Patents by Inventor Harvey C. Tian

Harvey C. Tian has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220016629
    Abstract: A microfluidic chip for on-chip detection of the presence or absence of a target nucleic acid region in an isolated nucleic acid sample is disclosed. The microfluidic chip includes a nucleic acid entanglement array, an isolated nucleic acid sample immobilized in the nucleic acid entanglement array, and at least one probe specific to a target nucleic acid region. Systems and methods of using the microfluidic chip are disclosed.
    Type: Application
    Filed: November 29, 2019
    Publication date: January 20, 2022
    Applicant: CORNELL UNIVERSITY
    Inventors: Harold G. CRAIGHEAD, Harvey C. TIAN, David M. LIN, Adam J. BISOGNI
  • Publication number: 20220010360
    Abstract: A method for isolating one or more distinct analyte component from a cell sample is disclosed, as well as processes for testing and analyzing the distinct analyte components. The distinct analyte components include: (i) a total protein fraction; (ii) a plasma membrane protein fraction; (iii) a total RNA fraction; (iv) a cytosolic RNA fraction; (v) a cytosolic protein fraction; (vi) a nuclear RNA fraction; (vii) a nuclear protein fraction; (viii) a chromatin fraction comprising genomic DNA (gDNA); (ix) a gDNA markers fraction. This method involves the use of microfluidic device having a cell capture component and a nucleic acid entanglement component, where the cell capture component includes a cell capture array having a plurality of cell capture micropillars, where the nucleic acid entanglement component includes a nucleic acid entanglement array having a plurality of nucleic acid entanglement micropillars, and where the microfluidic device operates under continuous flow conditions.
    Type: Application
    Filed: November 29, 2019
    Publication date: January 13, 2022
    Applicant: CORNELL UNIVERSITY
    Inventors: Harold G. CRAIGHEAD, Harvey C. TIAN, David M. LIN, Adam J. BISOGNI
  • Publication number: 20200316598
    Abstract: The present invention relates to, inter alia, a microfluidic device for performing single cell genomic DNA isolation and amplification under flow. The microfluidic device comprises a solid substrate having one or more microfluidic channel system formed therein. Each microfluidic channel system of the microfluidic device comprises: (a) an intake region comprising a single microchannel; (b) a plurality of cell segregation microchannels; (c) a cell capture site located downstream of each cell segregation microchannel; and (d) a DNA capture array positioned downstream of the cell capture site and comprising a plurality of micropillars. Also disclosed is a whole genome amplification system that includes the microfluidic device of the present disclosure, as well as a method for conducting single cell DNA analysis via on-chip whole genome amplification while under flow, and a method for multiple displacement amplification (MDA) reactions of one or more nucleic acid sequence isolated single cells.
    Type: Application
    Filed: May 22, 2017
    Publication date: October 8, 2020
    Applicant: CORNELL UNIVERSITY
    Inventors: Harold G. CRAIGHEAD, Harvey C. TIAN
  • Patent number: 9856513
    Abstract: The present invention relates to methods and arrays for use in high resolution imaging of individual nucleic acid molecules and chromatin fragments, including native chromatin fragments. In one aspect, the present invention relates to a chromatin array that includes a transfer platform having a support and a transfer surface layered on the support. The chromatin array also includes a plurality of elongated individual native chromatin fragments coupled to the transfer surface in an orderly pattern suitable for high resolution imaging of the plurality of native chromatin fragments. The native chromatin fragments of the chromatin array include both DNA and histones.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: January 2, 2018
    Assignee: CORNELL UNIVERSITY
    Inventors: Aline Cerf, Harold G. Craighead, Harvey C. Tian
  • Publication number: 20150011425
    Abstract: The present invention relates to methods and arrays for use in high resolution imaging of individual nucleic acid molecules and chromatin fragments, including native chromatin fragments. In one aspect, the present invention relates to a chromatin array that includes a transfer platform having a support and a transfer surface layered on the support. The chromatin array also includes a plurality of elongated individual native chromatin fragments coupled to the transfer surface in an orderly pattern suitable for high resolution imaging of the plurality of native chromatin fragments. The native chromatin fragments of the chromatin array include both DNA and histones.
    Type: Application
    Filed: January 28, 2013
    Publication date: January 8, 2015
    Applicant: CORNELL UNIVERSITY
    Inventors: Aline Cerf, Harold G. Craighead, Harvey C. Tian