Abstract: Various embodiments for systems and methods for producing one-dimensional super-resolved images from diffraction-limited line-confocal images using a trained neral network to generate a one-dimensional super-resolved output as well as an isotropic, in-plane super-resolved image are disclosed, wherein the neural network is trained using a training set comprising a plurality of matched training pairs, each training pair of the plurality of training pairs comprising a diffraction-limited line confocal image of the plurality of diffraction-limited line confocal images of the image type and a one dimensional super resolved image corresponding to the diffraction-limited line confocal image of the plurality of diffraction limited line confocal images.

Abstract: Methods and systems are provided for improving resolution, acquisition speed, and/or illumination dose for microscopy systems. In some embodiments, a microscopy system having multiple objective setups may include illumination generators to provide selectively-blanked illumination line scans, objective lenses to introduce the selectively-blanked illumination line scans to a sample and to collect fluorescence emissions from the sample, and detectors to receive the fluorescence emissions from the objective lenses. The microscopy system may also include one or more processors in operative communication with the detectors, which may combine the fluorescence emissions to generate a composite image.

Abstract: Systems and methods for three-dimensional fluorescence polarization excitation that generates maps of positions and orientation of fluorescent molecules in three or more dimensions are disclosed.

Abstract: Systems and methods for three-dimensional fluorescence polarization excitation that generates maps of positions and orientation of fluorescent molecules in three or more dimensions are disclosed.

Abstract: Systems and methods for three-dimensional fluorescence polarization excitation that generates maps of positions and orientation of fluorescent molecules in three or more dimensions are disclosed.

Abstract: Various embodiments for systems and methods for improved axial resolution in a microscopy using photoswitching and standing-wave illumination techniques are described.

Abstract: A resolution enhancement technique for a line scanning confocal microscopy system that generates vertical and horizontal line scanning patterns onto a sample is disclosed. The line scanning confocal microscopy system is capable of producing line scanning patterns through the use of two alternative pathways that generate either the vertical line scanning pattern or horizontal line scanning pattern.

Abstract: Various embodiments (300, 400, 500) for a multi-focal selective illumination microscopy (SIM) system for generating multi-focal patterns of a sample are disclosed. The embodiments (300, 400, 500) of the multi-focal SIM system perform a focusing, scaling and summing operation on each generated multi-focal pattern in a sequence of multi-focal patterns that completely scan the sample to produce a high resolution composite image.

Abstract: Various embodiments of a multi-photon microscopy system that uses sequential excitation of a sample through three or more objective lenses oriented at different axes intersecting the sample are disclosed. Each objective lens is capable of focused sequential excitation of the sample that elicits fluorescence emissions from the excited sample, which is then simultaneously detected by each respective objective lens along a respective longitudinal axis every time the sample is illuminated through only a single objective lens.

Abstract: Various embodiments for a multi-focal selective illumination microscopy (SIM) system for generating multi-focal patterns of a sample are disclosed. The embodiments of the multi-focal SIM system perform a focusing, scaling and summing operation on each generated multi-focal pattern in a sequence of multi-focal patterns that completely scan the sample to produce a high resolution composite image.

Abstract: Various embodiments related to systems and methods for instant structured microscopy where total internal reflection fluorescence techniques are used to improve optical sectioning and signal-to-noise ratio of structured illumination microscopy are herein disclosed.

Abstract: Systems and methods for three-dimensional fluorescence polarization excitation that generates maps of positions and orientation of fluorescent molecules in three or more dimensions are disclosed.

Abstract: Various embodiments of a multi-photon microscopy system that uses sequential excitation of a sample through three or more objective lenses oriented at different axes intersecting the sample are disclosed. Each objective lens is capable of focused sequential excitation of the sample that elicits fluorescence emissions from the excited sample, which is then simultaneously detected by each respective objective lens along a respective longitudinal axis every time the sample is illuminated through only a single objective lens.

Abstract: A structured illumination microscopy (SIM) system for generating single focal point patterns of a sample is disclosed. The SIM system performs a focusing, scaling and summing operation on each single focal point that completely scan the sample to produce a high resolution composite image.

Abstract: Various embodiments related to systems and methods for instant structured microscopy where total internal reflection fluorescence techniques are used to improve optical sectioning and signal-to-noise ratio of structured illumination microscopy are herein disclosed.

Abstract: Embodiments related to systems and methods for instant structured microscopy where total internal reflection fluorescence techniques are used to improve optical sectioning and signal-to-noise ratio of structured illumination microscopy are disclosed.

Abstract: Various embodiments (300, 400, 500) for a multi-focal selective illumination microscopy (SIM) system for generating multi-focal patterns of a sample are disclosed. The embodiments (300, 400, 500) of the multi-focal SIM system perform a focusing, scaling and summing operation on each generated multi-focal pattern in a sequence of multi-focal patterns that completely scan the sample to produce a high resolution composite image.

Abstract: Embodiments of a resolution enhancement technique for a light sheet microscopy system having a three objective lens arrangement in which one objective lens illuminates a sample and the second and third objective lenses collect the fluorescence emissions emitted by the sample are disclosed. The second objective lens focuses a first portion of the fluorescence emissions for detection by a second detection component, while the third objective lens focuses a second portion of the fluorescence emissions through a diffractive or refractive optic component for detection by a first detector component. A processor combines the images resulting from the first and second portions of the fluorescence emissions for generating composite images with increased axial and lateral resolution.

Abstract: A structured illumination microscopy (SIM) system for generating single focal point patterns of a sample is disclosed. The SIM system performs a focusing, scaling and summing operation on each single focal point that completely scan the sample to produce a high resolution composite image.

Abstract: A resolution enhancement technique for a line scanning confocal microscopy system that generates vertical and horizontal line scanning patterns onto a sample is disclosed. The line scanning confocal microscopy system is capable of producing line scanning patterns through the use of two alternative pathways that generate either the vertical line scanning pattern or horizontal line scanning pattern.