Abstract: Submersible technology capable of sampling and evaluating suspended particulate materials, including living and dead organisms, is disclosed herein. In addition to fluorescent imaging and analysis, the invention provides automated cell staining, cell sorting, particulate concentration, and organism recovery and preservation. These new technologies may be incorporated into a traditional “fully vertical flow path” cytometer configuration most suitable for anchored or suspended stationary embodiments, or in a novel modularized “low relief” configuration, suitable for integration into or on a submersible aquatic vehicle or in other low clearance installations, where it is important to limit the vertical height of the device.

Abstract: Submersible technology capable of sampling and evaluating suspended particulate materials, including living and dead organisms, is disclosed herein. In addition to fluorescent imaging and analysis, the invention provides automated cell staining, cell sorting, particulate concentration, and organism recovery and preservation. These new technologies may be incorporated into a traditional “fully vertical flow path” cytometer configuration most suitable for anchored or suspended stationary embodiments, or in a novel modularized “low relief” configuration, suitable for integration into or on a submersible aquatic vehicle or in other low clearance installations, where it is important to limit the vertical height of the device.

Abstract: Submersible technology capable of sampling and evaluating suspended particulate materials, including living and dead organisms, is disclosed herein. In addition to fluorescent imaging and analysis, the invention provides automated cell staining, cell sorting, particulate concentration, and organism recovery and preservation. These new technologies may be incorporated into a traditional “fully vertical flow path” cytometer configuration most suitable for anchored or suspended stationary embodiments, or in a novel modularized “low relief” configuration, suitable for integration into or on a submersible aquatic vehicle or in other low clearance installations, where it is important to limit the vertical height of the device.

Abstract: The systems, methods, and apparatus described herein use a combination of video and flow cytometric technology to both capture images of organisms for identification and measure chlorophyll fluorescence associated with each image. Images can be automatically classified with software based on a support vector machine, while the measurements of chlorophyll fluorescence allow us to more efficiently analyze phytoplankton cells by triggering on chlorophyll-containing particles. Quantitation of chlorophyll fluorescence in large phytoplankton cells enables the interpretation of patterns in bulk chlorophyll data, and the discrimination of heterotrophic and phototrophic cells.