Patents by Inventor Harumi Masubuchi

Harumi Masubuchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleic acid detection by repetitive synthesis and degradation of a nucleotide on a nuclease resistant oligonucleotide
    Patent number: 5849487
    Abstract: The present invention relates to a method of detecting a polynucleotide, comprising hybridizing a polynucleotide of known nucleotide sequence with a nuclease-resistant oligonucleotide primer having a sequence complementary to a part of the polynucleotide, then adding at least one kind of deoxynucleoside triphosphate, DNA polymerase and nuclease thereto, synthesizing a complementary strand being a nucleotide species located adjacent to the 3'-terminal of the primer and complementary to the polynucleotide, followed by decomposition thereof, the synthesis and decomposition of the complementary strand being repeated one or more times, and detecting the resulting pyrophosphoric acid or deoxynucleoside monophosphate. The present invention also includes a detection kit used for this method of detecting a polynucleotide.
    Type: Grant
    Filed: January 29, 1996
    Date of Patent: December 15, 1998
    Assignees: Eiken Chemical Co., Ltd., Tanabe Seiyaku Co., Ltd.
    Inventors: Tetsu Hase, Harumi Masubuchi