Abstract: Present invention relates to a method to determine the genotype of organisms by RAPD analysis and more specifically, to establish the relatedness of individual organisms across and within species. RAPD uses genotypic information of an organism to give an organism specific DNA fragment of different sizes. The present invention provide methods and a set of oligonucleotide primers for performing amplification and other enzymatic reactions on nucleic acid molecules that have been collected directly as environmental DNA or DNA derived form pure isolates. More specifically, the present invention relates to a novel method of genetic analysis using a set of sub-sequence, which occurs as inverted repeats in different genome with different frequencies. All bacterial cultures used in this study have been isolated from activated biomass collected from effluent treatment plants. The bacteria have been sub-cultured repeatedly to obtain pure cultures. All plating has been carried out on Luria Broth plates with 2% agar.

Abstract: The present invention relates to a method for detecting the presence of water born pathogens and indicator microorganism including bacteria from water sample by selecting the target gene carried in template DNA by amplifying the target DNA using specific primers with biotinylated tag consist of all or a substantial part of 5?-CTGATCGAATGGCTGCCAGGCTCC-3? and 5?-CAACCAGACGATAGTTATCACGCA-3? and taq DNA polymerase to get desired biotinylated tagged probe followed by hybridization of biotinylated tagged probe with target gene in template DNA followed by enzyme coupled reaction.