Patents by Inventor Ha Ryoung Poo
Ha Ryoung Poo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8080402Abstract: The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability.Type: GrantFiled: January 11, 2007Date of Patent: December 20, 2011Assignees: Bioleaders Corporation, Korea Research Institute of Bioscience and Biotechnology, Chosun UniversityInventors: Moon-Hee Sung, Seung-Pyo Hong, Jong-Su Lee, Chang-Min Jung, Kyung-Soo Hahm, Dong-Gun Lee, Yoon Kyung Park, Chul-Joong Kim, Ha-Ryoung Poo
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Patent number: 7871816Abstract: Expression vectors that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-?-glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus. Methods for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.Type: GrantFiled: November 13, 2007Date of Patent: January 18, 2011Assignees: Bioleaders Corporation, Korea Research Institute of Bioscience and BiotechnologyInventors: Moon-Hee Sung, Ha Ryoung Poo, Jong Soo Lee, Chang Min Jung, Seung Pyo Hong, Chul-Joong Kim, Sue-nie Park, Hyun-mi Pyo
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Publication number: 20090117151Abstract: Expression vectors that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-?-glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus. Methods for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.Type: ApplicationFiled: November 13, 2007Publication date: May 7, 2009Applicants: BIOLEADERS CORP., Korea Research Institute of Bioscience and BiotechnologyInventors: Moon-Hee Sung, Ha Ryoung Poo, Jong-Soo Lee, Chang-Min Jung, Seong-Pyo Hong, Chul-Joong Kim, Sue-nie Park, Hyun-mi Pyo
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Patent number: 7425438Abstract: Expression vectors are described that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-?-glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus, Methodology for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.Type: GrantFiled: October 17, 2003Date of Patent: September 16, 2008Assignees: Bioleaders Corporation, Korea Research Institute of Bioscience and BiotechnologyInventors: Moon-Hee Sung, Ha Ryoung Poo, Jong-Soo Lee, Chang-Min Jung, Seong-Pyo Hong, Chul-Joong Kim, Sue-nie Park, Hyun-mi Pyo
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Publication number: 20080152615Abstract: The present invention relates to a composition for an immunopotentiator (adjuvant) containing poly-gamma-glutamic acid and a composition for a vaccine containing the immunopotentiator, and more particularly, to an immunopotentiator containing poly-gamma-glutamic acid capable of enhancing antibody production rate by administering it to an animal together with antigen having low immunogenicity, and a composition for a vaccine containing the immunopotentiator and antigen. The inventive adjuvant has almost no toxicity and side effects, and show high antibody titer even when it is used with antigen having poor immunogenicity, so it can be used by adding to medical composition including preventive or curative vaccine for non-contagious chronic diseases as well as cancer, especially prostatic carcinoma, colon carcinoma, lung cancer, breast cancer, ovarian cancer, head and neck cancer, pudendum cancer, bladder cancer, brain tumor and glioma.Type: ApplicationFiled: December 6, 2005Publication date: June 26, 2008Applicants: BIOLEADERS CORPORATION, M.D. LAB CO., LTD, Korea Research Institute Of Bioscience and BiotechnologyInventors: Moon-Hee Sung, Chul-Joong Kim, Ha Ryoung Poo, Seung Pyo Hong, Jong Soo Lee, Ji Youn Kim
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Publication number: 20080132440Abstract: The present invention relates to PGA-vitamin complex containing poly-?-glutamic acid(PGA), vitamin preparations containing said PGA-vitamin complex or cosmetic compositions. The inventive PGA-vitamin complex having excellent hygroscopicity, moisturizing property and skin compatibility, which is a complex of poly-?-glutamic acid and vitamin, has the effect of sustained-release as well as improves stability of vitamin having the functions, such as promotion of metabolism, anti-oxidation effect, protection of cell wall, increasing immunity, prevention of dry skin and keratinization, anti-wrinkles and moisturizing skin, thereby being useful as cosmetic compositions and sustained-release vitamin preparations for various applications.Type: ApplicationFiled: March 4, 2005Publication date: June 5, 2008Inventors: Moon-Hee Sung, Chung Park, Seok Chan Kim, Gyoo Soon Park, Hiroshi Uyama, Ha Ryoung Poo, Jae Jun Song
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Publication number: 20070253935Abstract: The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability.Type: ApplicationFiled: January 11, 2007Publication date: November 1, 2007Inventors: Moon-Hee Sung, Seung-Pyo Hong, Jong-Su Lee, Chang-Min Jung, Kyung-Soo Hahim, Dong-Gun Lee, Yoon Park, Chul-Joong Kim, Ha-Ryoung Poo
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Patent number: 7255855Abstract: The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Ana13 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability.Type: GrantFiled: February 27, 2004Date of Patent: August 14, 2007Assignee: Bioleaders CorporationInventors: Moon-Hee Sung, Seung-Pyo Hong, Jong-Su Lee, Chang-Min Jung, Kyung-Soo Hahm, Dong-Gun Lee, Yoon Kyung Park, Chul-Joong Kim, Ha-Ryoung Poo
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Publication number: 20060140971Abstract: The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.Type: ApplicationFiled: June 4, 2004Publication date: June 29, 2006Inventors: Moon-Hee Sung, Chul-Joong Kim, Chang-Min Jung, Seung-Pyo Hong, Jong Su Lee, Jae Choi, Kwang Kim, Kuroda Shunichi, Ha Ryoung Poo
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Publication number: 20060127447Abstract: The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95% of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang. The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.Type: ApplicationFiled: July 10, 2003Publication date: June 15, 2006Inventors: Moon-Hee Sung, Chung Park, Seung-Pyo Hong, Kwang Seok Kim, Jae Jun Song, Eu Gene Rha, Kwang Kim, Kenji Soda, Makoto Ashiuchi, Ha Ryoung Poo, Seung Lee
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Patent number: 7049097Abstract: An antibiotic-independent vector for stable vector maintenance and high protein expression and a method for gene expression using this vector are disclosed. The stable maintenance of the vector is due to expression of a glutamic acid racemase to complement a D-glutamic acid auxotroph. No antibiotics, such as ampicillin, are necessary for the stable maintenance of this vector.Type: GrantFiled: January 11, 2002Date of Patent: May 23, 2006Assignees: Bioleaders Corporation, Korea Research Institute of Bioscience and BiotechnologyInventors: Moon Hee Sung, Seung Goo Lee, Seung Pyo Hong, Eun Ja Yoon, Yoon Ho Choi, Ha Ryoung Poo
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Publication number: 20050249752Abstract: Expression vectors are described that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-?-glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus. Methodology for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.Type: ApplicationFiled: October 17, 2003Publication date: November 10, 2005Inventors: Moon-Hee Sung, Ha Ryoung Poo, Jong-Soo Lee, Chang-Min Jung, Seong-Pyo Hong, Chul-Joong Kim, Sue-nie Park, Hyun-mi Pyo
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Publication number: 20050191720Abstract: The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability.Type: ApplicationFiled: February 27, 2004Publication date: September 1, 2005Applicants: Bioleaders Corporation, Korea Research Institute of Bioscience and Biotechnology, Chosun UniversityInventors: Moon-Hee Sung, Seung-Pyo Hong, Jong-Su Lee, Chang-Min Jung, Kyung-Soo Hahm, Dong-Gun Lee, Yoon Park, Chul-Joong Kim, Ha-Ryoung Poo
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Publication number: 20030084474Abstract: The present invention relates to an antibiotic-independent vector for constant high-expression and a method for gene expression using this vector. The stable expression of a recombinant gene is maintained by a stable plasmid through the use of genes essential for the growth and proliferation of the microorganism as a marker, instead of antibiotics, such as ampicillin.Type: ApplicationFiled: September 11, 2002Publication date: May 1, 2003Inventors: Moon Hee Sung, Seung Goo Lee, Seung Pyo Hong, Eun Ja Yoon, Yoon Ho Choi, Ha Ryoung Poo