Patent number: 11993792
Abstract: The present disclosure relates, according to some embodiments, to systems, apparatus, compositions, methods, and workflows that include DNase I variants with desirable properties including, for example, salt tolerance. A DNase I variant, in some embodiments, may have an amino acid sequence that is at least 85% identical, at least 90% identical, at least 95% identical, and/or at least 98% identical to SEQ ID NO:1 and may be identical to SEQ ID NO:1 at one or more positions selected from the group of positions corresponding to L29, A35, D87, Q88, S94, P103, T108, P121, P132, A135, D145, E161, G172, P190, H208, and A224 of SEQ ID NO:1.
Type:
Grant
Filed:
May 27, 2021
Date of Patent:
May 28, 2024
Assignee:
New England Biolabs, Inc.
Inventors:
Heidi Crosby, Jennifer Ong, Ashley Luck, Eric J. Cantor, Vladimir Potapov
Publication number: 20220380741
Abstract: The present disclosure relates, according to some embodiments, to systems, apparatus, compositions, methods, and workflows that include DNase I variants with desirable properties including, for example, salt tolerance. A DNase I variant, in some embodiments, may have an amino acid sequence that is at least 85% identical, at least 90% identical, at least 95% identical, and/or at least 98% identical to SEQ ID NO:1 and may be identical to SEQ ID NO:1 at one or more positions selected from the group of positions corresponding to L29, A35, D87, Q88, S94, P103, T108, P121, P132, A135, D145, E161, G172, P190, H208, and A224 of SEQ ID NO:1.
Type:
Application
Filed:
May 27, 2021
Publication date:
December 1, 2022
Applicant:
New England Biolabs, Inc.
Inventors:
Heidi Crosby, Jennifer Ong, Ashley Luck, Eric J. Cantor, Vladimir Potapov
