Abstract: A method is provided for measuring glycated hemoglobin in a hemoglobin-containing sample which comprises reacting glycated hemoglobin in the hemoglobin-containing sample with an enzyme that catalyzes a reaction of oxidizing the glycated hemoglobin to generate hydrogen peroxide, in the presence of at least one anionic surfactant selected from the group consisting of N-acyl taurine in which a hydrogen atom of the amino group may be substituted with a substituent, alkyl sulfoacetic acid, polyoxyethylene alkyl ether acetic acid, N-acyl amino acid in which a hydrogen atom of the amino group may be substituted with a substituent, polyoxyethylene alkyl ether phosphoric acid, polyoxyethylene polycyclic phenyl ether phosphoric acid, alkyl phosphoric acid, and salts thereof, to generate hydrogen peroxide, and measuring the generated hydrogen peroxide.

Abstract: A method is provided for measuring glycated hemoglobin in a hemoglobin-containing sample which comprises: adding an enzyme that catalyzes a reaction of oxidizing glycated amino acid or glycated peptide to generate hydrogen peroxide without oxidizing the glycated hemoglobin, to the hemoglobin-containing sample to generate hydrogen peroxide; eliminating the generated hydrogen peroxide; adding an enzyme that catalyzes a reaction of oxidizing glycated hemoglobin to generate hydrogen peroxide thereto to generate hydrogen peroxide; and measuring the generated hydrogen peroxide.

Abstract: A method is provided for measuring glycated hemoglobin in a hemoglobin-containing sample which comprises reacting glycated hemoglobin in the hemoglobin-containing sample with an enzyme that catalyzes a reaction of oxidizing the glycated hemoglobin to generate hydrogen peroxide, in the presence of at least one anionic surfactant selected from the group consisting of N-acyl taurine in which a hydrogen atom of the amino group may be substituted with a substituent, alkyl sulfoacetic acid, polyoxyethylene alkyl ether acetic acid, N-acyl amino acid in which a hydrogen atom of the amino group may be substituted with a substituent, polyoxyethylene alkyl ether phosphoric acid, polyoxyethylene polycyclic phenyl ether phosphoric acid, alkyl phosphoric acid, and salts thereof, to generate hydrogen peroxide, and measuring the generated hydrogen peroxide.

Abstract: The present invention provides a method for preserving a leuco chromogen-containing aqueous solution comprising: adding at least one acid compound selected from the group consisting of a phosphoric acid compound, a carboxylic acid compound, a sulfonic acid compound, and a sulfuric acid compound to a leuco chromogen-containing aqueous solution; a method for stabilizing a leuco chromogen in an aqueous solution comprising: adding at least one acid compound selected from the group consisting of a phosphoric acid compound, a carboxylic acid compound, a sulfonic acid compound, and a sulfuric acid compound to a leuco chromogen-containing aqueous solution; and a liquid reagent comprising a leuco chromogen and at least one acid compound selected from the group consisting of a phosphoric acid compound, a carboxylic acid compound, a sulfonic acid compound, and a sulfuric acid compound.

Abstract: Described is a method for preserving an aqueous solution comprising a leuco chromogen, comprising adding at least one compound selected from the group consisting of polyoxyethylene alkylamine and polyoxyethylene alkenylamine to the aqueous solution containing a leuco chromogen, and a method for stabilizing a leuco chromogen, comprising allowing the leuco chromogen to coexist in an aqueous solution comprising at least one compound selected from the group consisting of polyoxyethylene alkylamine and polyoxyethylene alkenylamine.

Abstract: The present invention provides: a method for preserving an aqueous solution containing a leuco chromogen, comprising adding a compound having at least one substituent selected from the group consisting of a nitroso group and an azo group and having an ability to coordinate a metal ion or a salt thereof to the aqueous solution containing a leuco chromogen; a method for stabilizing a leuco chromogen, comprising allowing the leuco chromogen to coexist in an aqueous solution comprising a compound having at least one substituent selected from the group consisting of a nitroso group and an azo group and having an ability to coordinate a metal ion or a salt thereof; and a liquid reagent comprising a leuco chromogen and a compound having at least one substituent selected from the group consisting of a nitroso group and an azo group and having an ability to coordinate a metal ion or a salt thereof.

Abstract: It is to provide a method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising: reacting glycated hemoglobin in the hemoglobin-containing sample with a proteolytic enzyme in the presence of at least one salt selected from the group consisting of a pyridinium salt, a phosphonium salt, an imidazolium salt, and an isoquinolinium salt; reacting the obtained reaction product with fructosyl peptide oxidase; and measuring the generated hydrogen peroxide. The present invention provides a method for accurately measuring glycated hemoglobin in a hemoglobin-containing sample.

Abstract: Described is a method for accurately and highly sensitively measuring glycated hemoglobin in a hemoglobin-containing sample without being influenced by hemoglobin. It is related to a method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising: reacting hemoglobin-containing sample with a protease in the presence of a surfactant; and then reacting the obtained reaction product with fructosyl peptide oxidase, wherein the latter reaction or both of the former reaction and the latter reaction are performed in the presence of a halogen oxide, and measuring the generated hydrogen peroxide. The method for measuring glycated hemoglobin in a hemoglobin-containing sample provided by the present invention is useful in, for example, the measurement of glycated hemoglobin useful in the diagnosis of diabetes mellitus.

Abstract: Provided is a method for suppressing an effect of ascorbic acid on a measurement of glycated hemoglobin in a glycated hemoglobin-containing sample. The method of the present invention is a method for suppressing an effect of ascorbic acid on the measurement of glycated hemoglobin in a glycated hemoglobin-containing sample, comprising reacting the glycated hemoglobin-containing sample with a proteolytic enzyme, reacting a generated glycated peptide with a glycated peptide oxidase, and then measuring a generated hydrogen peroxide, wherein the reaction of the glycated peptide with the glycated peptide oxidase is performed in the presence of a halogen oxide and a substance selected from the group consisting of a substance represented by formula (I), wherein R1 represents substituted or unsubstituted alkyl and a substance represented by the general formula (II), wherein R2 represents substituted or unsubstituted alkyl; and represents a single bond or a double bond.

Abstract: The present invention provides: a method for preserving an aqueous solution containing a leuco chromogen, comprising adding a compound having at least one substituent selected from the group consisting of a nitroso group and an azo group and having an ability to coordinate a metal ion or a salt thereof to the aqueous solution containing a leuco chromogen; a method for stabilizing a leuco chromogen, comprising allowing the leuco chromogen to coexist in an aqueous solution comprising a compound having at least one substituent selected from the group consisting of a nitroso group and an azo group and having an ability to coordinate a metal ion or a salt thereof; and a liquid reagent comprising a leuco chromogen and a compound having at least one substituent selected from the group consisting of a nitroso group and an azo group and having an ability to coordinate a metal ion or a salt thereof.

Abstract: It is to provide a method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising reacting the hemoglobin-containing sample with a proteolytic enzyme in the presence of a surfactant, and then reacting the obtained reaction product with fructosyl peptide oxidase, wherein at least one of the former reaction and the latter reaction is performed in the presence of an isothiazolinone derivative; and measuring the generated hydrogen peroxide. The present invention provides a method for accurately and highly sensitively measuring glycated hemoglobin in a hemoglobin-containing sample without being influenced by hemoglobin.

Abstract: It is to provide a method for preserving an aqueous solution comprising a leuco chromogen, comprising adding at least one compound selected from the group consisting of polyoxyethylene alkylamine and polyoxyethylene alkenylamine to the aqueous solution containing a leuco chromogen, and a method for stabilizing a leuco chromogen, comprising allowing the leuco chromogen to coexist in an aqueous solution comprising at least one compound selected from the group consisting of polyoxyethylene alkylamine and polyoxyethylene alkenylamine. The present invention provides a method for preserving an aqueous solution comprising a leuco chromogen for stably preserving the leuco chromogen in an aqueous solution and a method for stabilizing a leuco chromogen.

Abstract: It is to provide a method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising: reacting glycated hemoglobin in the hemoglobin-containing sample with a proteolytic enzyme in the presence of at least one salt selected from the group consisting of a pyridinium salt, a phosphonium salt, an imidazolium salt, and an isoquinolinium salt; reacting the obtained reaction product with fructosyl peptide oxidase; and measuring the generated hydrogen peroxide. The present invention provides a method for accurately measuring glycated hemoglobin in a hemoglobin-containing sample.