Abstract: Autologous fibrinogen and chitosan containing hemostatic adhesive agents having strong hemostatic properties when applied to a bleeding wound or vessel. Fibrinogen is isolated and purified using ammonium sulphate precipitation in slow incremental portions.

Abstract: An assembly especially suitable for performing biopsies on living organs or tissue is disclosed herein. The assembly includes a specifically designed open-ended hollow tubular member serving as a combination probe and guide, a specifically designed handle for holding the tubular member during performance of the biopsy and means including a needle positionable through the tubular member for collecting a specimen from the tissue. The assembly may also include means for controlling the depth of penetration of the needle into the tissue, means for preventing the tissue collected by the needle from passing entirely through the latter and a stylet serving both to protect the needle and as a means for applying local anesthetic to the organ or tissue to be biopsied.