Abstract: The present invention discloses a PIS genotype detection method for goats and belongs to field of life science. The method includes 4 pairs of specific primers: SRY F and SRY R; PIS wt F and PIS wt R; PIS var-1 F and PIS var-1 R; and PIS var-2 F and PIS var-2 R, and a probe. The present invention further provides a specific detection method and a detection standard. A simple and efficient detection method for diagnosing a PIS genotype of the goats is developed in combination with a high-sensitivity PCR method and a high-stability Southern Blot method, has extremely high specificity, is convenient and rapid, and may be used for accurately identifying genotypes of PIS genes, guiding breeding and mating of the goats and controlling or adjusting genotype frequencies of the PIS genes in goat populations according to requirements of breeders, to increase economic benefits of goat farming.

Abstract: The present invention provides a method of site-directed insertion to H11 locus in pigs by using site-directed cutting system, includes the following steps: 1) identify the targeted sequence targeted by the targeted cutting system in the targeted genome sequence of pigs; 2) design and construct the targeting sequence of the corresponding cutting system according to the targeted site; 3) construction of targeting vector; 4) transfect cells, identify the efficiency of fixed-point insertion by PCR amplification. The invention is dependent on the site-directed cutting system of H11 locus in pigs, to insert the target gene into the target site, in order to solve the problems such as low efficiency of traditional shooting technique, inconvenience design of PCR detection primer, harder to detect. The invention provides a method of site-directed insertion which can stably express the foreign gene at the H11 locus, to build an efficient platform for the production of transgenic pigs.