Patents by Inventor Heloisa Ribeiro Tunes de Sousa

Heloisa Ribeiro Tunes de Sousa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
    Patent number: 6699692
    Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.
    Type: Grant
    Filed: February 29, 2000
    Date of Patent: March 2, 2004
    Assignees: Biomm S.A., Universidad de Brasilia
    Inventors: Spartaco Astolfi Filho, Beatriz Dolabela de Lima, Josef Ernst Thiemann, Heloisa Ribeiro Tunes de Sousa, Luciano Vilela
  • Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
    Publication number: 20030082742
    Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.
    Type: Application
    Filed: February 29, 2000
    Publication date: May 1, 2003
    Inventors: Spartaco F. Astolfi, Beatriz Dolabela de Lima, Josef Ernst Thiemann, Heloisa Ribeiro Tunes de Sousa, Luciano Vilela
  • Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
    Patent number: 6509452
    Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.
    Type: Grant
    Filed: May 7, 1999
    Date of Patent: January 21, 2003
    Assignees: BIOMM S.A., Universidad de Brasilia
    Inventors: Spartaco Astolfi Filho, Beatriz Dolabela de Lima, Josef Ernst Thiemann, Heloisa Ribeiro Tunes de Sousa, Luciano Vilela
  • Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
    Patent number: 6281329
    Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.
    Type: Grant
    Filed: May 7, 1999
    Date of Patent: August 28, 2001
    Assignees: Biobrás S.A., Universidade de Brasilia
    Inventors: Spartaco Astolfi Filho, Beatriz Dolabela de Lima, Josef Ernst Thiemann, Heloisa Ribeiro Tunes de Sousa, Luciano Vilela
  • Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
    Patent number: 6068993
    Abstract: The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag.
    Type: Grant
    Filed: July 2, 1997
    Date of Patent: May 30, 2000
    Assignees: Biobras SA, Universidade de Brasilia
    Inventors: Spartaco Astolfi Filho, Beatriz Dolabela de Lima, Josef Ernst Thiemann, Heloisa Ribeiro Tunes de Sousa, Luciano Vilela