Abstract: Provided are methods for isolating biomolecules, such as nucleic acids and proteins, from a sample using a silica-containing surface and/or a high salt, low pH buffer.

Abstract: The present disclosure provides methods for isolating nucleic acids from a sample, comprising: (a) contacting a sample, a lysate of the sample, a supernatant of the lysate, or a portion of the sample, the lysate or the supernatant with one or more first agents (e.g., protein precipitating agents) and one or more second agents (e.g., inhibitor removing agents) to generate a mixture, (b) separating the mixture of step (a) into a solid phase and a liquid phase, wherein the one or more second agents are primarily in the solid phase, and (c) isolating nucleic acids from the liquid phase of step (b). Compositions and kits useful in such methods are also disclosed. Further disclosed are methods, compositions and kits for preparing a lysate using a lytic reagent comprising one or more relatively mild chaotropic agents and one or more phosphates from a sample, especially a complex sample, such as a soil or stool sample.

Abstract: A synthetic molecule can be added to a sample at a specified concentration to accurately and/or precisely quantify target molecules included in the sample. The synthetic molecule can include a number of nucleotides. Some of the regions of the synthetic molecule can include sequences that correspond to primers used in an amplification process and other regions of the synthetic molecule can include sequences that are machine-generated. In implementations, an initial number of target molecules included in the sample can be determined based on a number of the target molecules included in an amplification product in relation to the number of synthetic molecules added to the sample.

Abstract: Provided are methods for isolating biomolecules, such as nucleic acids and proteins, from a sample using a silica-containing surface and/or a high salt, low pH buffer.

Abstract: Provided are methods for isolating biomolecules, such as nucleic acids and proteins, from a sample using a silica-containing surface and/or a high salt, low pH buffer.

Abstract: Provided are methods and compositions for isolating protein or other biomolecules from biological or environmental samples. The isolated biomolecules are substantially free of contaminants.

Abstract: Provided are methods and compositions for isolating protein or other biomolecules from biological or environmental samples. The isolated biomolecules are substantially free of contaminants.

Abstract: The present disclosure provides methods for isolating proteins and optionally nucleic acids from a sample, comprising: (a) contacting a sample, a lysate of the sample, a supernatant of the lysate, or a portion of the sample, the lysate or the supernatant with one or more first agents selected from low molecular weight carboxylates and sulfate and one or more second agents that are multivalent (e.g., trivalent) salt(s) to generate a mixture, (b) separating the mixture of step (a) into a solid phase and a liquid phase, wherein the one or more second agents are primarily in the solid phase, and (c) isolating proteins and optionally nucleic acids from the liquid phase of step (b). Compositions and kits useful in such methods are also disclosed.

Abstract: Provided are methods for isolating biomolecules, such as nucleic acids and proteins, from a sample using a silica-containing surface and/or a high salt, low pH buffer.

Abstract: Provided are methods and compositions for isolating protein or other biomolecules from biological or environmental samples. The isolated biomolecules are substantially free of contaminants.

Abstract: This invention is directed to a composition comprising 4-chloro-3,6-dinitrobenzotrifluoride and related compounds and method for using it to treat infections caused by organisms that have microtubules.