Patents by Inventor Haruhiko BITO

Haruhiko BITO has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Calcium reporter gene
    Patent number: 10836802
    Abstract: As a calcium indicator protein having an excellent fluorescent characteristic and calcium reactivity, there is provided DNA in which one of a nucleotide sequence derivative of a calmodulin-binding sequence (ckkap sequence) of calcium/calmodulin-dependent protein kinase kinase and a nucleotide sequence encoding a calcium-binding sequence (CaM sequence) of calmodulin is linked to a 5? end of a nucleotide sequence encoding a fluorescent protein, and the other nucleotide sequence is linked to a 3? end of the nucleotide sequence encoding the fluorescent protein. The calcium indicator protein encoded by this DNA, which based on the derivative of the ckkap sequence as a binding domain for the calcium-bound CaM sequence, exhibits a fluorescent characteristic and calcium reactivity superior to those of conventional calcium indicator proteins.
    Type: Grant
    Filed: June 8, 2015
    Date of Patent: November 17, 2020
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Haruhiko Bito, Masatoshi Inoue, Atsuya Takeuchi, Junichi Nakai, Masamichi Ohkura
  • CALCIUM REPORTER GENE
    Publication number: 20170152295
    Abstract: As a calcium indicator protein having an excellent fluorescent characteristic and calcium reactivity, there is provided DNA in which one of a nucleotide sequence derivative of a calmodulin-binding sequence (ckkap sequence) of calcium/calmodulin-dependent protein kinase kinase and a nucleotide sequence encoding a calcium-binding sequence (CaM sequence) of calmodulin is linked to a 5? end of a nucleotide sequence encoding a fluorescent protein, and the other nucleotide sequence is linked to a 3? end of the nucleotide sequence encoding the fluorescent protein. The calcium indicator protein encoded by this DNA, which based on the derivative of the ckkap sequence as a binding domain for the calcium-bound CaM sequence, exhibits a fluorescent characteristic and calcium reactivity superior to those of conventional calcium indicator proteins.
    Type: Application
    Filed: June 8, 2015
    Publication date: June 1, 2017
    Inventors: Haruhiko BITO, Masatoshi INOUE, Atsuya TAKEUCHI, Junichi NAKAI, Masamichi OHKURA