Abstract: The invention relates to a system for expressing nonribosomal peptide synthetases (NRPSs), polyketide synthases (PKS) or NRPS/PKS hybrid synth(et)ases. NRPS, PKS or hybrids thereof are large multi-domain proteins or multi-domain complexes, the expression of which for the production of peptides often causes difficulties. The invention correspondingly relates to a system for expressing portions of the enzymes which can be assembled post-translationally via protein-protein interactions, introduced in a targeted manner, to form multi-enzyme complexes. The invention discloses protein fragments of such an assembly, and the nucleic acids coding therefor. The invention also relates to a vector system for the protein fragments of the invention and its use for producing functional NRPS/PKS enzyme complexes.

Abstract: The present invention relates to a method of increasing the lipid content in an organism, in particular a micro algae by modulating the activity of a triacylglycerol (TAG) lipase. The invention also concerns organisms produced by this method, or microalgae, with increased lipid content, as well as their use in industry and medicine; in particular to obtain omega-3 unsaturated fatty acids. The invention relates furthermore to a nucleic acid from Phaeodactylum tricornutum, which encodes a new TAG lipase, and its use in influencing the proportion of fatty acids, in particular the content of polyunsaturated fatty acids in biomass.