Abstract: A baculovirus vector and a use thereof in the preparation of a recombinant adeno-associated virus (rAAV) in an insect cell are provided. The baculovirus vector includes an exogenous gene expression cassette and a stable sequence. The stable sequence is located at a site 5 kb or less from the exogenous gene expression cassette, and the stable sequence is a conserved noncoding element (CNE) sequence or a nucleocapsid assembly-essential element (NAE) sequence. When an insect cell is infected with a recombinant baculovirus (rBV) constructed in this way, after multiple continuous passages, production levels of the rBV and the rAAV still remain relatively stable.

Abstract: An expression cassette containing overlapping open reading frames and an application thereof are provided. The overlapping open reading frames are overlapping open reading frames of a first ORF and a second ORF and include in sequence from a 5? end to a 3? end: a first promoter at least used to drive gene transcription of the first ORF; a 5? part of a gene of the first ORF; an intron; and a 3? part of a gene of the second ORF, the intron including a second promoter used only to drive gene transcription of the second ORF. By arranging two promoters in a single expression cassette in the disclosure, the two promoters are used to drive the expression of proteins of the overlapping reading frames and regulate the relative expression time and expression intensity of different proteins.

Abstract: Embodiments of the present disclosure provide a method, device, and medium for placing information. The method comprises obtaining a plurality of pairs of consumption limits and actual consumptions within a period of time, where the consumption limits in the plurality of pairs are not monotonic in a case that the plurality of pairs are sorted by actual consumptions. The method further comprises adjusting the consumption limits in the plurality of pairs such that the adjusted consumption limits are monotonic in the case that the plurality of pairs are sorted by the actual consumptions. In addition, the method further comprises allocating a target consumption limit for placement of target information based on the adjusted plurality of pairs of consumption limits and actual consumptions.

Abstract: The disclosure discloses an expression cassette for expressing a gene including overlapping open reading frames in an insect cell and an application thereof. The expression cassette includes from 5? to 3? and operably linked: a promoter capable of driving transcription in the insect cell; an artificially constructed sequence; the overlapping open reading frames missing only a first translation start codon; wherein the artificially constructed sequence includes a native or engineered intron with splicing activity in the insect cell, the intron includes ATG or the intron is located between any two adjacent nucleotides in ATG. A recombinant adeno-associated virus vector including the expression cassette of the disclosure regulates relative expressions of VP1, VP2, and VP3 proteins, and relative expressions of Rep78 and Rep52 proteins by using a designed intron sequence and through an intron splicing function for large-scale production of rAAV.

Abstract: A detection method of key road-sections based on Ricci flow is provided and includes: building a weighted road network according to static road network data and actual traffic flow data; calculating initial values of Olivier Ricci curvature at different times; obtaining a weight system of making edges of the weighted road network be with a same value of Olivier Ricci curvature by a Ricci flow iterative process; and calculating direction and degree of weight change of each of the edges corresponding to road-sections, and setting a threshold to extract key road-sections. The method solves problems that the existing methods analyze the key road-sections from the topological structure of the road network without fully considering the actual distribution and transmission characteristics of the traffic flow in the network, is simple and easy; and the detection result more meet the traffic distribution and the flow of the actual road-sections.

Abstract: The disclosure belongs to the field of genetic engineering and discloses an expression cassette of a gene including overlapping open reading frames and an application thereof in an insect cell. The expression cassette includes from 5? to 3? and operably linked: a promoter capable of driving transcription in the insect cell; an artificially constructed sequence; overlapping open reading frames missing only a first translation start codon; wherein the artificially constructed sequence includes a first intron and a second intron with splicing activity in the insect cell, the first intron and the second intron share a 5? part or a 3? part, and the artificially constructed sequence does not include a translation start codon; during a post-transcriptional processing process, through an alternative splicing function of the first intron and the second intron, a translation start codon AUG can be formed.

Abstract: An electronic apparatus for establishing a Dual-Stack Lite (DS-lite) tunnel is provided. The apparatus sends a request for an Internet Protocol (IP) address of a Domain Name System (DNS) server and a domain name of an Address Family Transition Router (AFTR) server to a Dynamic Host Configuration Protocol (DHCP) server using an IP address of the DHCP server, receives the IP address of the DNS server and the domain name of the AFTR server from the DHCP server in response to the request, sends a DNS query including the domain name of the AFTR server to the DNS server using the IP address of the DNS server. In response to the DNS query being successful, the apparatus receives an IP address of the AFTR server from the DNS server, and establishes the DS-lite tunnel between the apparatus and the AFTR server using the IP address of the AFTR server.

Abstract: An electronic apparatus for establishing a Dual-Stack Lite (DS-lite) tunnel is provided. The apparatus sends a request for an Internet Protocol (IP) address of a Domain Name System (DNS) server and a domain name of an Address Family Transition Router (AFTR) server to a Dynamic Host Configuration Protocol (DHCP) server using an IP address of the DHCP server, receives the IP address of the DNS server and the domain name of the AFTR server from the DHCP server in response to the request, sends a DNS query including the domain name of the AFTR server to the DNS server using the IP address of the DNS server. In response to the DNS query being successful, the apparatus receives an IP address of the AFTR server from the DNS server, and establishes the DS-lite tunnel between the apparatus and the AFTR server using the IP address of the AFTR server.

Abstract: An approach includes the use of a description of instructions for invoking hardware accelerator and for a hardware accelerator to execute those instructions. In some embodiments, the instructions for invoking hardware accelerator and for a hardware accelerator to execute those instructions are described using a single language. These descriptions are then compiled into other languages for use in tool chains for generating simulators (a hardware and instruction set simulator and a hardware accelerator simulator). In some embodiments, the approach illustrated herein can be combined with state machine functionality to manage the execution of instructions that require multiple states. In some embodiments, the approach illustrated herein can be combined with an external register file for transferring information between a processor and a hardware accelerator.

Abstract: An electronic apparatus for establishing a Dual-Stack Lite (DS-lite) tunnel is provided. The apparatus sends a request for an Internet Protocol (IP) address of a Domain Name System (DNS) server and a domain name of an Address Family Transition Router (AFTR) server to a Dynamic Host Configuration Protocol (DHCP) server using an IP address of the DHCP server, receives the IP address of the DNS server and the domain name of the AFTR server from the DHCP server in response to the request, sends a DNS query including the domain name of the AFTR server to the DNS server using the IP address of the DNS server. In response to the DNS query being successful, the apparatus receives an IP address of the AFTR server from the DNS server, and establishes the DS-lite tunnel between the apparatus and the AFTR server using the IP address of the AFTR server.

Abstract: An electronic apparatus for establishing a Dual-Stack Lite (DS-lite) tunnel is provided. The apparatus sends a request for an Internet Protocol (IP) address of a Domain Name System (DNS) server and a domain name of an Address Family Transition Router (AFTR) server to a Dynamic Host Configuration Protocol (DHCP) server using an IP address of the DHCP server, receives the IP address of the DNS server and the domain name of the AFTR server from the DHCP server in response to the request, sends a DNS query including the domain name of the AFTR server to the DNS server using the IP address of the DNS server. In response to the DNS query being successful, the apparatus receives an IP address of the AFTR server from the DNS server, and establishes the DS-lite tunnel between the apparatus and the AFTR server using the IP address of the AFTR server.

Abstract: A detection method of key road-sections based on Ricci flow is provided and includes: building a weighted road network according to static road network data and actual traffic flow data; calculating initial values of Olivier Ricci curvature at different times; obtaining a weight system of making edges of the weighted road network be with a same value of Olivier Ricci curvature by a Ricci flow iterative process; and calculating direction and degree of weight change of each of the edges corresponding to road-sections, and setting a threshold to extract key road-sections. The method solves problems that the existing methods analyze the key road-sections from the topological structure of the road network without fully considering the actual distribution and transmission characteristics of the traffic flow in the network, is simple and easy; and the detection result more meet the traffic distribution and the flow of the actual road-sections.

Abstract: An endoscopic cannula, including: a handle; a sheath; a core rod disposed in the sheath and capable of sliding along an axial direction of the sheath. The core rod includes a first channel, a second channel, and a third channel. The handle includes a first joint configured to guide a lead wire into the first channel, a second joint configured to guide water into the second channel, and a third joint configured to guide an endoscope into the third channel. The sheath includes a first end and a second end, and the second end is disposed in the handle.

Abstract: The present invention provides a human Tim-3-Ig fusion protein which can block Tim-3 signal pathway, and said Ig fusion protein comprises Tim-3 protein, human Ig fragment, and the linking sequence therebetween. In the present invention, a human Tim-3-Ig gene is obtained by an artificial synthesis process; an expression vector containing the Tim-3-Ig gene is constructed; and the prepared Ig fusion protein is tested in an expression verification experiment, a binding activity experiment, a blocking activity experiment in different cell lines, and in vivo experiments in mice. The human Tim-3-Ig fusion protein prepared in the present invention can be used to treat immunological diseases caused by high expression of Tim-3.

Abstract: The present invention provides a human Tim-3-Ig fusion protein which can block Tim-3 signal pathway, and said Ig fusion protein comprises Tim-3 protein, human Ig fragment, and the linking sequence therebetween. In the present invention, a human Tim-3-Ig gene is obtained by an artificial synthesis process; an expression vector containing the Tim-3-Ig gene is constructed; and the prepared Ig fusion protein is tested in an expression verification experiment, a binding activity experiment, a blocking activity experiment in different cell lines, and in vivo experiments in mice. The human Tim-3-Ig fusion protein prepared in the present invention can be used to treat immunological diseases caused by high expression of Tim-3.

Abstract: Providing search results is disclosed. A search query is received and a plurality of preprocessing functions to be performed on the search query is determined based at least in part on a search label corresponding to the search query. The plurality of preprocessing functions is performed on the search query to obtain a plurality of preprocessed search queries. Based at least in part on precision levels associated with the plurality of preprocessed search queries, a search plan comprising a selected preprocessed search query is generated. The search plan is input to the search engine to obtain the search results.

Abstract: Various embodiments of a method, system, and apparatus related to query based on vertical search are disclosed. In one aspect, a method of query based on vertical search receives a user query. The method obtains a first category model from a category model warehouse based on the user query to generate a first query result. The first category model includes one or more commodity categories that correspond to one or more keywords in the user query. The method also obtains one or more commodity categories corresponding to the user query from a commodity warehouse to generate a second query result. The method further generates a final query result by combining the first query result and the second query result.

Abstract: Various embodiments of a method, system, and apparatus related to query based on vertical search are disclosed. In one aspect, a method of query based on vertical search receives a user query. The method obtains a first category model from a category model warehouse based on the user query to generate a first query result. The first category model includes one or more commodity categories that correspond to one or more keywords in the user query. The method also obtains one or more commodity categories corresponding to the user query from a commodity warehouse to generate a second query result. The method further generates a final query result by combining the first query result and the second query result.