or treating a disease or condition associated with reduced activity of amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL) in a subject.

Abstract: This invention provides a range of translatable polynucleotide and oligomer molecules for expressing a human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), or a fragment thereof having AGL activity. The polynucleotide and oligomer molecules are expressible to provide the human AGL or a fragment thereof having AGL activity. The molecules can be used as active agents to express an active polypeptide or protein in cells or subjects. The agents can be used in methods for ameliorating, preventing, delaying onset, or treating a disease or condition associated with reduced activity of amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL) in a subject.

Abstract: This invention provides a range of translatable polynucleotide and oligomer molecules for expressing a human phenylalanine hydroxylase (PAH), or a fragment thereof having PAH activity. The polynucleotide and oligomer molecules are expressible to provide the human PAH or a fragment thereof having PAH activity. The molecules can be used as active agents to express an active polypeptide or protein in cells or subjects. The agents can be used in methods for ameliorating, preventing, delaying onset, or treating a disease or condition associated with phenylketonuria, decreased metabolism of phenylalanine, or increased levels of phenylalanine in a subject.

Abstract: A device is provided which enables a courier to select one of two lists and to detect a selection of one of the two lists. A first list is provided for shipments that are to be delivered to a respective destination of the shipments by the courier and a second list is provided for shipments that are not to be delivered to a respective destination of the shipments by the courier. The device receives data that results from a scanning of at least one label that is associated with a shipment or with a group of shipments. The device adds at least one item to the selected list, each of the at least one item associated with a shipment or with a group of shipments, based on the received data. The device may perform these actions by an apparatus and/or by computer program that is executed by a processor.

Abstract: A device is provided which enables a courier to select one of two lists and to detect a selection of one of the two lists. A first list is provided for shipments that are to be delivered to a respective destination of the shipments by the courier and a second list is provided for shipments that are not to be delivered to a respective destination of the shipments by the courier. The device receives data that results from a scanning of at least one label that is associated with a shipment or with a group of shipments. The device adds at least one item to the selected list, each of the at least one item associated with a shipment or with a group of shipments, based on the received data. The device may perform these actions by an apparatus and/or by computer program that is executed by a processor.