Patents by Inventor Harold R. Crews
Harold R. Crews has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7064823Abstract: The present invention is a flow cytometry-based hematology system useful in the analysis of biological samples, particularly whole blood or blood-derived samples. The system is capable of determining at least a complete blood count (CBC), a five-part white blood cell differential, and a reticulocyte count from a whole blood sample. The system preferably uses a laser diode that emits a thin beam to illuminate cells in a flow cell and a lensless optical detection system to measure one or more of axial light loss, low-angle forward scattered light, high-angle forward scattered light, right angle scattered light, and time-of-flight measurements produced by the cells. The lensless optical detection system contains no optical components, other than photoreactive elements, and does not include any moving parts. Finally, the system uses a unique system of consumable reagent tubes that act as reaction chambers, mixing chambers, and waste chambers for the blood sample analyses.Type: GrantFiled: May 31, 2002Date of Patent: June 20, 2006Assignee: IDEXX Laboratories, Inc.Inventors: John W. Roche, W. Peter Hansen, Michelle L. Coleman, Harold R. Crews
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Publication number: 20030030783Abstract: The present invention is a flow cytometry-based hematology system useful in the analysis of biological samples, particularly whole blood or blood-derived samples. The system is capable of determining at least a complete blood count (CBC), a five-part white blood cell differential, and a reticulocyte count from a whole blood sample. The system preferably uses a laser diode that emits a thin beam to illuminate cells in a flow cell and a lensless optical detection system to measure one or more of axial light loss, low-angle forward scattered light, high-angle forward scattered light, right angle scattered light, and time-of-flight measurements produced by the cells. The lensless optical detection system contains no optical components, other than photoreactive elements, and does not include any moving parts. Finally, the system uses a unique system of consumable reagent tubes that act as reaction chambers, mixing chambers, and waste chambers for the blood sample analyses.Type: ApplicationFiled: May 31, 2002Publication date: February 13, 2003Inventors: John W. Roche, W. Peter Hansen, Marcus F. Julian, Harold C. Flynn, James W. Russell, Michelle L. Coleman, Harold R. Crews
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Patent number: 5968831Abstract: The invention provides a cell control product for use with a cytoenzymology assay to confirm enzymatic activity in devices employing electronic and/or optical means. The cell control product comprises a lyophilized mammalian cell which is capable of being rehydrated in water to exhibit cellular structure and cellular enzymatic activity so that said lyophilized cells can function effectively as a cytoenzymology cell control in an enzymatic analysis. The cellular structure of the cell control is capable of being authenticated by light scatter or microscopy analysis and the cellular enzymatic activity is capable of being authenticated by fluorescence analysis. The cell control exhibits a real time stability when stored at between 2 and 8.degree. C. for at least a two month period of time. Preferably the mammalian cell is an abnormal cell selected from Molt 4, CCRF-CEM and HL-60 cell lines.Type: GrantFiled: October 29, 1996Date of Patent: October 19, 1999Assignee: Coulter International Corp.Inventors: Ravindra S. Shukla, Harold R. Crews, Adry Galiounghi, Eileen Landrum, Frank J. Lucas
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Patent number: 5905031Abstract: The necessity to detect blast cells in a patient sample to determine a treatment regime for leukemia has long been desired. The present invention relates to a method and assay reagents for identification of blast cells in a fluid sample. The method further enables the determination of the lineage of the blast cells. The new method provides a determination of blast cells in less time than prior art methods.Type: GrantFiled: November 1, 1996Date of Patent: May 18, 1999Assignee: Coulter International Corp.Inventors: Nazle Kuylen, Harold R. Crews, Gerald E. Jaffe, Maria Barcelon, Francis J. Lucas, Nancy M. Garcia
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Patent number: 5731206Abstract: A lytic reagent composition and a kit of a lytic reagent system are disclosed for the rapid isolation, identification and/or analysis of at least five subpopulations of leukocytes from a whole blood sample. The composition and system have application to any environment in which the study and/or analysis of the leukocyte fraction of whole blood requires their isolation in their native or near native state. The lytic reagent composition of this invention comprises saponin and a carboxylic acid of the formula RCOOH wherein R is H or a C.sub.1-3 aliphatic hydrocarbon radial optionally substituted by one or more carbonyl and/or hydroxy groups. The kit of a lytic reagent system comprises the lytic reagent composition and a quench reagent.Type: GrantFiled: March 29, 1996Date of Patent: March 24, 1998Assignee: Coulter Electronics, Inc.Inventors: Stephen L. Ledis, Harold R. Crews, Timothy J. Fischer, Ted Sena
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Patent number: 5560750Abstract: Disclosed are hair coloring compositions comprising coloring agent and disaccharide. The coloring agent preferably comprises a first oxidizing agent for bleaching the hair, a dyeing agent for adding color to the hair and a second oxidizing agent for developing the dyeing agent. Also disclosed are methods for altering the color of hair to a predetermined degree comprising (a) applying to the hair a hair coloring composition, said composition comprising a coloring agent and a disaccharide; (b) allowing said composition to remain in contact with the hair for a time sufficient to achieve the predetermined degree of color alteration; and (c) substantially removing said composition from the hair.Type: GrantFiled: June 7, 1995Date of Patent: October 1, 1996Assignee: Preemptive Advertising, Inc.Inventors: Harold R. Crews, Roy M. Evans, Jr., Joseph O. Rubert
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Patent number: 5415856Abstract: Disclosed are methods and compositions for the treatment of hair. Treating compositions comprising shaping agent and a non-reducing disaccharide are disclosed. The methods of the present invention comprise application of the composition to the hair for permanently altering the natural shape of the hair.Type: GrantFiled: August 14, 1991Date of Patent: May 16, 1995Assignee: Preemptive Advertising Inc.Inventors: Harold R. Crews, Roy M. Evans, Jr., Joseph O. Rubert
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Patent number: 5155044Abstract: A method and reagent system are disclosed for the rapid isolation, identification and/or analysis of leukocytes from a whole blood sample. The method and reagent system of this invention has application to any environment in which the study and/or analysis of the leukocyte fraction of whole blood requires their isolation in their native or near native state. One of the environments in which this invention can be used to advantage is in the performance of white cell differentiation on automated instrumentation designed for that purpose. In one of the preferred embodiments of this invention, the lytic reagent can contain a mixture of both formic and acetic acid, with the formic acid comprising the major component thereof and the acetic acid being present in only minor quantities, (if at all). This reagent system is used to selectively effect stromatolysis of red blood cells and create subtle modifications to the leukocyte population to enable their automated differentiation into five (5) sub-populations.Type: GrantFiled: December 12, 1991Date of Patent: October 13, 1992Assignee: Coulter Electronics, Inc.Inventors: Stephen L. Ledis, Harold R. Crews, Timothy J. Fischer, Ted Sena
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Patent number: 5101841Abstract: Disclosed are methods and compositions for the treatment of hair. Treating compositions comprising the amino acid cysteine and a non reducing disaccharide in solution are disclosed. The methods of the present invention comprise application of the aqueous solution to the hair for permanently altering the natural shape of the hair.Type: GrantFiled: May 8, 1990Date of Patent: April 7, 1992Inventors: Harold R. Crews, Roy M. Evans, Jr., Joseph O. Rubert
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Patent number: 4962038Abstract: An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.Type: GrantFiled: May 17, 1985Date of Patent: October 9, 1990Assignee: Coulter Electronics, Inc.Inventors: James H. Carter, Stephen L. Ledis, Harold R. Crews
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Patent number: 4947878Abstract: Disclosed are methods and compositions for the treatment of hair. Treating compositions comprising the amino acid cysteine and a non-reducing disaccharide in solution are disclosed. The methods of the present invention comprise application of the aqueous solution to the hair for permanently altering the natural shape of the hair.Type: GrantFiled: March 18, 1987Date of Patent: August 14, 1990Assignee: Preemptive Marketing, Inc.Inventors: Harold R. Crews, Roy M. Evans, Jr., Joseph O. Rubert
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Patent number: 4751179Abstract: This invention describes a reagent system, including saponin in a lysing reagent and a rapidly active cross-linking agent such as glutaraldehyde as a fixing reagent, which reproducably affects whole blood to cause the red blood cells to stromatolyze and modifies the leukocytes to generate data to define four distinct clusters for detection and classification by flow analysis instrumentation. The clusters represent the four major leukocyte types found in blood: lymphocytes, monocytes, neutrophils and eosinophils, thus providing a method of leukocyte differential analysis. The parameters used for the leukocyte classification include combinations of two or more of DC (Coulter) volume, high frequency (RF) size, opacity (RF size/DC volume), light scatter at various angular ranges, and fluorescence at various wavelengths of illumination.Type: GrantFiled: May 31, 1984Date of Patent: June 14, 1988Assignee: Coulter Electronics, Inc.Inventors: Stephen L. Ledis, Harold R. Crews, Ted Sena
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Patent number: 4528274Abstract: A method and reagent system is described for differential determination of more that one class of leukocyte populations, using an automatic blood cell analyzer. The reagent system includes a blood diluent and stromatolyzing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The stromatolyzing reagent is a mixture of an aqueous solution of quaternary ammonium salts, a chromagen forming agent and an additive which is a non-cationic surfactant. The quaternary ammonium salts, with the additive, serve the purpose of positioning, relative to one another and a volume reference point, the lymphoid and myeloid populations. The additive also acts to prevent or to reduce the amount of protein deposit in the sensing orifices of the analyzer, which deposit tends to accumulate from the cell debris resulting from blood cell stromatolyzation.Type: GrantFiled: March 25, 1983Date of Patent: July 9, 1985Assignee: Coulter Electronics, Inc.Inventors: James H. Carter, Stephen L. Ledis, Harold R. Crews, Ted Sena, Fred L. Larsen
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Patent number: 4521518Abstract: An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.Type: GrantFiled: July 6, 1982Date of Patent: June 4, 1985Assignee: Coulter Electronics, Inc.Inventors: James H. Carter, Stephen L. Ledis, Harold R. Crews
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Patent number: 4485175Abstract: A method and reagent system is described for differential determination of three-populations of leukocytes (lymphocyte, monocyte and granulocyte), using an automatic counting system. The reagent system includes a blood diluent and lysing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients at a preselected pH for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The lysing reagent is a mixture of an aqueous solution of quaternary ammonium salts, which lysing reagent is added to the diluted blood under more mild conditions of concentration and at a slower rate than is current practice in order to obtain an unexpected volumetric modification of at least one of the three-populations of leukocytes, whereby volumetric differential analysis can be accomplished.Type: GrantFiled: January 3, 1983Date of Patent: November 27, 1984Assignee: Coulter Electronics, Inc.Inventors: Stephen L. Ledis, Ted Sena, Harold R. Crews, James H. Carter, II
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Patent number: 4405719Abstract: This invention utilizes for stabilization of platelets a combination of iodoacetamide and an iminodiacetic acid or salt thereof, together with a compatible bacteriostatic agent, in an aqueous electrolytic solution which is maintained at a preselected range of pH and osmolality.In a preferred formulation, the stabilizing solution contains iodoacetamide, N-(2-acetamido)iminodiacetic acid and sodium penicillin. Ethylenediaminetetraacetic acid, or salts thereof, are optionally present as an additional ingredient.Type: GrantFiled: May 29, 1981Date of Patent: September 20, 1983Assignee: Coulter Electronics, Inc.Inventors: Harold R. Crews, James H. Carter, II, Ted Sena
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Patent number: 4389490Abstract: Stabilized reference control and calibrator compositions for determining multiple platelet parameters in stand alone platelet controls and whole blood reference controls are prepared from platelets stabilized with a fixative-stabilizing composition containing glutaraldehyde and a non-ionic surfactant which is a mixture of ethoxylates of certain isomeric linear alcohols.Type: GrantFiled: May 29, 1981Date of Patent: June 21, 1983Assignee: Coulter Electronics, Inc.Inventors: Harold R. Crews, James H. Carter, II, Ted Sena
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Patent number: 4384971Abstract: An isotonic cleansing agent and method for its use in electronic instrumentation systems for determining hematological components of blood samples are described. The cleansing agent comprises an aqueous solution of a non-hemolytic polyoxyethylated alkylphenol detergent, dimethylolurea, and an isotonic and isoconductive saline base which is free of phosphates. The dimethylolurea serves as a cell stabilizer, and also acts as a germicidal agent, together with other added germicidal agents, particularly sodium 1-hydroxypyridine-2-thione.Type: GrantFiled: August 24, 1981Date of Patent: May 24, 1983Assignee: Coulte Electronics, Inc.Inventors: James H. Carter, II, Harold R. Crews
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Patent number: 4358394Abstract: A process for preparing whole blood reference controls having long term stability up to six months for devices using electronic means for whole blood determinations including platelet count and mean cell volume and red blood distribution width. The preconditioning diluent for the red blood cells consists essentially of an aqueous solution of lactose, sodium azide, and a non-ionic surfactant and is pH buffered and osmolality adjusted. The media of the whole blood control includes lactose, fungicides and antibiotics, and supplementary agents including purine nucleosides. It also includes additional components which alter red blood cell membranes including bile salts and cholic acid derivatives, phenothiazine compounds and the salts thereof having antihistamine properties, and 4-aminobenzoic acid ester derivatives and their salts having local anesthetic properties.Type: GrantFiled: August 12, 1981Date of Patent: November 9, 1982Assignee: Coulter Electronics, Inc.Inventors: Harold R. Crews, David L. Chastain, Jr., Stephen L. Ledis
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Patent number: RE42143Abstract: The present invention is a flow cytometry-based hematology system useful in the analysis of biological samples, particularly whole blood or blood-derived samples. The system is capable of determining at least a complete blood count (CBC), a five-part white blood cell differential, and a reticulocyte count from a whole blood sample. The system preferably uses a laser diode that emits a thin beam to illuminate cells in a flow cell and a lensless optical detection system to measure one or more of axial light loss, low-angle forward scattered light, high-angle forward scattered light, right angle scattered light, and time-of-flight measurements produced by the cells. The lensless optical detection system contains no optical components, other than photoreactive elements, and does not include any moving parts. Finally, the system uses a unique system of consumable reagent tubes that act as reaction chambers, mixing chambers, and waste chambers for the blood sample analyses.Type: GrantFiled: March 6, 2007Date of Patent: February 15, 2011Assignee: IDEXX Laboratories, Inc.Inventors: John W. Roche, Peter W. Hansen, Michelle L. Coleman, Harold R. Crews