Abstract: A coating composition may include a polymeric binder and bacteria that exhibit biologically induced mineralization (BIM) or biologically controlled mineralization (BCM) of calcium carbonate (CaCO3) in the presence of environmental calcium. The bacteria may exhibit BIM or BCM of CaCO3 using the ChaA antiporter protein. The coating formulations may be used to form coatings that exhibit self-healing properties in response to damage, such as a cut, tear, puncture, abrasion, or the like. For example, in response to being exposed to the damage, the bacteria may utilize nutrients, a calcium source, and water to cause precipitation of CaCO3 at the site of the damage. The nutrients, the calcium source, and the water may be provided as part of the coating formulation, as part of another layer of a coating system, from an external source (e.g., an applied spray or wash), or combinations thereof.

Abstract: A method for sequestering CO2 and creating precipitated calcium carbonates includes: (a) providing a liquid calcification medium including: a nutrient broth including water and a yeast extract, a carbon source selected from calcium carboxylic acids and calcium dicarboxylic acids and mixtures thereof, and bacteria that naturally express the chaA gene; (b) introducing CO2 to the liquid calcification medium; and (c) allowing microbial induced carbonate precipitation of calcium carbonate, thereby sequestering at least some of the CO2 introduced in said step of introducing