Abstract: Method for operating a Laser Scanning Microscope, in which a probe is illuminated by at least one scanner and a picture recording takes place with a temperature measurement taking place in the scanner and/or in the scanner driver and only on reaching a threshold temperature a cooling device is started and advantageously on switching on of the cooling device the picture recording is interrupted or on reaching a threshold temperature a display device actuates an optical or an acoustic display.

Abstract: A Laser Scanning Microscope, preferably with line-shaped sampling, whereby illumination radiation from the Microscope is guided over a sample with at least one galvanometer scanner. The scanner has a mechanical deflection limit. A means for the determination of a current increase is provided in the scanner. On reaching a threshold value, the operating voltage of the scanner is switched off until it declines below the threshold value and preferably an optical and/or acoustic display device is provided, which displays the switching on and off of the scanner.

Abstract: A method and arrangement are disclosed for increasing the depth contrast in microscope imaging. The method and implementation described can be designated as structured illumination for generating quasi-confocal optical sections. In implementing the method, a grating structure located in the field diaphragm plane of a microscope, the object plane and the TV intermediate image plane of a microscope are arranged confocally. The term “confocally” refers to the fact that the grating, object and the intermediate image plane are positioned on optically conjugate planes. By this arrangement, the grating structure is projected in the object plane of the microscope and the object which is structured in this way is imaged in the TV intermediate image plane of the microscope by the optical system following it. Optical sections are generated by calculating the modulation depth of the structured object.

Abstract: The invention relates to a method for increasing the depth of contrast during microscopic imaging. The method and implementation described can be designated as structured illumination, which creates quasi-confocal optical sections. During the implementation of the method, a grid structure, located in the illumination field stop plane of a microscope, the object plane and the TV intermediate-image plane of a microscope are arranged “confocally”. This assembly enables the grid structure to be projected into the object plane of the microscope and the object, thus structured, to be reproduced on the TV intermediate-image plane using the following optical system. Optical sections are created by the calculation of the modulation depth of the structured object. The object is reproduced perpendicular to the direction of observation on several focus planes, to achieve a 3-dimensional registration of said object and is detected using an array-detector (e.g. CCD camera).