Abstract: A mobile device captures an object image using the built in imaging capability of the device. A structural recognition module analyzes the captured image to identify the 3D shape of the captured object. A color recognition module analyzes the color of the captured image to differentiate between objects that may be similar in shape. The image analysis information is sent to a server and the server selects an appropriate video file for augmented reality (AR) display on the mobile device. User selection of an operational language for the mobile device may also provided to the server. The system includes audio data files in different languages corresponding to each video AR file. The server selects the audio file corresponding to the language selection data provided by the mobile device. The language appropriate audio file is linked to the video file and the video and audio AR data are transmitted to the mobile device.

Abstract: A mobile device captures an object image using the built in imaging capability of the device. A structural recognition module analyzes the captured image to identify the 3D shape of the captured object. A color recognition module analyzes the color of the captured image to differentiate between objects that may be similar in shape. The image analysis information is sent to a server and the server selects an appropriate video file for augmented reality (AR) display on the mobile device. User selection of an operational language for the mobile device may also provided to the server. The system includes audio data files in different languages corresponding to each video AR file. The server selects the audio file corresponding to the language selection data provided by the mobile device. The language appropriate audio file is linked to the video file and the video and audio AR data are transmitted to the mobile device.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: A method, a device, and a non-transitory storage medium that stores instructions to receive a user selection of a musical piece; provide performance cues to a user to perform musical events on a musical instrument, wherein the performance cues are synchronized to expert performance data of the musical piece; receive audio data corresponding to musical events performed by the user on the musical instrument; detect fundamental frequencies associated with the user-performed musical events; determine an extent to which the user-performed musical events have been correctly or incorrectly performed; and provide real-time or near real-time audio feedback and/or visual feedback indicating the extent to which the user-performed musical events have been correctly or incorrectly performed.

Abstract: Systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.

Abstract: A method includes receiving a user selection of a musical piece; providing performance cues to a user to perform musical events on a musical instrument, wherein the performance cues are synchronized to expert performance data of the musical piece; receiving audio data corresponding to musical events performed by the user on the musical instrument; detecting fundamental frequencies associated with the user-performed musical events; determining an extent to which the user-performed musical events have been correctly or incorrectly performed; providing real-time or near real-time audio feedback and/or visual feedback indicating the extent to which the user-performed musical events have been correctly or incorrectly performed; and using the expert performance data as real-time or near real-time audible or real-time or near real-time visual feedback; reporting user performance data of a session to a server; and storing the user performance data in a database.

Abstract: The Application relates to methods, and systems for nucleotide sequencing comprising producing polymerase reactions that comprise both catalytic and non-catalytic divalent metal ions. Polymerase/template/primer complexes are immobilized on a substrate. The complexes are exposed to a solution comprising a non-catalytic metal and nucleotides labeled with a detectable label on a portion of the nucleotide that is released upon incorporation. The cognate nucleotide is sequestered in the active site of the polymerase, unable to proceed to incorporation. After observing the sequestered cognate nucleotide, the complex is exposed to a catalytic metal, resulting in the incorporation of the bound cognate nucleotide and consequent release of the label resulting in a single-base extended primer.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: The subject mater herein relates to requesting data from a database and, more particularly, to database query table substitution. Various embodiments provide systems, methods, and software to evaluate requests for data from a database and make table substitutions in the request to reduce a number of tables queried. Some embodiments include receiving a data request including data from two or more database table columns and building a list of all database tables specified in the data request. Some such embodiments further include, for each column in the data request, identifying each table from the list of all tables within which the column is present and identifying a least number of one or more tables that together include all of the columns of the data request. The data request may then be modified by changing the table of one or more of the columns in the received data requests.

Abstract: The application relates to methods, and systems for nucleotide sequencing comprising producing polymerase reactions that comprise both catalytic and non-catalytic divalent metal ions. Polymerase/template/primer complexes are immobilized on a substrate. The complexes are exposed to a solution comprising a non-catalytic metal and nucleotides labeled with a detectable label on a portion of the nucleotide that is released upon incorporation. The cognate nucleotide is sequestered in the active site of the polymerase, unable to proceed to incorporation. After observing the sequestered cognate nucleotide, the complex is exposed to a catalytic metal, resulting in the incorporation of the bound cognate nucleotide and consequent release of the label resulting in a single-base extended primer.

Abstract: A method includes receiving a user selection of a musical piece; providing performance cues to a user to perform musical events on a musical instrument, wherein the performance cues are synchronized to expert performance data of the musical piece; receiving audio data corresponding to musical events performed by the user on the musical instrument; detecting fundamental frequencies associated with the user-performed musical events; determining an extent to which the user-performed musical events have been correctly or incorrectly performed; providing real-time or near real-time audio feedback and/or visual feedback indicating the extent to which the user-performed musical events have been correctly or incorrectly performed; and using the expert performance data as real-time or near real-time audible or real-time or near real-time visual feedback; reporting user performance data of a session to a server; and storing the user performance data in a database.

Abstract: A method includes receiving a user selection of a musical piece; providing performance cues to a user to perform musical events on a musical instrument, wherein the performance cues are synchronized to expert performance data of the musical piece; receiving audio data corresponding to musical events performed by the user on the musical instrument; detecting fundamental frequencies associated with the user-performed musical events; determining an extent to which the user-performed musical events have been correctly or incorrectly performed; providing real-time or near real-time audio feedback and/or visual feedback indicating the extent to which the user-performed musical events have been correctly or incorrectly performed; and using the expert performance data as real-time or near real-time audio and/or video feedback by controlling an output level of the expert performance data output to the user during a session.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog, are also provided. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: A graphical user interface (GUI) tool is presented to a user for interacting with an underlying database. The GUI tool includes a field selection and attribute selections for the field. The user selects a field and an attribute for that field and is presented with a first list of values retrieved from the database for the selected attribute. Next, the user selects a filter for the attribute within the GUI tool and a second reduced list of values is presented to the user within the GUI tool representing the filtered first list of values acquired by applying the filter.

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog, are also provided. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.