Abstract: Device for the continuous electrophoresis in a carrier free buffer current including a separating chamber, in which a separating space is located between two plane parallel glass plates held in a two-part frame and is separated from electrode chambers by permeable membranes. A dividing joint is provided between two frame-parts at least partially at an angle to a dividing plane of the frame located between and parallel to the glass plates. The membranes, when the frame-parts are pressed together along the dividing joint, are pressed against the surfaces of the frame-parts which form a part of the dividing joint, with a force component lying in the direction of the dividing plane, thus forming a seal. The separating chamber permits easy assembly and disassembly thereof.