Abstract: A method and system for automated generation of test scenarios and automation scripts. The system is configured to record details of the application at micro level (page level), index based on the page navigation. The system is further configured to create a mind map or a tree using a traverse algorithm. This creates the necessary test scenarios based on flows or page navigation. At the same time, the system captures all the underlying screen properties and labels. The system connects the field type to the action library (built in) and also ensures a dataset is created while capturing the recording. Further utilizing the test scenarios, the dataset, and the screen properties, the system generates the automation script automatically.

Abstract: Automated software testing have become very crucial in this digital stage. It consumes a lot of manual effort and time of a tester. This disclosure relates generally to a method and system for automated generation of test scenarios and automation scripts. The system is configured to record details of the application at micro level (page level), index based on the page navigation. The system is further configured to create a mind map or a tree using a traverse algorithm. This creates the necessary test scenarios based on flows or page navigation. At the same time, the system captures all the underlying screen properties and labels. Connect the field type to the action library (built in). Also ensure dataset is created while capturing the recording. Further utilizing the test scenarios, data set and the screen properties to generate the automation script automatically.

Abstract: The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.

Abstract: The present disclosure relates to a method of reducing heterogeneity in antibodies during culturing, wherein the heterogeneity is due to proportion of charge variant of the antibody. The disclosure also comprises a process of growing cells in a cell culture system that results in antibodies with the reduced heterogeneity. In one embodiment antibody heterogeneity is reduced by the addition of divalent transitional metal ions such as zinc (Zn2+) to the cell culture medium. In another embodiment antibody heterogeneity is reduced by decreasing the osmolality of the cell culture medium.

Abstract: An imaging system for generating flexibly oriented electronic images includes an image sensor having a square pixel array, imaging optics for forming an optical image on at least a portion of the square pixel array, wherein the portion is within an image circle of the imaging optics and includes at least two rectangular sub-portions differing from each other in aspect ratio and/or orientation, and a processing module capable of generating an electronic image from each of the at least two rectangular sub-portions. An imaging method for generating electronic images of flexible orientation, using a square image sensor pixel array, includes forming an optical image on at least a portion of the square image sensor pixel array, selecting, according to a desired orientation, a rectangular sub-portion of the at least a portion of the square image sensor pixel array, and generating a final electronic image from the sub-portion.

Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogs, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.

Abstract: The invention relates to improvised pharmaceutical compositions permitting ingestion via oral delivery of proteins/peptides or their conjugates, and/or cation-insulin conjugate complexes demonstrating desirable pharmacokinetic profiles and potency in efficacy models of diabetes in dogs and humans. A preferred formulation comprises 0.01%-20% w/w of insulin, insulin compound conjugates and/or cation insulin conjugates, 10%-60% w/w of one or more fatty acid components selected from saturated or unsaturated C4-C12 fatty acids and/or salts of such fatty acids and additionally contains optimal amounts of other pharmaceutically suitable polymer excipients which permit improved solubility, dissolution rate and effective bioavailability of poorly water soluble compositions and consistent in-vivo release profiles upon scalability during manufacture. A further aspect of the invention features the process of preparing the aforesaid formulations.

Abstract: A computer implemented method for data archiving and dearchiving in a business environment is described herein. According to an implementation, the method includes obtaining metadata configuration associated with a source database storing transaction information, the transaction information being related to a business transaction. Based on the metadata configuration an archive specification is generated. Further, based on the archive specification and the metadata configuration, an archive mapping is configured. According to an aspect, the archive mapping is synchronized with an update in one of the metadata configuration. The archive mapping can be implemented for archiving the transaction information. Additionally, the archived transaction information can be de-archived, or can be purged onto a back-up repository.

Abstract: System and methods of data auditing of transaction data of an application software are described. In one implementation, the method includes data auditing of transaction data by an independent audit module where metadata of the application software is received, and an audit table based on at least one of the audit parameters, indicating the extent of audit data of one or more processes is created in an audit database of the audit module. Further, triggers for capturing data changes from the application software are generated based on at least one audit parameters. Captured changes data is optimized to reduce the storage requirements of audit data. In another implementation, the result of the analysis is displayed in form of a report in user desired format.

Abstract: The present invention relates to the preparation of insulin compounds including their analogs or derivatives thereof from their corresponding precursor forms by a one step enzymatic reaction involving the combinatorial and concurrent use of optimal quantities of trypsin and carboxypeptidase B that work synergistically directing the reaction in a controlled manner to avoid production of random undesired byproducts. Particularly, the enzymatic conversion reactions of the instant invention offer advantages of reduction in the number operational steps, higher yield and purity of the desired end products.

Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.

Abstract: An imaging system for generating flexibly oriented electronic images includes an image sensor having a square pixel array, imaging optics for forming an optical image on at least a portion of the square pixel array, wherein the portion is within an image circle of the imaging optics and includes at least two rectangular sub-portions differing from each other in aspect ratio and/or orientation, and a processing module capable of generating an electronic image from each of the at least two rectangular sub-portions. An imaging method for generating electronic images of flexible orientation, using a square image sensor pixel array, includes forming an optical image on at least a portion of the square image sensor pixel array, selecting, according to a desired orientation, a rectangular sub-portion of the at least a portion of the square image sensor pixel array, and generating a final electronic image from the sub-portion.

Abstract: The invention relates to methods of separation and/or purification of impurities yielding a purified heterologous protein product devoid of related impurities or with substantially minimal quantities of such glycosylated impurities. More specifically, the invention relates to the identification of glycosylated forms of insulin analogues such as glargine impurities characterized post expression in yeast based systems such as Pichia pastoris. The invention also relates to methods used to clone gene encoding the protein insulin glargine; inserting the related gene in a suitable yeast host; producing culture of the recombinant strain, stimulating expression of the heterologous polypeptide, its secretion and purification post fermentation and related enzymatic conversions.

Abstract: The present disclosure relates to a method of reducing heterogeneity in antibodies during culturing, wherein the heterogeneity is due to proportion of charge variant of the antibody. The disclosure also comprises a process of growing cells in a cell culture system that results in antibodies with the reduced heterogeneity. In one embodiment antibody heterogeneity is reduced by the addition of divalent transitional metal ions such as zinc (Zn2+) to the cell culture medium. In another embodiment antibody heterogeneity is reduced by decreasing the osmolality of the cell culture medium.

Abstract: The disclosure relates to method of reducing O-glycosylation levels of the insulin or insulin analog precursor molecule produced by Pichia sp. The present disclosure provides genetically engineered knock-out strains of methylotrophic yeast including Pichia and especially Pichia pastoris by disruption of Protein mannosyl transferase (PMT) genes and rendering them capable of producing heterologous proteins with reduced glycosylation. Vectors, which comprise coding sequences for PMT1, PMT2, PMT4, PMT5, and PMT6 genes, for transforming methylotrophic yeasts are contemplated by the present disclosure. PMT inactivated strains of this disclosure have been deposited at MTCC, Chandigarh. The strains are PMT1/GS115 (MTCC 5515), PMT4/GS115 (MTCC 5516), PMT5/GS115 (MTCC 5517) and PMT6/GS115 (MTCC 5518).

Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.

Abstract: The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.

Abstract: The invention provides a process for the production of lipase inhibitors via an improvised fermentation process characterized in that a combinatorial feeding of linoleic acid or its esters or salts thereof and an omega-9 fatty acid, preferably oleic acid and/or its derivatives is employed during said process resulting in an improved yield co-efficient, productivity further providing ease of operation.

Abstract: System and methods of data auditing of transaction data of an application software are described. In one implementation, the method includes data auditing of transaction data by an independent audit module where metadata of the application software is received, and an audit table based on at least one of the audit parameters, indicating the extent of audit data of one or more processes is created in an audit database of the audit module. Further, triggers for capturing data changes from the application software are generated based on at least one audit parameters. Captured changes data is optimized to reduce the storage requirements of audit data. In another implementation, the result of the analysis is displayed in form of a report in user desired format.