Abstract: Disclosed herein are methods of preparing composites from solid elastomer(s) and wet filler(s), as well as products, including composites, vulcanizates, and articles therefrom. The wet filler can have a liquid content of at least 15%. A resulting composite comprises the filler dispersed in the elastomer at a loading of at least 20 phr with a filler yield loss of no more than 10%, wherein the composite has a liquid content of no more than 10% by weight based on total weight of said composite.

Abstract: A method of preparing a composition for treating cancer can include collecting epidermal gel secretions of catfish, extracting the total lipid fraction from the freeze-dried epidermal gel secretions to provide a total lipid fraction including neutral lipids, glycolipids, and phospholipids. A neutral lipid fraction, a glycolipid fraction, and a phospholipid fraction can then be separated from the total lipid fraction. Each of the total lipid fraction, the neutral lipid fraction, the glycolipid fraction, and the phospholipid fraction can exhibit anti-inflammatory and/or anti-cancer activities.

Abstract: A method of treating cancer can include administering a therapeutically effective amount of a composition comprising a soluble protein fraction obtained from epidermal gel secretions of catfish to a patient in need thereof. The soluble protein fraction can include soluble proteins and lipids from the epidermal gel secretions of catfish. The soluble protein fraction can include about 87% of the soluble proteins and about 13% of the lipids. The cancer can include at least one of liver cancer and pancreatic cancer. The composition can be administered by intraperitoneal (IP) or sub-cutaneous (SC) injection.

Abstract: A method of treating cancer can include administering a therapeutically effective amount of a composition comprising a soluble protein fraction obtained from epidermal gel secretions of catfish to a patient in need thereof. The soluble protein fraction can include soluble proteins and lipids from the epidermal gel secretions of catfish. The soluble protein fraction can include about 87% of the soluble proteins and about 13% of the lipids. The cancer can include at least one of liver cancer and pancreatic cancer. The composition can be administered by intraperitoneal (IP) or sub-cutaneous (SC) injection.

Abstract: A method of preparing a therapeutic composition from the epidermal gelatinous secretion of catfish includes mixing catfish epidermal gel secretions (EGS) with phosphate buffered saline (pH 7.5) to provide an extract, homogenizing the extract to provide a homogenized extract, and centrifuging the homogenized extract to provide a soluble protein fraction and an insoluble protein fraction. The therapeutic composition can include about 87% soluble proteins and about 13% lipids.

Abstract: A method of treating damaged nerves can include administering a therapeutically effective amount of a composition comprising a soluble protein fraction obtained by fractionating epidermal gel secretions of catfish to a patient in need thereof. The soluble protein fraction can include about 87% soluble proteins and about 13% lipids. The composition can be administered by intraperitoneal (IP) or sub-cutaneous (SC) injection.

Abstract: A method of treating infertility in males can include administering a therapeutically effective amount of a composition comprising a soluble protein fraction obtained by fractionating epidermal gel secretions of catfish to a patient in need thereof. The soluble protein fraction can include soluble proteins and lipids obtained by fractionating the epidermal gel secretions of catfish. The soluble protein fraction can include 87% soluble proteins and 13% lipids. The composition can be administered by intraperitoneal (IP) or sub-cutaneous (SC) injection or sublingual (SL). A method of preparing the soluble protein fraction can include collecting the epidermal gel secretion of catfish and preparing a soluble protein fraction having 87% soluble proteins and 13% lipids by weight.

Abstract: A method of treating diabetes can include administering a therapeutically effective amount of a composition comprising a soluble protein fraction obtained from epidermal gel secretions of catfish to a patient in need thereof. The soluble protein fraction can include soluble proteins and lipids from the epidermal gel secretions of catfish. The soluble protein fraction can include about 87% of the soluble proteins and about 13% of the lipids. The composition can be administered by intraperitoneal (IP) or sub-cutaneous (SC) injection.