Patents by Inventor Helle Fabricius Woldike
Helle Fabricius Woldike has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8535909Abstract: The present invention relates to a novel non-pathogenic Escherichia coli (E. coli) B BL21 strain comprising a deletion of group II capsular gene cluster, and the use thereof for the production of peptides.Type: GrantFiled: November 10, 2009Date of Patent: September 17, 2013Assignee: Novo Nordisk A/SInventors: Helle Fabricius Wöldike, Jianhui Deng, Xin Zhao, Yun Liu, Jing Su
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Publication number: 20110236929Abstract: The present invention relates to a novel non-pathogenic Escherichia coli (E. coli) B BL21 strain comprising a deletion of group II capsular gene cluster, and the use thereof for the production of peptides.Type: ApplicationFiled: November 10, 2009Publication date: September 29, 2011Applicant: Novo Nordisk A/SInventors: Helle Fabricius Wöldike, Jianhui Deng, Xin Zhao, Yun Liu, Jing Su
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Patent number: 7858747Abstract: Plasmid comprising a DNA tag encoding a peptide tag of the sequence MX1(X2X3)n wherein X1 represents K, R or H; X2 represents A, G, H, M, S or T; X3 represents K, R, H, A, Q or V; n represents an integer of 1 or larger; and wherein said DNA is operably-linked to a promoter sequence are provided.Type: GrantFiled: August 30, 2006Date of Patent: December 28, 2010Assignee: Novo Nordisk A/SInventors: Helle Fabricius Wöldike, Christine Bruun Schiødt
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Patent number: 7517668Abstract: A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.Type: GrantFiled: May 5, 1995Date of Patent: April 14, 2009Assignee: Novozymes A/SInventors: Esper Boel, Tove Christensen, Helle Fabricius Wöldike
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Patent number: 6171823Abstract: The invention relates to a method of producing an extracellular protein in a bacterium provided with an inner and an outer cell membrane, the method comprising: (a) providing a recombinant vector including a DNA construct comprising a DNA sequence encoding the prepropeptide or part of the prepropeptide of a bacterial extracellular protease selected from the group consisting of Achromobacter lyticus protease I, Bacillus metalloproteases and Bacillus serine proteases preceding and operably connected to a DNA sequence encoding a desired protein, (b) transforming cells of a microorganism provided with an inner and outer cell membrane with the recombinant vector of step (a), (c) culturing the transformed cells of step (b) under conditions permitting expression of said DNA insert and leakage of the bacterial extracellular protease propeptide fused to the desired protein into the culture medium, and (d) recovering the resulting protein from the medium.Type: GrantFiled: June 2, 1997Date of Patent: January 9, 2001Assignee: Novo Nordisk A/SInventors: Helle Fabricius Wöldike, Sven Hastrup
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Patent number: 5965384Abstract: A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.Type: GrantFiled: June 5, 1995Date of Patent: October 12, 1999Assignee: Novo Nordisk A/SInventors: Esper Boel, Tove Christensen, Helle Fabricius Woldike, Ida Birgitte Huge-Jensen
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Patent number: 5945328Abstract: Trypsin (trypsinogen) may be produced in a filamentous fungus by transforming a filamentous fungus with a vector comprising a DNA sequence encoding protrypsin or a derivative thereof N-terminally fused to a DNA sequence encoding a signal peptide, culturing the transformed filamentous fungus in a suitable culture medium to produce trypsinogen and recovering trypsinogen and/or trypsin from the medium.Type: GrantFiled: October 22, 1997Date of Patent: August 31, 1999Assignee: Novo Nordisk A/SInventors: Helle Fabricius Woldike, Thomas Borglum Kjeldsen
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Patent number: 5874558Abstract: A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.Type: GrantFiled: May 17, 1996Date of Patent: February 23, 1999Assignee: Novo NordiskInventors: Esper Boel, Tove Christensen, Helle Fabricius Woldike
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Patent number: 5766912Abstract: A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.Type: GrantFiled: April 20, 1994Date of Patent: June 16, 1998Assignee: Novo Nordisk A/SInventors: Esper Boel, Tove Christensen, Helle Fabricius Woldike, Ida Birgitte Huge-Jensen
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Patent number: 5763254Abstract: The present invention relates to isolated and purified cellulose- or hemicellulose-degrading enzymes, comprising a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalytically active domain and the carbohydrate binding domain, wherein the carbohydrate binding domain has the following core sequence: ##STR1## wherein the enzyme is endogenous to a strain of a fungus other than Trichoderma or Phanerochaete.Type: GrantFiled: June 7, 1995Date of Patent: June 9, 1998Assignee: Novo Nordisk A/SInventors: Helle Fabricius Woldike, Fred Hagen, Carsten Mailand Hjort, Sven Hastrup
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Patent number: 5686593Abstract: The present invention relates to cellulose- or hemicellulose-degrading enzymes which is derivable from a fungus other than Trichoderma or Phanerochaete, and which comprises a carbohydrate binding domain homologous to a terminal A region of Trichoderma reesei cellulases, which carbohydrate binding domain comprises the following amino acid sequence. ##STR1## or a subsequence thereof capable of effecting binding of the enzyme to an insoluble cellulosic or hemicellulosic substrate.Type: GrantFiled: June 7, 1995Date of Patent: November 11, 1997Assignee: Novo Nordisk A/SInventors: Helle Fabricius Woldike, Fred Hagen, Carsten Mailand Hjort, Sven Hastrup