Patents by Inventor Harlan Saul Robins
Harlan Saul Robins has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10894977Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a template composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: GrantFiled: January 8, 2019Date of Patent: January 19, 2021Assignee: Adaptive Biotechnologies corporationInventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Publication number: 20190203281Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a template composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: ApplicationFiled: January 8, 2019Publication date: July 4, 2019Inventors: Harlan Saul ROBINS, Christopher Scott CARLSON, Robert J. LIVINGSTON, Ryan O. EMERSON, Anna M. SHERWOOD
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Patent number: 10214770Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: GrantFiled: May 10, 2016Date of Patent: February 26, 2019Assignee: ADAPTIVE BIOTECHNOLOGIES CORP.Inventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Patent number: 10150996Abstract: A relative representation of adaptive immune cells in a biological sample is quantified using multiplex PCR and sequencing of adaptive immune cells, control genes, and synthetic template molecules. Disclosed herein are methods for quantifying a number of adaptive immune cells in a biological sample, and methods for quantifying a relative representation of adaptive immune cells in a biological sample that comprises a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells. Methods are provided for amplifying by multiplex PCR and sequencing a first set of synthetic templates each comprising one TCR or IgV segment and one TCR of Ig J or C segment and a unique bar code.Type: GrantFiled: March 5, 2015Date of Patent: December 11, 2018Assignee: ADAPTIVE BIOTECHNOLOGIES CORP.Inventors: Harlan Saul Robins, Christopher Scott Carlson, Mark J. Rieder, Anna M. Sherwood, Ryan O. Emerson
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Publication number: 20170037469Abstract: A relative representation of adaptive immune cells in a biological sample is quantified using multiplex PCR and sequencing of adaptive immune cells, control genes, and synthetic template molecules. Disclosed herein are methods for quantifying a number of adaptive immune cells in a biological sample, and methods for quantifying a relative representation of adaptive immune cells in a biological sample that comprises a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells. Methods are provided for amplifying by multiplex PCR and sequencing a first set of synthetic templates each comprising one TCR or IgV segment and one TCR of Ig J or C segment and a unique bar code.Type: ApplicationFiled: March 5, 2015Publication date: February 9, 2017Inventors: Harlan Saul ROBINS, Christopher Scott CARLSON, Mark J. RIEDER, Anna M. SHERWOOD, Ryan O. EMERSON
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Publication number: 20160319340Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: ApplicationFiled: May 10, 2016Publication date: November 3, 2016Inventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Patent number: 9371558Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: GrantFiled: January 9, 2015Date of Patent: June 21, 2016Assignee: Adaptive Biotechnologies Corp.Inventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Patent number: 9150905Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: GrantFiled: May 8, 2013Date of Patent: October 6, 2015Assignee: Adaptive Biotechnologies CorporationInventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Publication number: 20150203897Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed N high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: ApplicationFiled: January 9, 2015Publication date: July 23, 2015Inventors: HARLAN SAUL ROBINS, CHRISTOPHER SCOTT CARLSON, ROBERT J. LIVINGSTON, RYAN O. EMERSON, ANNA M. SHERWOOD
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Publication number: 20150017652Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: ApplicationFiled: May 8, 2013Publication date: January 15, 2015Inventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston
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Publication number: 20140221220Abstract: A method of measuring immunocompetence is described. This method provides a means for assessing the effects of diseases or conditions that compromise the immune system and of therapies aimed to reconstitute it. This method is based on quantifying T-cell diversity by calculating the number of diverse T-cell receptor (TCR) beta chain variable regions from blood cells.Type: ApplicationFiled: April 14, 2014Publication date: August 7, 2014Applicant: Fred Hutchinson Cancer Research CenterInventors: Harlan Saul Robins, Edus H. Warren, III, Christopher Scott Carlson
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Publication number: 20140206549Abstract: A method of measuring immunocompetence is described. This method provides a means for assessing the effects of diseases or conditions that compromise the immune system and of therapies aimed to reconstitute it. This method is based on quantifying T-cell diversity by calculating the number of diverse T-cell receptor (TCR) beta chain variable regions from blood cells.Type: ApplicationFiled: February 18, 2014Publication date: July 24, 2014Applicant: Fred Hutchinson Cancer Research CenterInventors: Harlan Saul Robins, Edus H. Warren, III, Christopher Scott Carlson
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Publication number: 20140206548Abstract: A method of measuring immunocompetence is described. This method provides a means for assessing the effects of diseases or conditions that compromise the immune system and of therapies aimed to reconstitute it. This method is based on quantifying T-cell diversity by calculating the number of diverse T-cell receptor (TCR) beta chain variable regions from blood cells.Type: ApplicationFiled: February 18, 2014Publication date: July 24, 2014Applicant: Fred Hutchinson Cancer Research CenterInventors: Harlan Saul Robins, Edus H. Warren, III, Christopher Scott Carlson