Abstract: The disclosed embodiments concern methods for determining sequences of interest using targeted unique molecular index (TUMI) sequences that are uniquely associated with individual polynucleotide fragments in plants, such as those present in a transgenic event, a site-specific mutation or a wild type variant. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

Abstract: Liquid compositions for preserving and storing pollen, especially maize pollen, include pollen suspensions in solutions with particular pH ranges. Methods for preserving and storing pollen, especially maize pollen, include suspending pollen in solutions with particular pH ranges and storing them at particular temperatures.

Abstract: The disclosed embodiments concern methods for determining sequences of interest using targeted unique molecular index (TUMI) sequences that are uniquely associated with individual polynucleotide fragments in plants, such as those present in a transgenic event, a site-specific mutation or a wild type variant. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

Abstract: The present invention provides in vitro methods for culturing cereal endosperm that allow for the development of the endosperm in a manner that is analogous to endosperm development in planta. The methods involve isolating fertilized embryo sacs and exposing the embryo sac or portion thereof to a plant culture medium for an extended period of time so as to promote the growth and development of the endosperm therein or the growth and development of both the endosperm and embryo therein. The invention further provides methods for introducing polypeptides and polynucleotides into the cultured fertilized embryo sacs or portions thereof.