Abstract: There are provided Bacillus macerans decomposing turf pseudo thatch and thatch, a microbial mixture composition comprising 1 part by weight of the Bacillus microorganism and 5 to 30 parts by weight of clay minerals, and a microbial material comprising at least one compound selected from the group consisting of 50 to 100 parts by weight of an ionic surfactant, 50 to 100 parts by weight of casein and 50 to 100 parts by weight of a casein hydrolyzate, and 50 to 200 parts by weight of sugars, in 100 parts by weight of said microbial mixture composition. This microbial material decomposes thatch by transferring not only to turf pseudo thatch but also to the soil, also decomposes pseudo thatch layers without exerting any adverse effect on the soil and environments for living creatures, and in golf courses, can promote growth of turf.

Abstract: A method is disclosed for preparing an immobilized microorganism-complex having controlling activity against plant pathogenic microorganisms. The method comprises adding an inorganic salt of a polyvalent metal to a liquid containing microorganism cells antagonistic to plant pathogenic microorganisms, drying the resulting microorganism cells so as to obtain a powder, mixing the powdered microorganism cells with particles of humic acid obtained by extraction from compost to obtain aggregates of the microorganism cells, mixing the aggregates with an aqueous carrageenan solution, and then drying the resulting mixture.