Patents by Inventor Harold C. Riethman

Harold C. Riethman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods of specifically labeling nucleic acids using CRISPR/Cas
    Patent number: 10640810
    Abstract: Provided herein are methods of detecting a target nucleic acid sequence. In one embodiment, the method includes contacting genomic DNA with a guide RNA having a portion complementary to the target sequence in the genomic DNA and with Cas9 nickase to produce a single-strand break in the genomic DNA at a specific location adjacent to the target sequence. The method further includes contacting the nicked DNA with a polymerase and fluorescently labeled nucleotide. The fluorescently labeled nucleotide is incorporated into the nicked DNA at the specific location and the target nucleic acid sequence is detected via fluorescent label.
    Type: Grant
    Filed: October 18, 2017
    Date of Patent: May 5, 2020
    Assignees: Drexel University, The Wistar Institute of Anatomy and Biology, Temple University
    Inventors: Ming Xiao, Harold C. Riethman, Wenhui Hu, Jennifer McCaffrey
  • METHODS OF SPECIFICALLY LABELING NUCLEIC ACIDS USING CRISPR/CAS
    Publication number: 20180105867
    Abstract: Provided herein are methods of detecting a target nucleic acid sequence. In one embodiment, the method includes contacting genomic DNA with a guide RNA having a portion complementary to the target sequence in the genomic DNA and with Cas9 nickase to produce a single-strand break in the genomic DNA at a specific location adjacent to the target sequence. The method further includes contacting the nicked DNA with a polymerase and fluorescently labeled nucleotide. The fluorescently labeled nucleotide is incorporated into the nicked DNA at the specific location and the target nucleic acid sequence is detected via fluorescent label.
    Type: Application
    Filed: October 18, 2017
    Publication date: April 19, 2018
    Inventors: Ming Xiao, Harold C. Riethman, Wenhui Hu, Jennifer McCaffrey