Patents by Inventor Helen R. Buckley

Helen R. Buckley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cytoplasmic antigens of candida albicans and methods of using the same
    Patent number: 4806465
    Abstract: Two novel hybridoma cell lines, ATCC #HB-8397 and ATCC #HB-8398 produce monoclonal antibody monospecific to a single determinant shared by a set of three closely related cytoplasmic antigens of Candida albicans. The antigens have molecular weights of 120-135 Kd, 48-52 Kd, and 35-38 Kd. The hybridomas are formed by fusing splenocytes from immunized BALB/c mice with SP2/O-Ag 14 myeloma cells. Monoclonal and monospecific, polyclonal antibodies to these cytoplasmic antigens find application in the immunodiagnosis of Candida infections.A procedure is provided for preparing partially purified cytoplasmic antigen of pathogenic Candida species for administration to splenocyte-donating mice. Also provided is a method for the biochemical purification of cytoplasmic antigen of a pathogenic Candida species used for the preparation of monoclonal and monospecific, polyclonal antisera thereto.
    Type: Grant
    Filed: March 30, 1987
    Date of Patent: February 21, 1989
    Assignee: Temple University of the Commonwealth System of Higher Education
    Inventors: Helen R. Buckley, Michael T. Largen, Nancy A. Strockbine
  • Monoclonal antibody to Candida albicans cytoplasmic antigens and methods of preparing same
    Patent number: 4670382
    Abstract: Two novel hybridoma cell lines, ATCC #HB-8397 and ATCC #HB-8398 produce monoclonal antibody monospecific to a single determinant shared by a set of three closely related cytoplasmic antigens of Candida albicans. The antigens have molecular weights of 120-135 Kd, 48-52 Kd, and 35-38 Kd. The hybridomas are formed by fusing splenocytes from immunized BALB/c mice with SP2/O-Ag 14 myeloma cells. Monoclonal and monospecific, polyclonal antibodies to these cytoplasmic antigens find application in the immunodiagnosis of Candida infections.A procedure is provided for preparing partially purified cytoplasmic antigen of pathogenic Candida species for administration to splenocyte-donating mice. Also provided is a method for the biochemical purification of cytoplasmic antigen of a pathogenic Candida species used for the preparation of monoclonal and monospecific, polyclonal antisera thereto.
    Type: Grant
    Filed: January 16, 1984
    Date of Patent: June 2, 1987
    Assignee: Temple University--of the Commonwealth System of Higher Education
    Inventors: Helen R. Buckley, Michael T. Largen, Nancy A. Strockbine