Abstract: Provided is austenitic heat-resisting cast steel that is excellent in both of the heat resistance and the machinability. Austenitic heat-resisting cast steel, includes: C: 0.1 to 0.4 mass %; Si: 0.8 to 2.5 mass %; Mn: 0.8 to 2.0 mass %; S: 0.05 to 0.30 mass %; Ni: 5 to 20 mass %; N: 0.3 mass % or less; Zr: 0.01 to 0.20 mass %; Ce: 0.01 to 0.10 mass %; one type or more of the elements selected from the following groups of (i) to (iii), at least including (i), (i) Cr: 14 to 24 mass %, (ii) Nb: 1.5 mass % or less, and (iii) Mo: 3.0 mass % or less; and Fe and inevitable impurity as a remainder.

Abstract: Provided is austenitic: heat-resisting cast steel that is excellent in both of the heat resistance and the machinability. Austenitic heat-resisting cast steel, includes: C: 0.1 to 0.4 mass %; Si: 0.8 to 2.5 mass %, Mn: 0.8 to 2.0 mass %: S: 0.05 to 0.30 mass %, Ni: 5 to 20 mass %; N: 0.3 mass % or less; Zr: 0.01 to 0.20 mass %; Ce: 0.01 to 0.10 mass %; one type or more of the elements selected from the following groups of (i) to (iii), at least including (i), (i) Cr: 14 to 24 mass %, (ii) Nb: 1.5 mass % or less, and Mo: 3.0 mass % or less; and Fe and inevitable impurity as a remainder.

Abstract: Provided is austenite heat-resisting cast steel with reduced precipitation of ferrite phase during the application of thermal load to stabilize the austenite structure for improved heat resistance. The austenite heat-resisting cast steel contains elements of 0.1 to 0.6 mass % of C, 1.0 to 2.5 mass % of Si, 1.0 to 3.5 mass % of Mn, 0.05 to 0.2 mass % of S, 14 to 24 mass % of Cr, 5 to 20 mass % of Ni, 0.1 to 0.3 mass % of N, 0.01 to 1.2 mass % of Zr, 0.01 to 1.5 mass % of Cu, 0.01 to 1.5 mass % of Nb, Fe as a remainder and unavoidable impurity. The elements satisfy the following expressions: Mn—S?1.0; and C?(1/12Cr?32Zr)>0, where symbols for elements in these expressions represent values indicating content of the elements corresponding to the symbols in a unit of atomic %.

Abstract: Provided is austenite heat-resisting cast steel with reduced precipitation of ferrite phase during the application of thermal load to stabilize the austenite structure for improved heat resistance. The austenite heat-resisting cast steel contains elements of 0.1 to 0.6 mass % of C, 1.0 to 2.5 mass % of Si, 1.0 to 3.5 mass % of Mn, 0.05 to 0.2 mass % of S, 14 to 24 mass % of Cr, 5 to 20 mass % of Ni, 0.1 to 0.3 mass % of N, 0.01 to 1.2 mass % of Zr, 0.01 to 1.5 mass % of Cu, 0.01 to 1.5 mass % of Nb, Fe as a remainder and unavoidable impurity. The elements satisfy the following expressions: Mn—S?1.0; and C?(1/12Cr?32Zr)>0, where symbols for elements in these expressions represent values indicating content of the elements corresponding to the symbols in a unit of atomic %.

Abstract: A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

Abstract: A polypeptide having an RNase III activity with which the length of a dsRNA degradation product can be easily controlled depending on reaction conditions and, in preparing a dsRNA having a length allowing it to serve as an siRNA in RNA interference, a low-molecular weight product having little RNA interferring effect is scarcely formed; a method of degrading a dsRNA with the use of the above polypeptide; and a composition and a kit for the above method.

Abstract: A vector having a region encoding a cold shock protein gene mRNA-origin 5?-nontranslated region, characterized in that the 5?-nontranslated region has a mutation having been transferred therein so as to change the distance of the stem structure formed by the region.

Abstract: A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

Abstract: A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

Abstract: A protein having an activity of degrading a dsRNA, namely, being capable of acting on a long-chain dsRNA to form a dsRNA of a definite length; a method of efficiently preparing a dsRNA of a definite length which comprises treating a dsRNA with the protein having an activity of degrading a dsRNA in the coexistence of a protein having an activity of binding to a nucleic acid such as a protein having an RNA-binding activity; and a method of using the protein having an activity of binding to a nucleic acid to elevate the efficiency in an RNA synthesis reaction typified by dsRNA synthesis.

Abstract: A method of analyzing, in chromosomal DNA having a retroviral vector incorporated therein, DNA region derived from chromosome which is adjacent to the vector, characterized in that a primer extension reaction and a nucleic acid amplification reaction are carried out in the presence of a compound capable of lowering the Tm value of double stranded nucleic acid. There are further provided a kit and buffer for use in this method.

Abstract: A vector having a region encoding a cold shock protein gene mRNA-origin 5?-nontranslated region, characterized in that the 5?-nontranslated region has a mutation having been transferred therein so as to change the distance of the stem structure formed by the region.

Abstract: A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

Abstract: A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease-gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.

Abstract: Therapeutic or preventive agents for diseases due to the action of aldose reductase; and aldose reductase inhibitors, characterized by containing at least one compound having an aldose reductase inhibiting activity which is selected from among 2,5-dihydroxytetra-hydro-2-furancarboxylic acid, derivatives of the same, optical isomers of both and pharmacologically acceptable salts of them.

Abstract: A heat-resistant magnesium alloy exhibiting excellent heat resistance and castability, which comprises 1.0 to 6.0 % by weight of zinc, 0.4 to 1.0 % by weight of zirconium, 1.5 to 5.0 % by weight of rare earth element, up to 0.3 % by weight of calcium, magnesium being as the balance, and unavoidable impurities.

Abstract: A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.

Abstract: An isolated BalI restriction enzyme gene and an BalI isolated modification enzyme gene are disclosed. A process for preparing a large amount of BalI restriction enzyme which comprises culturing a transformant containing said genes and collecting BalI restriction enzyme from the culture broth is also disclosed.