Abstract: Disclosed herein are novel gene editing systems capable of being delivered to a subject intravenously through a lipid nano particle pharmaceutical formulation and producing durable in vivo editing of a target gene, such as ANGPTL3, with high on-target gene editing efficiency, reduced or low off-target effect, and no germline editing. The gene editing systems comprise a chemically modified guide nucleic acid sequence with a spacer having a specified arrangement of deoxyribonucleotides and ribonucleotides. The novel gene editing systems comprise mRNA that encodes the gene editor proteins, which may include a modified nickase component. Methods of disease treatments using the gene editing systems are also disclosed.

Abstract: The present invention is generally directed to systems and methods of providing automatic scaling pure-spot clusters. Such dusters may be dynamically rebalanced for further costs savings. In accordance with some methods of the present invention may include a method of utilizing a cluster in a big data cloud computing environment where instances may include reserved on-demand instances for a set price and on-demand spot instances that may be bid on by a user, the method including: creating one or more stable nodes, comprising spot instances with a bid price above a price for an equivalent on-demand instance; creating one or more volatile nodes, comprising spot instances with a bid price below a price for an equivalent on-demand instance; using one or more of the stable nodes as a master node; and using the volatile nodes as slave nodes.

Abstract: An electrical connector comprises at least one contact element, and a cover structure restricting access to the at least one contact element. The cover structure includes a wall element at least partially surrounding the at least one contact element and defines a reception zone between the wall element and the at least one contact element. The wall element includes at least one deflectable latch member. A cover element is movable within the reception zone along a retraction direction from a secured position to a retracted position. The at least one contact element is enclosed by the cover element in the secured position. The at least one deflectable latch member secures the cover element in the secured position.

Abstract: The present invention is generally directed to systems and methods of provisioning, and using heterogeneous clusters in a cloud-based big data system, the heterogeneous clusters made up of primary instance types and different types of instances, the method including: determining if there are composition requirements of any heterogeneous cluster, the composition requirements defining instance types permitted for use; determining if any of the permitted different types of instances are required or advantageous for use; determining an amount of different types of instances to utilize, this determination based at least in part on an instance weight; provisioning the heterogeneous cluster comprising both primary instances and permitted different types of instances.

Abstract: The present invention is generally directed to systems and methods of provisioning, and using heterogeneous clusters in a cloud-based big data system, the heterogeneous clusters made up of primary instance types and different types of instances, the method including: determining if there are composition requirements of any heterogeneous cluster, the composition requirements defining instance types permitted for use; determining if any of the permitted different types of instances are required or advantageous for use; determining an amount of different types of instances to utilize, this determination based at least in part on an instance weight; provisioning the heterogeneous cluster comprising both primary instances and permitted different types of instances.

Abstract: The present invention is generally directed to systems and methods of provisioning and using heterogeneous clusters in a cloud-based big data system, the heterogeneous clusters made up of primary instance types and different types of instances, the method including: determining if there are composition requirements of any heterogeneous cluster, the composition requirements defining instance types permitted for use; determining if any of the permitted different types of instances are required or advantageous for use; determining an amount of different types of instances to utilize, this determination based at least in part on an instance weight; provisioning the heterogeneous cluster comprising both primary instances and permitted different types of instances.

Abstract: The present invention is generally directed to systems and methods of provisioning and using heterogeneous clusters in a cloud-based big data system, the heterogeneous clusters made up of primary instance types and different types of instances, the method including: determining if there are composition requirements of any heterogeneous cluster, the composition requirements defining instance types permitted for use; determining if any of the permitted different types of instances are required or advantageous for use; determining an amount of different types of instances to utilize, this determination based at least in part on an instance weight; provisioning the heterogeneous cluster comprising both primary instances and permitted different types of instances.

Abstract: The present invention is generally directed to systems and methods of providing automatic scaling pure-spot clusters. Such dusters may be dynamically rebalanced for further costs savings. In accordance with some methods of the present invention may include a method of utilizing a cluster in a big data cloud computing environment where instances may include reserved on-demand instances for a set price and on-demand spot instances that may be bid on by a user, the method including: creating one or more stable nodes, comprising spot instances with a bid price above a price for an equivalent on-demand instance; creating one or more volatile nodes, comprising spot instances with a bid price below a price for an equivalent on-demand instance; using one or more of the stable nodes as a master node; and using the volatile nodes as slave nodes.