Patents by Inventor Harsha P. Gunawardena

Harsha P. Gunawardena has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cross-linking compositions and related methods of isotope tagging of interacting proteins and analysis of protein interactions
    Patent number: 9562010
    Abstract: An isotope labeled asymmetric cross-linker is provided for the detection of cross-linked peptides. A cross-linking and mass spectrometry strategy, referred to as isotope tagging of interacting proteins (iTIP), improves the specificity of detecting cross-linked peptides and accurate identification of the interacting peptide sequences via the incorporation of isotopic signatures that are readily observed in the MS/MS spectrum. Isotope tagged peptides can be identified using mass spectrometry based on doublet peaks in a spectrum. Spectra can be subjected to database search strategies available for the sequencing of linear or non-cross-linked peptides.
    Type: Grant
    Filed: January 22, 2014
    Date of Patent: February 7, 2017
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Harsha P. Gunawardena, Xian Chen
  • CROSS-LINKING COMPOSITIONS AND RELATED METHODS OF ISOTOPE TAGGING OF INTERACTING PROTEINS AND ANALYSIS OF PROTEIN INTERACTIONS
    Publication number: 20140206091
    Abstract: An isotope labeled asymmetric cross-linker is provided for the detection of cross-linked peptides. A cross-linking and mass spectrometry strategy, referred to as isotope tagging of interacting proteins (iTIP), improves the specificity of detecting cross-linked peptides and accurate identification of the interacting peptide sequences via the incorporation of isotopic signatures that are readily observed in the MS/MS spectrum. Isotope tagged peptides can be identified using mass spectrometry based on doublet peaks in a spectrum. Spectra can be subjected to database search strategies available for the sequencing of linear or non-cross-linked peptides.
    Type: Application
    Filed: January 22, 2014
    Publication date: July 24, 2014
    Applicant: The University of North Carolina at Chapel Hill
    Inventors: Harsha P. Gunawardena, Xian Chen