Abstract: The invention is directed an array comprising at least one depurination probe including a depurination sensitive first cleavage site adjacent a first tag region; and a base-cleavable second cleavage site adjacent a second tag region wherein the first tag region and the second tag region are independently quantifiable upon release from the depurination probe; and a method for use thereof to quantify depurination side reactions and overall yield during in situ array synthesis.

Abstract: Methods for fabricating chemical arrays are provided. Aspects of the invention include methods for providing solid activator in feature locations of a substrate surface. Aspects of the invention also include substrates having solid activator present in feature locations thereof, arrays produced from the substrates, methods for use of the arrays and kits that include the arrays.