Patents by Inventor Haruko Takeyama
Haruko Takeyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220270710Abstract: The present disclosure provides a system for automatically constructing and providing microbial genome data. This method for processing sequence information about a single biological unit includes: (A) a step for performing clustering, for each same lineage, on partial sequence information (in a slide, SAG) about a genome (or an equivalent gene set) of a plurality of single biological units (for example, cells), on the basis of a sequence (16S rRNA or a marker gene) for identifying biological lineage; and (B) a step for performing collation with information about a genome of the single biological units in a database, if necessary.Type: ApplicationFiled: April 24, 2020Publication date: August 25, 2022Inventors: Koji ARIKAWA, Masahito HOSOKAWA, Haruko TAKEYAMA, Masato KOGAWA, Keigo IDE
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Publication number: 20220195485Abstract: The present disclosure provides a technique enabling analysis of cell viability information and nucleic acid information together for each single cell in a cell population when analyzing the composition of the cell population. The present disclosure discloses a method that is for analyzing the composition of a cell population and that comprises: a step for providing gel capsules in each of which a single cell of the cell population is encapsulated in a state of being labeled with a labeling agent, where the labeling agent distinctively labels a living cell and a dead cell; a step for amplifying a nucleic acid derived from each single cell of the cell population; a step for obtaining cell viability information for each single cell of the cell population; and a step for analyzing the amplified nucleic acid derived from each single cell of the cell population.Type: ApplicationFiled: April 24, 2020Publication date: June 23, 2022Inventors: Masahito Hosokawa, Haruko Takeyama, Yohei Nishikawa, Masato Kogawa
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Patent number: 11142787Abstract: The present invention provides a genome library production method in which cell lysis and genome amplification are performed using a simple operation. More particularly, the present invention provides a method that is for amplifying polynucleotides in cells and that comprises: a step for using a sample containing two or more cells or cell-like structures, and encapsulating the cells or cell-like structures into droplets, one for each droplet; a step for gelling the droplets to generate gel capsules; a step for performing lysis of the cells or cell-like structures by immersing the gel capsules in one or more types of reagents for lysis so as to cause the polynucleotides in the cells to be eluted in the gel capsules and to be kept in the gel capsules in a state where substances binding to the polynucleotides are removed; and a step for bringing the polynucleotides into contact with a reagent for amplification to amplify the polynucleotides in the gel capsules.Type: GrantFiled: April 26, 2019Date of Patent: October 12, 2021Assignee: bitBiome, Inc.Inventors: Masahito Hosokawa, Haruko Takeyama, Yohei Nishikawa, Masato Kogawa
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Publication number: 20210246495Abstract: The present invention provides a genome library production method in which cell lysis and genome amplification are performed using a simple operation. More particularly, the present invention provides a method that is for amplifying polynucleotides in cells and that comprises: a step for using a sample containing two or more cells or cell-like structures, and encapsulating the cells or cell-like structures into droplets, one for each droplet; a step for gelling the droplets to generate gel capsules; a step for performing lysis of the cells or cell-like structures by immersing the gel capsules in one or more types of reagents for lysis so as to cause the polynucleotides in the cells to be eluted in the gel capsules and to be kept in the gel capsules in a state where substances binding to the polynucleotides are removed; and a step for bringing the polynucleotides into contact with a reagent for amplification to amplify the polynucleotides in the gel capsules.Type: ApplicationFiled: April 26, 2019Publication date: August 12, 2021Inventors: Masahito Hosokawa, Haruko Takeyama, Yohei Nishikawa, Masato Kogawa
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Patent number: 10927363Abstract: Alginate lyase activity is exhibited by the amino acid sequences (polypeptides) shown in SEQ ID No:1 and SEQ ID No:2. These polypeptides and their homology equivalents are used to produce 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) by contacting the polypeptide(s) with an alginate containing a uronic acid moiety and holding the mixture of the alginate and the polypeptide(s) at a temperature at which alginate lyase activity is exhibited.Type: GrantFiled: April 3, 2017Date of Patent: February 23, 2021Assignee: MIE UNIVERSITYInventors: Toshiyuki Shibata, Hideo Miyake, Yoshihiro Murase, Reiji Tanaka, Tetsushi Mori, Haruko Takeyama, Mami Takahashi
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Publication number: 20190127722Abstract: Alginate lyase activity is exhibited by the amino acid sequences (polypeptides) shown in SEQ ID No:1 and SEQ ID No:2. These polypeptides and their homology equivalents are used to produce 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) by contacting the polypeptide(s) with an alginate containing a uronic acid moiety and holding the mixture of the alginate and the polypeptide(s) at a temperature at which alginate lyase activity is exhibited.Type: ApplicationFiled: April 3, 2017Publication date: May 2, 2019Inventors: Toshiyuki SHIBATA, Hideo MIYAKE, Yoshihiro MURASE, Reiji TANAKA, Tetsushi MORI, Haruko TAKEYAMA, Mami TAKAHASHI
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Publication number: 20180238775Abstract: The purpose of the present invention is to provide a sample collection system capable of repeatedly collecting a sample. The present invention discloses a system including: at least one or multiple hollow collection needle(s) each having a knife edge for collecting the sample from a biological specimen; and a syringe, a solenoid and a reservoir that serve as a liquid-inflow mechanism allowing a solution to flow into the collection needle, wherein the sample is ejected from the collection needle together with the solution so as to recover the sample.Type: ApplicationFiled: March 30, 2018Publication date: August 23, 2018Applicant: FRONTIER BIO SYSTEMS, INC.Inventors: Hideki KAMBARA, Masahito HOSOKAWA, Haruko TAKEYAMA, Takuya YODA
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Publication number: 20090325258Abstract: Provided is a magnetic particle holding carrier enabling automatization of treatment of a biological substance such as a protein by improving dispersibility of nano-size magnetic particles and suppressing nonspecific adsorption onto the wall of a container such as a pipette tip without damaging the properties of the nano-size magnetic particles such as a large solid-phase area and ability to arbitrarily design a functional protein, and provide a method for preparing the same. The magnetic particle holding carrier is formed of a micro-size nonmagnetic carrier and a plurality of nano-size magnetic particles bound to the carrier.Type: ApplicationFiled: June 2, 2006Publication date: December 31, 2009Applicants: Tokyo University of Agriculture and Technology, Precision System Science Co,. LtdInventors: Tadashi Matsunaga, Haruko Takeyama, Tomoko Yoshino, Hideji Tajima
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Publication number: 20080287319Abstract: A separation method of single-stranded nucleic acid characterized in that nucleic acid amplification is performed using a first primer to which a second substance capable of binding specifically to a first substance is bound and a second primer to which the second substance is not bound, and double-stranded nucleic acid obtained by the nucleic acid amplification is bound to the first substance, and the double-stranded nucleic acid bound to the first substance is dissociated into a single strand, and a separation apparatus of single-stranded nucleic acid characterized by having a nucleic acid amplification part 1 for performing nucleic acid amplification using a first primer to which a second substance capable of binding specifically to a first substance is bound and a second primer to which the second substance is not bound, a binding part 2 for binding double-stranded nucleic acid obtained by the nucleic acid amplification to the first substance, and a dissociation part 3 for dissociating the double-strandedType: ApplicationFiled: February 27, 2006Publication date: November 20, 2008Applicant: YOKOGAWA ELECTRIC CORPORATIONInventors: Tadashi Matsunaga, Haruko Takeyama, Tsuyoshi Tanaka, Takeo Tanaami, Saya Sato, Hisao Katakura, Yuji Mitsumori, Takeyuki Mogi
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Method of extracting nucleic acid or protein using dendrimers and dendrimer-compositional substances
Patent number: 7405042Abstract: The present invention relates to a method of extracting nucleic acid or protein, in which multi-layer dendrimers are formed on the surface of fine particles, amino radicals for capturing nucleic acid or protein are formed on the surface of these dendrimers, and nucleic acid or protein is extracted using these amino radicals. The present invention can greatly and easily increase the recovery ratio of nucleic acid or protein.Type: GrantFiled: August 26, 2003Date of Patent: July 29, 2008Assignee: Yokogawa Electric CorporationInventors: Tadashi Matsunaga, Haruko Takeyama, Brandon Yoza, Kazuhisa Fukushima, Saya Satou -
Patent number: 7241624Abstract: The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.Type: GrantFiled: August 30, 2004Date of Patent: July 10, 2007Assignees: Yokogawa Electric CorporationInventors: Kazuhisa Fukushima, Saya Satou, Tadashi Matsunaga, Haruko Takeyama
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Publication number: 20060269961Abstract: The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.Type: ApplicationFiled: August 10, 2006Publication date: November 30, 2006Applicants: YOKOGAWA ELECTRIC CORPORATION, Tadashi MATSUNAGAInventors: Kazuhisa Fukushima, Saya Satou, Tadashi Matsunaga, Haruko Takeyama
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Publication number: 20050260600Abstract: The present invention relates to a method of extracting nucleic acid or protein, in which multi-layer dendorimers are formed on the surface of fine particles, amino radicals for capturing nucleic acid or protein are formed on the surface of these dendorimers, and nucleic acid or protein is extracted using these amino radicals. The present invention can greatly and easily increase the recovery ratio of nucleic acid or protein.Type: ApplicationFiled: August 26, 2003Publication date: November 24, 2005Applicants: Tadashi MATSUNAGA, YOKOGAWA ELECTRIC CORPORATIONInventors: Tadashi Matsunaga, Haruko Takeyama, Brandon Yoza, Kazuhisa Fukushima, Saya Satou
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Publication number: 20050130191Abstract: The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.Type: ApplicationFiled: August 30, 2004Publication date: June 16, 2005Applicants: YOKOGAWA ELECTRIC CORPORATION, TADASHI MATSUNAGAInventors: Kazuhisa Fukushima, Saya Satou, Tadashi Matsunaga, Haruko Takeyama
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Publication number: 20040048289Abstract: The object of the present invention is to provide Mms 16, a protein specific to a magnetic particle membrane derived from a magnetic bacterium (Magnetospirillum sp.) AMB-1, DNA which encodes the protein, and a sandwich immunoassay method and a pharmaceutical composition, etc., using the same. Mms 16, a novel protein specific to a bacterial magnetic particle membrane, was identified by: fractionating cell homogenate of magnetic bacteria into 3 fractions of cell membranes, magnetic particle membranes, and cytoplasms, and by comparing the SDS-PAGE profiles of each protein. Further, an amino acid sequence of the protein or a base sequence of a gene that encodes the protein was determined and it was confirmed that the protein exhibited GTPase activity.Type: ApplicationFiled: June 12, 2003Publication date: March 11, 2004Inventors: Takashi Matsunaga, Haruko Takeyama, Yoshiko Okamura