Abstract: The present disclosure provides materials and methods related to image processing. In particular, the present disclosure provides methods for enhancing target signal detection using imaging processing analysis that identifies and removes non-specific background signals. The image processing methods of the present disclosure are useful for enhancing target signals in a variety of assays that involve fluorescent detection (e.g., fluorescent in situ hybridization, immunofluorescence).

Abstract: Disclosed herein are methods for detecting a target protein in a sample and methods for detecting a target protein and a target nucleic acid in a sample. Kits for carrying out the methods are also disclosed.