Abstract: The invention relates to a method for producing three-dimensional, preferably porous, hydrogel structures by a layer build-up technique, wherein the method includes the following steps. Providing (S1) of a liquid hydrogel solution, preferably a liquid alginate solution, and a, preferably transportable, sample carrier. Layerwise applying (S2) the liquid hydrogel solution onto the sample carrier in a temperature environment, the temperature of which is below the freezing point of the hydrogel solution, to produce a frozen 3D layered hydrogel structure. In order to increase advantageously the porosity of the 3D layered hydrogel structure, i.e. the proportion of small voids, cavities and/or depressions in the 3D layered hydrogel structure, the method further includes the step of drying (S3) of the frozen 3D layered hydrogel structure, e.g. by freeze-drying, to produce a porous 3D hydrogel structure. The invention further relates to a device for the layerwise building-up of three-dimensional hydrogel structures.

Abstract: The invention relates to a method for producing three-dimensional, preferably porous, hydrogel structures by means of layer build-up technique, wherein the method comprises the following steps. Providing (S1) of a liquid hydrogel solution, preferably a liquid alginate solution, and a, preferably transportable, sample carrier. Layerwise applying (S2) the liquid hydrogel solution onto the sample carrier in a temperature environment, the temperature of which is below the freezing point of the hydrogel solution, to produce a frozen 3D layered hydrogel structure. In order to increase advantageously the porosity of the 3D layered hydrogel structure, i.e. the proportion of small voids, cavities and/or depressions in the 3D layered hydrogel structure, the method according to the invention further comprises the step of drying (S3) of the frozen 3D layered hydrogel structure, e.g. by means of freeze-drying, to produce a porous 3D hydrogel structure.

Abstract: The invention relates to a method for producing vascularized biological tissue, having the steps of producing a network structure made of a plurality of interconnected filaments (11) of a support polymer, coating the network structure with a protein material, populating the coated network structure with endothelial cells (2, 2A) and tissue-forming biological cells (3), and dissolving the filaments (11) such that the vascularized tissue (1) is formed. The vascularized tissue (1) comprises cardiomyocytes, liver cells, renal cells, nerve cells, and/or pancreatic cells, for example.

Abstract: The invention relates to a method implemented in a control system of a machine for processing input values in an overall system with a working level and an evaluation level comprising inputting first input values to the working level and determining first output values; forming first situation data; inputting the first situation data to the evaluation level and determining first evaluations indicating whether the first situation data satisfy predetermined first conditions; influencing the determination of the first output values based on the first evaluations; inputting second input values to the working level and determining second output values, wherein the determination of the second output values is influenced by the first output values; forming second situation data; inputting the second situation data to the evaluation level and determining second evaluations indicating whether the second situation data satisfy predetermined second conditions, the determining of the second evaluations being influence

Abstract: An avatar apparatus that is adapted to represent a person and to process personal data of the person comprises an AI processor device that is adapted for machine learning of individual mental response characteristics of the person by way of data training, wherein the AI processor device is configured to generate semantic answers in response to questions in a manner dependent on the mental response characteristics, a first storage device that is coupled to the AI processor device and is adapted to store the mental response characteristics of the person, an input device that is coupled to the AI processor device and is adapted to interact with the person and/or to receive questions relating to the person, and an output device that is configured to output signals that represent the answers generated by the AI processor device. An avatar system with multiple avatar apparatuses, uses of the avatar apparatus, and an avatar method for operating the avatar apparatus, are also described.

Abstract: A method for processing search queries directed at a database containing medical sample data and/or samples from a multiplicity of samples of persons comprises the steps: entering a search query, with a request profile directed at retrieving at least one sample with sample data that have predetermined queried search parameters, into an AI processor device, searching for at least one selected sample using the AI processor device, the at least one selected sample satisfying the request profile at least with a specified probability, and outputting identification data of the at least one selected sample. A search platform apparatus that is configured to process search queries directed at a database containing medical sample data and/or samples from a multiplicity of samples of persons is also described.

Abstract: A sample collection kit for collecting plant samples, in particular algae samples for biomedical applications, comprises at least one sample container adapted to receive plant samples, a sensor arrangement having multiple sensors that can be used to capture sample data, a data recording device adapted to record the sample data, a tool arrangement having collection tools, which are adapted for harvesting the plant samples, and having processing tools, which are adapted for processing the plant samples, a container device adapted to receive the tool arrangement and the de-vice arrangement in a state that is isolated from the surroundings, and identification markings that include identification data and are attached to the at least one sample container, the collection tools and the processing tools, wherein there is provision for an authentication device that contains an electronically readable data carrier containing authentication data that represent a state of the sample collection kit, and the authentication

Abstract: A method for cryopreserving a plurality of cell clusters (1, 2) of biological cells includes the steps of fractionating the cell clusters (1, 2) into at least two fractions (4) in dependency on at least one property of the cell clusters (1, 2), collecting the fractions (4) in different containers (21), and cryopreserving the cell clusters (1, 2) of the at least two fractions (4), wherein specific pretreatment methods and/or freezing methods are used for each fraction. A cryopreservation apparatus (100), for cryopreserving a plurality of cell clusters (1, 2) of biological cells, having a fractionation device, is also described.

Abstract: The invention relates to a process for producing carrier particles for the cultivation of biological cells, comprising the steps of providing an aqueous suspension of hydrogel beads and freeze-drying of the hydrogel beads so that dry hydrogel particles are formed, wherein at least one lyoprotectant substance is added to the suspension, the hydrogel beads are loaded in the suspension with the lyoprotectant substance and the dry hydrogel particles receive a shape under the effect of the lyoprotectant substance, which shape approximates a spherical particle shape and is still maintained after rehydrating. The invention also relates to carrier particles for a cultivation of biological cells, which comprise dry hydrogel particles, preferably having a protein coating, and to applications of the carrier particles.

Abstract: The invention relates to a device for the temperature monitoring of a cryopreserved biological sample, comprising a sample container (in particular a cryotube) having a holding space for holding a biological sample and comprising at least one chamber, the interior of which is not fluidically connected to the holding space and is filled only partially with an indicator substance, the melting temperature of which lies in a range of ?20° C. to ?140° C. In particular, the chamber can be formed by a container that is detachably or pivotably fastened to the sample container. Alternatively, the chamber is formed by a double-walled slide-on part or the holding space of the sample container is double-walled, wherein an intermediate space between the inner wall and the outer wall is partially filled with the indicator substance.

Abstract: A method for processing genetic data, which comprise a series of sequence elements each representing a biomolecule, comprises the steps of forming sequence fragments (S2), wherein each sequence fragment comprises a section of the series of sequence elements having a fragment length of at least two sequence elements, applying a coding function to each of the sequence fragments in order to generate a multiplicity of encrypted fragment data items (S3) winch are each assigned to one of the sequence fragments, and storing the encrypted fragment data (S4), wherein the sequence fragments are formed in such a manner that the sections of the series of sequence elements overlap and each sequence element is included in at least two sequence fragments. A description is also given of a data processing device for processing genetic data and a method for querying a database containing encrypted fragment data which were generated and stored using the method for processing genetic data.

Abstract: The invention relates to a method for monitoring the temperature of a cryopreserved biological sample. The invention also relates to a device for monitoring the temperature of a cryopreserved biological sample. The device (10) for monitoring the temperature of a cryopreserved biological sample comprises a sample container (1) having a receiving space (2) for receiving a biological sample (6). The device also comprises at least one chamber (11) having an interior that is not fluidically connected to the receiving space (2) and is only partially filled with an indicator substance (7) with a melting temperature in a region of ?20° C. to ?140° C. The chamber (11) has a barrier (13) that causes the indicator substance (7) to move into a second sub-region (12b) of the chamber (11) when the indicator substance (7) in a first sub-region (12a) of the chamber is in the fluid aggregate state.

Abstract: Provided are methods in a system of at least a second and a third artificial intelligence unit, comprising: inputting first input values to at least one second artificial intelligence unit, and obtaining output values based on the input values from the at least one second artificial intelligence unit; at least temporarily storing situation data, the situation data comprising first input values and/or second output values of the at least one second unit; using the situation data as input values of the third artificial intelligence unit, the third artificial intelligence unit generating third output values in response to the input values; and checking whether the second output values of the at least one second unit satisfy one or more predetermined conditions based on the third output values. Also provided is a system for carrying out such a method.

Abstract: Provided is a method in a system of at least two artificial intelligence units, comprising inputting input values to at least a first artificial intelligence unit and a second artificial intelligence unit; obtaining first output values of the first artificial intelligence unit; forming one or more modulation functions based on the output values of the first artificial intelligence unit; applying the formed one or more modulation functions to one or more parameters of the second artificial intelligence unit, the one or more parameters influencing the processing of input values and the obtaining of output values in the second artificial intelligence unit; and finally obtaining second output values of the second artificial intelligence unit.

Abstract: The invention relates to a method for monitoring the temperature of a cryopreserved biological sample. The invention also relates to a device for monitoring the temperature of a cryopreserved biological sample. The device (10) for monitoring the temperature of a cryopreserved biological sample comprises a sample container (1) having a receiving space (2) for receiving a biological sample (6). The device also comprises at least one chamber (11) having an interior that is not fluidically connected to the receiving space (2) and is only partially filled with an indicator substance (7) with a melting temperature in a region of ?20° C. to ?140° C. The chamber (11) has a barrier (13) that causes the indicator substance (7) to move into a second sub-region (12b) of the chamber (11) when the indicator substance (7) in a first sub-region (12a) of the chamber is in the fluid aggregate state.

Abstract: The invention relates to a treatment device (100) which is designed for extracorporeal blood treatment of blood of a subject (2) for an immunotolerance enhancement of the subject (2), comprising at least one container (10) which has an interior (11) for receiving a blood sample (1) of blood of the subject (2) and an exchange device (20) designed to supply and/or discharge the blood sample (1) into or out of the interior (11) of the container (10) and comprising a cell exposure device (30) which is positioned in the container (10) and has biological cell material (31) with surface molecules, said cell material being arranged in the interior (11) of the container (10) for a material interaction with the blood sample (1), including a reaction of endogenous immune cells in the blood with the surface molecules of the cell material. The invention also relates to a kit for extracorporeal blood treatment and to a method for operating the treatment device.

Abstract: A sample holder device 100, 101 which is designed to hold biological samples 1 includes a base body 10 having at least one wall 11 which is arranged to delimit a sample receptacle 12, wherein the at least one wall 11 includes, at least on a surface facing the sample receptacle 12, a planar, carbon-based material which is impermeable to a liquid in sample receptacle 12, wherein the carbon-based material has such a high carbon content that the carbon-based material is opaque and electrically conductive. The sample holder device includes, e.g., a dish, in particular petri dish 101, a planar substrate, a multiwell plate, a sample beaker, in particular in the form of a beaker glass, a sample tube, in particular in the form of a test tube or a tube for cryopreservation (cryovial), and/or a hollow fiber. Methods for using the sample holder device are also described.

Abstract: The invention relates to a bioreactor (100) which is designed for cultivating biological cells (1), comprising a container (10) configured to receive a cultivation medium (2), and a plurality of substrate filaments (20) which are arranged in the container (10) and are configured for a temporary adherent coupling of the biological cells (1) to the substrate filaments (20). The substrate filaments (20) are provided with a surface layer (21) which is switchable between an adherence state, in which the biological cells (1) can be QI coupled adherently to the surface layer (21), and a release state, in which the adherent coupling of the biological cells (1) to the surface layer (21) is reduced in comparison to the binding state. The invention also relates to a method for processing biological cells (1) in the bioreactor (100).

Abstract: The invention relates to a device for the temperature monitoring of a cryopreserved biological sample, comprising a sample container having an accommodating space (2) for accommodating the sample and comprising an indicating apparatus, which can be arranged on the outside of the sample container, for monitoring at least one temperature limit value. The indicating apparatus has at least one cavity, which is only partially filled with an indicating substance, the melting temperature of which lies in a range from ?20 ° C. to ?140 ° C. The indicating apparatus can be designed, in particular, as a cylindrical body, which can be fastened to a cryotube as a bottom part, or alternatively as a double-walled hollow cylinder, which can be slid onto an outer lateral surface of the cryotube. The indicating apparatus can also be fastened to a lateral outer wall of the sample container, e.g. as a hollow body that can be inserted into a sleeve or insertion pocket.

Abstract: The invention relates to a method for producing three-dimensional, preferably porous, hydrogel structures by means of layer build-up technique, wherein the method comprises the following steps. Providing (S1) of a liquid hydrogel solution, preferably a liquid alginate solution, and a, preferably transportable, sample carrier. Layerwise applying (S2) the liquid hydrogel solution onto the sample carrier in a temperature environment, the temperature of which is below the freezing point of the hydrogel solution, to produce a frozen 3D layered hydrogel structure. In order to increase advantageously the porosity of the 3D layered hydrogel structure, i.e. the proportion of small voids, cavities and/or depressions in the 3D layered hydrogel structure, the method according to the invention further comprises the step of drying (S3) of the frozen 3D layered hydrogel structure, e.g. by means of freeze-drying, to produce a porous 3D hydrogel structure.