Abstract: The current invention comprises a method for producing a heterologous polypeptide with a reduced degree of fucose modification in a mammalian cell by cultivating the mammalian cell under conditions suitable for the expression of said heterologous polypeptide, and recovering the heterologous polypeptide from the mammalian cell or the culture, wherein in said mammalian cell the enzymatic activity of ?1,6-fucosyltransferase is reduced by means of an shRNA directed against ?1,6-fucosyltransferase mRNA.

Abstract: The current invention comprises a method for producing a heterologous polypeptide with a reduced degree of fucose modification in a mammalian cell by cultivating the mammalian cell under conditions suitable for the expression of said heterologous polypeptide, and recovering the heterologous polypeptide from the mammalian cell or the culture, wherein in said mammalian cell the enzymatic activity of ?1,6-fucosyltransferase is reduced by means of an shRNA directed against ?1,6-fucosyltransferase mRNA.

Abstract: The current invention comprises a method for the selection of a mammalian cell by transfecting a mammalian cell with a nucleic acid comprising a part of a nucleic acid encoding a polypeptide that catalyzes an ?1,6-glycosidic bond formation between fucose and an asparagine-linked N-acetylglucosamine and cultivating the transfected mammalian cell in the presence of Lens culinaris agglutinin (LCA) and selecting a mammalian cell viable under these conditions.

Abstract: The present application relates to a method for modulating the formation and/or maintenance of adipocyte tissue by ectopically contacting adipocyte cells, especially adipocyte stem/progenitor cells, in vitro or in vivo, with a hedgehog therapeutic or ptc therapeutic in an amount effective to alter the growth state the treated cells, e.g., relative to the absence of administeration of the hedgehog therapeutic or ptc therapeutic.

Abstract: A polypeptide with the properties of collagenase class I from Clostridium histolyticum (CHC I) and the amino acid sequence SEQ ID NO:2 which is optionally N-terminally extended by one or several amino acids of the sequence SEQ ID NO:3 is advantageously suitable for the isolation of cells from mammalian tissue and human tissue.

Abstract: The invention concerns a DNA coding a eukaryotic highly active alkaline phosphatase with a specific activity of more than 3000 U/mg. The invention also concerns a process for the production of a DNA according to the invention, a vector containing the DNA according to the invention and a cell line containing this vector. Furthermore the invention concerns a recombinant highly active alkaline phosphatase with a specific activity of more than 3000 U/mg which is coded by the DNA according to the invention.

Abstract: Process for disintegrating cell tissue and releasing cells or groups of cells contained therein by incubating the cell tissue with a recombinant neutral protease from Clostridium histolyticum which is coded bya) a DNA of nucleotides 1027-1965 from SEQ ID NO:3 or a DNA which is complementary thereto,b) nucleic acids which hybridize with a DNA of nucleotides 1027-1965 from SEQ ID NO:3,c) nucleic acids which, without the degeneracy of the genetic code, would hybridize with one of the nucleic acids mentioned in a) or b)and is the product of a prokaryotic or eukaryotic expression of an exogenous nucleic acid, until the cells or groups of cells have been released to the desired extent and separating the cells or groups of cells from the cell tissue fractions.

Abstract: A recombinant protein with D-hydantoinase activity which has the amino acid sequence SEQ ID NO 1 is obtainable in large amounts and has an improved temperature stability.

Abstract: A recombinant protein with D-hydantoinase activity which has the amino acid sequence SEQ ID NO 1 is obtainable in large amounts and has an improved temperature stability.

Abstract: In a process for the production of recombinant, biologically active, eukaryotic alkaline phosphatase a DNA sequence coding for a eukaryotic alkaline phosphatase is expressed in a prokaryotic host cell and the expression product is obtained in an active form by cell lysis, solubilization under denaturing conditions and subsequent renaturation. In this process the renaturation step is carried out in the presence of one or several stabilizing agents.

Abstract: The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has1.) the nucleic acid sequence shown in FIG. (1),2.) a sequence corresponding to it within the scope of the degeneracy of the genetic code or(3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions.Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity.

Abstract: The invention concerns a recombinant DNA which contains(1) the sequence shown in SEQ ID NO: 1,(2) a sequence corresponding to this sequence within the scope of the degeneracy of the genetic code or(3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent hybridizing conditionswhereby the DNA sequence codes for a protein with N-carbamoyl sarcosine amidohydrolase activity.Furthermore the invention concerns a recombinant vector with the DNA according to the present invention as well as a process for the isolation of a recombinant protein with N-carbamoyl sarcosine amidohydrolase activity and its use for the determination of creatinine.

Abstract: The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has(1) the nucleic acid sequence shown in FIG. (1),(2) a sequence corresponding to it within the scope of the degeneracy of the genetic code or(3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions.Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity.