Abstract: Novel Mn2+-doped quantum dots are provided. These Mn2+-doped quantum dots exhibit excellent temperature sensitivity in both organic solvents and water-based solutions. Methods of preparing the Mn2+-doped quantum dots are provided. The Mn2+-doped quantum dots may be prepared via a stepwise procedure using air-stable and inexpensive chemicals. The use of air-stable chemicals can significantly reduce the cost of synthesis, chemical storage, and the risk associated with handling flammable chemicals. Methods of temperature sensing using Mn2+-doped quantum dots are provided. The stepwise procedure provides the ability to tune the temperature-sensing properties to satisfy specific needs for temperature sensing applications. Water solubility may be achieved by passivating the Mn2+-doped quantum dots, allowing the Mn2+-doped quantum dots to probe the fluctuations of local temperature in biological environments.

Abstract: A system and method for non-invasively tracking a particle in a sample is disclosed. The system includes a 2-photon or confocal laser scanning microscope (LSM) and a particle-holding device coupled to a stage with X-Y and Z position control. The system also includes a tracking module having a tracking excitation laser, X-Y and Z radiation-gathering components configured to detect deviations of the particle in an X-Y and Z directions. The system also includes a processor coupled to the X-Y and Z radiation gathering components, generate control signals configured to drive the stage X-Y and Z position controls to track the movement of the particle. The system may also include a synchronization module configured to generate LSM pixels stamped with stage position and a processing module configured to generate a 3D image showing the 3D trajectory of a particle using the LSM pixels stamped with stage position.

Abstract: A system and method for non-invasively tracking a particle in a sample is disclosed. The system includes a 2-photon or confocal laser scanning microscope (LSM) and a particle-holding device coupled to a stage with X-Y and Z position control. The system also includes a tracking module having a tracking excitation laser, X-Y and Z radiation-gathering components configured to detect deviations of the particle in an X-Y and Z directions. The system also includes a processor coupled to the X-Y and Z radiation gathering components, generate control signals configured to drive the stage X-Y and Z position controls to track the movement of the particle. The system may also include a synchronization module configured to generate LSM pixels stamped with stage position and a processing module configured to generate a 3D image showing the 3D trajectory of a particle using the LSM pixels stamped with stage position.

Abstract: Novel Mn2+-doped quantum dots are provided. These Mn2+-doped quantum dots exhibit excellent temperature sensitivity in both organic solvents and water-based solutions. Methods of preparing the Mn2+-doped quantum dots are provided. The Mn2+-doped quantum dots may be prepared via a stepwise procedure using air-stable and inexpensive chemicals. The use of air-stable chemicals can significantly reduce the cost of synthesis, chemical storage, and the risk associated with handling flammable chemicals. Methods of temperature sensing using Mn2+-doped quantum dots are provided. The stepwise procedure provides the ability to tune the temperature-sensing properties to satisfy specific needs for temperature sensing applications. Water solubility may be achieved by passivating the Mn2+-doped quantum dots, allowing the Mn2+-doped quantum dots to probe the fluctuations of local temperature in biological environments.

Abstract: A system that can maintain and track the position of a single nanoparticle in three dimensions for a prolonged period has been disclosed. The system allows for continuously imaging the particle to observe any interactions it may have. The system also enables the acquisition of real-time sequential spectroscopic information from the particle. The apparatus holds great promise in performing single molecule spectroscopy and imaging on a non-stationary target.

Abstract: A system that can maintain and track the position of a single nanoparticle in three dimensions for a prolonged period has been disclosed. The system allows for continuously imaging the particle to observe any interactions it may have. The system also enables the acquisition of real-time sequential spectroscopic information from the particle. The apparatus holds great promise in performing single molecule spectroscopy and imaging on a non-stationary target.

Abstract: The present invention relates to a strain of Escherichia coli (E. coli M2H) for biosynthesis of high yield carotenoids, the steps for obtaining E. coli M2H comprises: purifying the host strain JCL1613 for pCW9/P2IDI; create plasmids for producing carotenoid genes; transform the plasmids into JCL1613; utilizing physical method (UV) to induce and improve JCL1613; select the portions with 99% death rate; utilizing 50 mL shake flask incubation; and go through UV screening to obtain the mutated strain of E. coli M2H.

Abstract: A centrifugal blade unit of a cooling fan at least comprises a wheel hub and a plurality of fan blades connected around the circumference of the wheel hub, each having a first blade section and a second blade section that are either connected or bifurcated. Thereby, a reversed flow of a gaseous fluid through the fluid inlet of the cooling fan can be prevented.

Abstract: A memory cell manufacturing method includes the steps of: providing a silicon substrate with buried regions that are spaced by a channel; depositing a gate oxide layer over the substrate; removing a portion of the gate oxide layer which is over the channel; depositing a first polysilicon layer over remaining portions of the gate oxide layer and over exposed portion of the substrate; growing an insulating layer over the polysilicon layer; providing a first mask on the insulating layer, the mask having a length shorter than that of the insulating layer and two end portions which overlap respectively the buried regions; etching portions of the insulating layer and the polysilicon layer not covered by the mask; depositing a thin oxide layer over a remaining portion of the insulating layer; depositing a second polysilicon layer over the thin oxide layer and over the buried layers; providing a second mask, which has a width narrower than that of the second polysilicon layer, on the second polysilicon layer to defin

Abstract: A method and apparatus is described for providing extended subscriber features, e.g., equal access, to otherwise non-conforming central offices of a telephone switching system. According to the invention, a register module is interposed between the subscriber line and an input of the central office for intercepting digits from the subscriber line intended for the central office and for receiving subscriber information from the central office identifying the subscriber line. The register module includes a memory having a plurality of address locations and a control circuit for analyzing the intercepted digits to determine whether processing of the subscriber call requires an extended subscriber feature. If so, the intercepted digits are processed to generate a coded signal, the coded signal including the address location where the stored digits and the subscriber information resides in the memory.