Abstract: The present invention relates to the field of ribonucleic acid (RNA). More specifically, the present invention provides compositions and methods for highly multiplexed detection of pathogen-associated RNA. In a specific embodiment, a method for forming a target ribonucleic acid (RNA) proxy in a sample comprises the steps of (a) contacting a sample with one or more multi-partite probes that hybridize to a target RNA, wherein the one or more multi-partite probes comprise (i) a target capture probe, (ii) a 3?acceptor probe and (iii) a 5? phosphorylated donor probe; (b) incubating the sample of step (a) under conditions that allow hybridization of the one or more multi-partite probes to target RNA present in the sample; (c) immobilizing the target capture probes on a solid support; (d) washing away unbound multi-partite probes; and € ligating the acceptor probes and donor probes to form a target RNA proxy.

Abstract: The present invention relates to the field of proteases. More specifically, the present invention provides compositions and methods useful for profiling protease activity using phage display. In one embodiment, a display vector useful for profiling protease activity comprises a nucleic acid sequence encoding (a) a peptide to be displayed on the surface of the vector; (b) a first affinity tag C-terminal to the peptide; and (c) a second affinity tag N-terminal to the peptide. The display vector can comprise a virus, bacteriophage, yeast, bacteria, retrovirus, ribosome or mRNA. In particular embodiments, the peptide comprises a human peptidome library peptide.

Abstract: Provided herein are methods of detecting an antibody directed against a pathogen and uses thereof.

Abstract: The present invention relates to the field of allergies. More specifically, the present invention provides compositions and methods useful for identifying anti-allergen antibodies in a patient sample using phage display. In one embodiment, a method for detecting the presence of an antibody against an allergen in subject includes the steps of (a) contacting a reaction sample comprising a display library with a biological sample comprising antibodies, wherein the display library includes a plurality of peptides derived from a plurality of allergens; and (b) detecting at least one antibody bound to at least one peptide expressed by the display library, thereby detecting an antibody against the at least one peptide in the biological sample.

Abstract: Provided herein are methods of detecting an antibody directed against a pathogen and uses thereof.

Abstract: Described are methods for estimating the cross-sectional incidence or duration of infection of a virus. Method steps include obtaining a biological sample with antibodies from a subject having a viral infection. The biological sample is mixed with two or more epitopes or peptides from the proteins of a vims responsible for the viral infection. The amount of antibody binding to the epitopes or peptides is quantified and the cross-sectional incidence or duration of infection of a virus is estimated.

Abstract: The present invention relates to the field of proteases. More specifically, the present invention provides compositions and methods useful for profiling protease activity using phage display. In one embodiment, a display vector useful for profiling protease activity comprises a nucleic acid sequence encoding (a) a peptide to be displayed on the surface of the vector; (b) a first affinity tag C-terminal to the peptide; and (c) a second affinity tag N-terminal to the peptide. The display vector can comprise a virus, bacteriophage, yeast, bacteria, retrovirus, ribosome or mRNA. In particular embodiments, the peptide comprises a human peptidome library peptide.

Abstract: Provided herein are methods of detecting an antibody directed against a pathogen and uses thereof.

Abstract: Provided herein are methods of detecting an antibody directed against a pathogen and uses thereof. One aspect provided herein relates to a method for detecting an antibody against a pathogen in a subject, the method comprising: (a) contacting a reaction sample comprising a display library with a biological sample comprising antibodies, wherein the display library comprises a plurality of peptides derived from a plurality of pathogens, and (b) detecting a peptide bound to at least one antibody, thereby detecting an antibody capable of binding the peptide.

Abstract: Provided herein are methods of detecting an antibody directed against a pathogen and uses thereof.

Abstract: Provided herein are methods of detecting an antibody directed against a pathogen and uses thereof. One aspect provided herein relates to a method for detecting an antibody against a pathogen in a subject, the method comprising: (a) contacting a reaction sample comprising a display library with a biological sample comprising antibodies, wherein the display library comprises a plurality of peptides derived from a plurality of pathogens, and (b) detecting a peptide bound to at least one antibody, thereby detecting an antibody capable of binding the peptide.

Abstract: A method for fabricating hydrogel based microarrays by a nanostamping process is provided. A method of manufacturing a patterned hydrogel array is provided comprising the steps of contacting a patterned substrate with a hydrogel substrate to form a substrate complex, wherein the patterned substrate comprises a surface, a first polymer attached to the surface, and a second polymer reversibly attached to the first polymer, the hydrogel substrate comprises a polymer matrix and a plurality of attachment sites along the polymer matrix capable of attaching the second polymer, binding at least one attachment site to the second polymer, disassociating the dimer; and separating the substrate complex to obtain a patterned hydrogel array having a second polymer attached to an attachment site. Methods where the second polymer is synthesized using the first polymer as template are provided. Hydrogel arrays manufactured according to the methods of the invention are provided.