Patents by Inventor Helmut Wurst

Helmut Wurst has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11065364
    Abstract: A combination comprising, spatially separate from one another, a first component and a second component, where the first component comprises crosslinkable albumin and the second component comprises a polymer, wherein non-terminal monomer units of the polymer comprise at least partially, more particularly only partially, an albumin-crosslinking group. Additionally disclosed is a reaction product obtainable by means of the combination, to a medical device, to a medicinal product for innovative therapies, to a kit, to a discharge apparatus, and to a functionalized hyaluronic acid.
    Type: Grant
    Filed: August 28, 2017
    Date of Patent: July 20, 2021
    Assignee: TETEC Tissue Engineering Technologies AG
    Inventors: Helmut Wurst, Nils Clausen, Nina Baldassi
  • Patent number: 10500154
    Abstract: The invention relates to an injectable biocompatible composition based on a polymeric support as well as to a method for producing it, which composition which comprises at least one hydrophilic polymer, wherein the polymer is polymerizable in situ to form a gel, and wherein the hydrophilic polymer is crosslinkable serum albumin or crosslinkable serum protein. The composition can be used in the restoration, the reconstruction, and/or the replacement of tissues and/or organs, or as a drug release implant in mammals. The composition is particularly suitable for treating cartilage disorders of a diseased or injured articular site in a mammal.
    Type: Grant
    Filed: July 27, 2010
    Date of Patent: December 10, 2019
    Assignee: NMI NATURWISSENSCHAFTLICHES UND MEDIZINISCHES INSTITUT AN DER UNIVERSITAET TUEBINGEN
    Inventors: Juergen Mollenhauer, Karin Benz, Barbara Platz, Helmut Wurst, Reinout Stoop
  • Publication number: 20190367869
    Abstract: A 3D culture system based on chemically defined hydrogel scaffolds for the cultivation and expansion of pancreas-type organoids from isolated pancreatic cells in vitro.
    Type: Application
    Filed: May 30, 2019
    Publication date: December 5, 2019
    Inventors: Brigitte ANGRES, Nadine RISCHERT, Helmut WURST
  • Publication number: 20190192733
    Abstract: A combination comprising, spatially separate from one another, a first component and a second component, where the first component comprises crosslinkable albumin and the second component comprises a polymer, wherein non-terminal monomer units of the polymer comprise at least partially, more particularly only partially, an albumin-crosslinking group. Additionally disclosed is a reaction product obtainable by means of the combination, to a medical device, to a medicinal product for innovative therapies, to a kit, to a discharge apparatus, and to a functionalized hyaluronic acid.
    Type: Application
    Filed: August 28, 2017
    Publication date: June 27, 2019
    Inventors: Helmut WURST, Nils CLAUSEN, Nina BALDASSI
  • Patent number: 10196602
    Abstract: The present invention relates to a biomaterial for cell or tissue culture, based on a polymeric carrier, which contains at least one crosslinkable hydrophilic polymer. The polymer is functionalized with groups that are selected from maleimide, vinylsulfonic, acrylate, alkyl halide, azirine, pyridyl, thionitrobenzene acid groups, or arylating groups. The invention relates further to a method of production of said biomaterial, and the use of particular functionalizing groups for the production of a biomaterial for the cultivation of tissue and/or cells. The biomaterial can have biofactors that exert a particular action on cells.
    Type: Grant
    Filed: January 11, 2010
    Date of Patent: February 5, 2019
    Assignee: NMI NATURWISSENSCHAFTLICHES UND MEDIZINISCHES INSTITUT AN DER UNIVERSITAET TUEBINGEN
    Inventor: Helmut Wurst
  • Publication number: 20180311376
    Abstract: The invention relates to medical compositions, a medical hydrogel, a medical kit and a multichamber discharge device for use in the prevention and/or treatment of a disease of the facet joints and/or in the replacement and/or regeneration of articular facets.
    Type: Application
    Filed: October 20, 2016
    Publication date: November 1, 2018
    Inventors: Helmut Wurst, Christoph Gaissmaier, Nils Clausen
  • Patent number: 8952095
    Abstract: A peptide cross-linking agent in the form of a linear molecule has a molecular mass of 3 to approximately 60 kDa. The peptide cross-linking agents are used for cross-linking functionalized polymers to form hydrogels having two or more components.
    Type: Grant
    Filed: February 4, 2011
    Date of Patent: February 10, 2015
    Assignee: CELLENDES GmbH
    Inventors: Helmut Wurst, Karima Larbi, Markus Herrmann
  • Publication number: 20150038681
    Abstract: A method of preparing a product, preferably in the form of a functionalized high molecular weight component such as, for example, a functionalized protein includes passing a first liquid comprising a component A along one side of a semi-permeable membrane and passing a second liquid comprising a component B along another, preferably opposing, side of the membrane, wherein the membrane excludes passage of component A but allows passage of component B, and after passage of component B through the membrane, a chemical reaction takes place between component A and component B, preferably with the formation of covalent bonds.
    Type: Application
    Filed: August 4, 2014
    Publication date: February 5, 2015
    Inventors: Juergen Mollenhauer, Heinz W. Joseph, Helmut Wurst
  • Publication number: 20130052736
    Abstract: A peptide cross-linking agent in the form of a linear molecule has a molecular mass of 3 to approximately 60 kDa. The peptide cross-linking agents are used for cross-linking functionalized polymers to form hydrogels having two or more components.
    Type: Application
    Filed: February 4, 2011
    Publication date: February 28, 2013
    Applicant: CELLENDES GmbH
    Inventors: Helmut Wurst, Karima Larbi, Markus Herrmann
  • Publication number: 20100322993
    Abstract: The invention relates to an injectable biocompatible composition based on a polymeric support as well as to a method for producing it, which composition which comprises at least one hydrophilic polymer, wherein the polymer is polymerizable in situ to form a gel, and wherein the hydrophilic polymer is crosslinkable serum albumin or crosslinkable serum protein. The composition can be used in the restoration, the reconstruction, and/or the replacement of tissues and/or organs, or as a drug release implant in mammals. The composition is particularly suitable for treating cartilage disorders of a diseased or injured articular site in a mammal.
    Type: Application
    Filed: July 27, 2010
    Publication date: December 23, 2010
    Inventors: Juergen Mollenhauer, Karin Benz, Barbara Platz, Helmut Wurst, Reinout Stoop
  • Publication number: 20100184223
    Abstract: The present invention relates to a biomaterial for cell or tissue culture, based on a polymeric carrier, which contains at least one crosslinkable hydrophilic polymer. The polymer is functionalized with groups that are selected from maleimide, vinylsulfonic, acrylate, alkyl halide, azirine, pyridyl, thionitrobenzene acid groups, or arylating groups. The invention relates further to a method of production of said biomaterial, and the use of particular functionalizing groups for the production of a biomaterial for the cultivation of tissue and/or cells. The biomaterial can have biofactors that exert a particular action on cells.
    Type: Application
    Filed: January 11, 2010
    Publication date: July 22, 2010
    Inventor: Helmut Wurst
  • Publication number: 20020173016
    Abstract: High throughput lyophilized polymerase devices and methods for their use in the production of nucleic acids using template dependent polymerase reactions are provided. The subject devices are typically made up of a multi-well substrate that includes in a least one well a lyophilized nucleic acid polymerase composition. The subject nucleic acid polymerase compositions include at least one polymerase and a carbohydrate stabilizing composition that is made up of at least one low molecular weight sugar and a starch. In many embodiments, the compositions also include buffer components and nucleotides, as well as a temperature dependent polymerase inhibitor, e.g., a polymerase specific antibody. Also provided are kits that include the subject devices.
    Type: Application
    Filed: March 27, 2001
    Publication date: November 21, 2002
    Inventors: Helmut Wurst, Ningyue Zhao
  • Publication number: 20020119465
    Abstract: Enzyme compositions, kits comprising the same and methods for their use in one-step RT-PCR are provided. The subject enzyme compositions at least include a mutant thermostable DNA polymerase and a mutant reverse transcriptase. In preferred embodiments, the mutant thermostable DNA polymerase is an N-terminal deletion mutant of Taq polymerase and the mutant reverse transcriptase is a point mutation mutant of MMLV-RT. The subject kits, in addition to the above described mutant thermostable DNA polymerase and mutant reverse transcriptase, at least include one of, and usually both of, dNTPs and a buffer composition, where the subject kits may further include additional reagents, including nucleic acids, a thermostabilizing agent, a glycine based osmolyte and the like. In practicing the subject methods, a reaction mix that at least includes template RNA, the above described mutant polymerase and reverse transcriptase, dNTPs, buffer, and nucleic acid primers is prepared.
    Type: Application
    Filed: August 16, 2001
    Publication date: August 29, 2002
    Inventors: Ningyue Zhao, Helmut Wurst
  • Patent number: 6383755
    Abstract: Methods are provided for evaluating the fidelity of a given set of polymerase chain reaction conditions. In the subject methods, a template polydeoxyribonucleotide is amplified under the to be evaluated polymerase chain reaction conditions, where the template polydeoxyribonucleotide includes a pseudo restriction endonuclease restriction site. The resultant amplified product population is then contacted with the corresponding restriction endonuclease and resultant cleavage products, if any, are detected. The fidelity of the polymerase chain reaction conditions is then derived from the detected cleavage products (or absence thereof). Also provided are kits for use in practicing the subject methods. The subject methods are suited for determining the fidelity of a given polymerase under PCR conditions, and are particularly suited for determining the fidelity of a thermostable polymerase under PCR conditions.
    Type: Grant
    Filed: September 6, 2000
    Date of Patent: May 7, 2002
    Assignee: Clontech Laboratories, Inc.
    Inventors: Gregory D. Davis, Helmut Wurst
  • Patent number: 6300073
    Abstract: Enzyme compositions, kits comprising the same and methods for their use in one-step RT-PCR are provided. The subject enzyme compositions at least include a mutant thermostable DNA polymerase and a mutant reverse transcriptase. In preferred embodiments, the mutant thermostable DNA polymerase is an N-terminal deletion mutant of Taq polymerase and the mutant reverse transcriptase is a point mutation mutant of MMLV-RT. The subject kits, in addition to the above described mutant thermostable DNA polymerase and mutant reverse transcriptase, at least include one of, and usually both of, dNTPs and a buffer composition, where the subject kits may further include additional reagents, including nucleic acids, a thermostabilizing agent, a glycine based osmolyte and the like. In practicing the subject methods, a reaction mix that at least includes template RNA, the above described mutant polymerase and reverse transcriptase, dNTPs, buffer, and nucleic acid primers is prepared.
    Type: Grant
    Filed: October 1, 1999
    Date of Patent: October 9, 2001
    Assignee: Clontech Laboratories, Inc.
    Inventors: Ningyue Zhao, Helmut Wurst
  • Patent number: 6130045
    Abstract: A thermostable enzyme having polymerase activity and substantially no nuclease activity is provided. The thermostable enzyme is characterized by comprising a sequence of nine amino acid residues at least proximal to the N-terminus that has less than 50% but greater than 40% amino acid sequence identity with residues 280 to 288 of the naturally occurring Thermus aquaticus polymerase. The subject enzyme finds use in a variety of different application in which polynucleotides are enzymatically produced, particularly in PCR based applications.
    Type: Grant
    Filed: June 11, 1998
    Date of Patent: October 10, 2000
    Assignee: Clontech Laboratories, Inc.
    Inventors: Helmut Wurst, Zhi-Hao Qiu
  • Patent number: 5976842
    Abstract: Methods and compositions are provided for performing high fidelity polymerase chain reactions. In the subject methods, primer extension products are produced with a low error frequency rate through the use of at least one of unequal concentrations of dNTPs or a melting point reducing agent. Also provided are kits and reagent mixtures for use in the subject methods. The subject invention finds use in a variety of applications, particularly in applications where high fidelity PCR is desired.
    Type: Grant
    Filed: October 30, 1997
    Date of Patent: November 2, 1999
    Assignee: Clontech Laboratories, Inc.
    Inventor: Helmut Wurst