Abstract: The present invention relates to a transdermal photocuring forming hydrogel with biological activity. The hydrogel can release recombinant collagen with biological activity, the recombinant collagen includes a sequence as shown in SEQ ID No. 1, an amino acid sequence of the recombinant collagen includes N basic repetitive units, and each basic repetitive unit includes n1 of the following characteristic amino acid sequences: “G-Xaa1-Xaa2-G-E-Xaa3”; the 3? end and the 5? end of the basic repetitive unit are connected to form the above characteristic amino acid sequence. The recombinant collagen provided by the present invention has relatively obvious integrin binding activity, and has the effects of promoting cell adhesion, proliferation and differentiation. The hydrogel of the present invention has good biocompatibility and stable quality, which can be cured and formed in situ in a transdermal photo-crosslinking manner after injection, with simple, convenient and controllable operation.

Abstract: A method for determining tobacco-specific nitrosamines (TSNAs) in cigarette smoke using one-step clean-up coupled with LC-MS/MS is provided, including the following steps: collecting a particulate matter of mainstream cigarette smoke with a Cambridge filter pad, mixing the particulate matter of mainstream cigarette smoke, an internal standards solution and water in a 50 mL plastic centrifuge tube, and vortexing the resulting mixture at room temperature to allow extraction; transferring an extraction solution after filtration to a new centrifuge tube, adding dichloromethane, and vortexing the resulting mixture; centrifuging to collect a dichloromethane extraction solution in a lower layer to another centrifuge tube, and placing the centrifuge tube in a water bath to remove dichloromethane; dissolving the resulting extraction solution in water, and transferring the resulting solution to an autosampler vial for LC-MS/MS analysis.

Abstract: A method for determining tobacco-specific nitrosamines (TSNAs) in tobacco using one-step clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) is provided, including the following steps: destemming flue-cured tobacco or sun-cured tobacco, drying, milling, and passing through a sieve; mixing the resulting tobacco, an internal standards solution and water in a plastic centrifuge tube, and vortexing the resulting mixture at room temperature to allow extraction; transferring an extraction solution after filtration to a new centrifuge tube, adding dichloromethane, and vortexing the resulting mixture; centrifuging to collect a dichloromethane extraction solution in a lower layer to another centrifuge tube, and placing the centrifuge tube in a water bath to remove dichloromethane; dissolving the resulting extraction solution in water, and transferring the resulting solution to an autosampler vial for LC-MS/MS analysis.

Abstract: The application provides a mounting mechanism for a hidden vehicle door handle and a vehicle, and belongs to the technical field of vehicles. The mounting mechanism comprises an outer plate mounting assembly, a base assembly and tolerance absorption assemblies; the outer plate mounting assembly is configured to be disposed on the inner side of a vehicle door outer plate and integrally disposed on the vehicle door outer plate; the base assembly is configured to be connected with a handle and clamped and fixed to a designated mounting position by the outer plate mounting assembly; and the tolerance absorption assemblies are fixedly connected with the base assembly, disposed between mounting holes and bolts on a vehicle door outer plate reinforcing plate, and configured to absorb tolerances between the bolts and the mounting holes.

Abstract: An inductor having a coaxial structure is described. In one example, the structure of the single-turn inductor can include a conductor, an insulation layer, a shielding layer, and a magnetic core. An air duct can be located between the shielding layer and the magnetic core. The shielding layer and the magnetic core can both be connected to a ground. In one example, the single-turn inductor can include a single-layer termination structure formed on terminations of the shielding layer. In another example, the single-turn inductor can include a double-layer termination structure formed on terminations of the shielding layer. Displacement current in the single-turn inductor can be reduced using, for example, lumped equivalent circuit models, a semi-conductive shielding layer model, or a resistive layer and conductive shielding layer model.

Abstract: The application discloses five polymorph forms B, P, F, J, O of (4-((R)-((2S,5R)-4-(3-fluorobenzyl)-(2,5-dimethylpiperazine-1-yl)(3-hydroxyphenyl)methyl)phenyl)(4-methylpiperidine-1-yl)methanone dihydrochloride, preparation methods thereof and application thereof in the manufacture of a medicament for preventing or treating a mood disorder or a disease related to a ? opioid receptor.

Abstract: Provided is a card-making substrate, a preparation method thereof and an IC card or electronic tag containing the same. The card-making substrate comprises an aluminum laminated film layer, a bonding adhesive layer and a base material layer that are stacked in sequence. At least one continuous scratch is formed on a surface of the aluminum laminated film layer. The scratch has a depth greater than the thickness of the aluminum film in the aluminum laminated film layer. The aluminum film in the aluminum laminated film layer is divided into at least two regions that are not connected with each other by the scratch and the edge of the aluminum laminated film layer.

Abstract: The application discloses five polymorph forms B, P, F, J, O of (4-((R)-((2S,5R)-4-(3-fluorobenzyl)-(2,5-dimethylpiperazine-1-yl)(3-hydroxyphenyl)methyl)phenyl)(4-methylpiperidine-1-yl)methanone dihydrochloride, preparation methods thereof and application thereof in the manufacture of a medicament for preventing or treating a mood disorder or a disease related to a ? opioid receptor.

Abstract: The invention relates to a method for the production of adenosine 3?,5?-monophosphate (cAMP) by a permeable microbial strain which uses polyols for protecting activities of enzymes and a cAMP precursor and phosphate as substrates. Glucose is used as an energy provider and metal ions and an organic solvent such as acetone are used in the medium.

Abstract: Disclosed is a method for the production of cyclic adenosine 3?,5?-monophosphate (cAMP) by permeable microbial strain, which uses polyols for protecting activities of enzymes, uses cAMP precursor and phosphate radical ions as substrates, uses glucose as energy provider, and uses metal ions in the mendium. The method is simple, economical and has high yield.