Patents by Inventor He-Chul Ahn

He-Chul Ahn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • 3?-o-fluorescently modified nucleotides and uses thereof
    Patent number: 8030466
    Abstract: The present invention relates to a DNA sequencing method using a nucleoside triphosphate with a fluorescent blocking group on its 3?-OH end as a reversible terminator. Further, the present invention relates to sequencing-by-synthesis method using the mono-modified reversible terminator (MRT), the novel nucleotide monomer having a reversible fluorescent blocking group removable chemically or enzymatically at its 3?-OH end. The sequencing method of the present invention facilitates sequencing of bases inserted by terminating extension of a nucleotide chain by the nucleotide monomer and then detecting fluorescence signal from 3?-OH end. At this time, after analyzing the fluorescence signal, the blocking group conjugated to the 3?-OH end can be effectively removed, indicating that a free 3?-OH functional group can be successfully restored, so that the next monomer insertion is possible, making continuous sequencing possible.
    Type: Grant
    Filed: October 26, 2009
    Date of Patent: October 4, 2011
    Assignee: Korea Institute of Science and Technology
    Inventors: Dongyun Shin, Dae-ro Ahn, He-Chul Ahn
  • 3'-O-FLUORESCENTLY MODIFIED NUCLEOTIDES AND USES THEREOF
    Publication number: 20110076679
    Abstract: The present invention relates to a DNA sequencing method using a nucleoside triphosphate with a fluorescent blocking group on its 3?-OH end as a reversible terminator. Further, the present invention relates to sequencing-by-synthesis method using the mono-modified reversible terminator (MRT), the novel nucleotide monomer having a reversible fluorescent blocking group removable chemically or enzymatically at its 3?-OH end. The sequencing method of the present invention facilitates sequencing of bases inserted by terminating extension of a nucleotide chain by the nucleotide monomer and then detecting fluorescence signal from 3?-OH end. At this time, after analyzing the fluorescence signal, the blocking group conjugated to the 3?-OH end can be effectively removed, indicating that a free 3?-OH functional group can be successfully restored, so that the next monomer insertion is possible, making continuous sequencing possible.
    Type: Application
    Filed: October 26, 2009
    Publication date: March 31, 2011
    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Dongyun SHIN, Dae-Ro AHN, He-Chul AHN