Abstract: An olive component composition with anti-viral activity is disclosed. The olive component composition can be used to prevent, treat, or ameliorate symptoms of, viral infection.

Abstract: This invention teaches a novel treatment of patients infected with influenza virus in early stages of the disease, with liposomes called ?-gal/SA liposomes, in order to decrease the infection period and decrease further complications by this disease. The treatment is based on inhalation of biodegradable liposomes that present two types of carbohydrate epitopes: ?-Gal epitopes with the structure Gal?1-3Gal?1-4(3)GlcNAc-R) and sialic acid (SA) epitopes. The treatment is based on the ability of influenza virus to bind to SA epitopes and on the binding of the natural anti-Gal antibody (the most abundant natural antibody in humans) to ?-gal epitopes. Following inhalation of aerosolized ?-gal/SA liposomes they land in the mucus lining the respiratory tract. The ?-gal/SA liposomes bind influenza virus via SA epitopes interaction with hemagglutinin of the virus, thus they slow or prevent the progress of the influenza virus infection process.

Abstract: A method for producing an ?-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme; an influenza virus vaccine having a high effect (antigenicity), which is produced using an ?-Gal-expressing virus produced by the method; and others. Specifically disclosed are: a method for producing an ?-galactose epitope (Gal?1-3Gal?1-4GlcNAc-R: ?-Gal hereinafter)-expressing virus, which comprises the steps of: (1) introducing an ?1,3- galactosyltransferase gene in an expressible condition into a cell line that does not express ?-Gal to obtain a cell line capable of expressing ?-Gal; (2) inoculating a virus into the cell line capable of expressing ?-Gal to obtain a cell line infected with a virus; and (3) culturing the cell line infected with the virus to obtain a virus expressing ?-Gal from the culture medium..

Abstract: A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-?-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA of Clostridium perfringens as a template to thereby obtain a fragment of cDNA encoding endo-?-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5?-terminal and 3?-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.

Abstract: A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-?-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA of Clostridium perfringens as a template to thereby obtain a fragment of cDNA encoding endo-?-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5?-terminal and 3?-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.

Abstract: A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-?-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA of Clostridium perfringens as a template to thereby obtain a fragment of cDNA encoding endo-?-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5?-terminal and 3?-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.

Abstract: The present invention provides compositions and methods for inducing immune tolerance to one or more specific antigens in a host mammal. Generally, the methods involves engineering white blood cells, in vitro, to express an antigen which is not native to the host mammal. Cells engineered ex vivo are then introduced into the host mammal to induce immune tolerance to the expressed antigen.