Abstract: The present invention relates to a nucleic acid nanostructure comprising a first surface and a second surface, wherein said first surface and said second surface are located at opposing sides of said nanostructure, wherein said first surface comprises at least a first targeting agent and said second surface comprises at least a second targeting agent and at least a third targeting agent. The present invention further relates to a composition comprising a nucleic acid nanostructure. The invention also relates to a nanostructure and a composition for use in medicine, and to a nanostructure and a composition for use in a method of preventing or treating a disease. Furthermore, the present invention relates to a method of preparing a nanostructure and to a use of a nanostructure for binding first target and the second target.

Abstract: The present invention relates to a nucleic acid nanostructure comprising one or more, preferably at least two, targeting agent(s) and a plurality of dye molecules, preferably at least to dye molecules. The present invention further relates to a composition, preferably pharmaceutical composition, comprising a nucleic acid nanostructure. The present invention also relates to a nanostructure or composition for use in medicine, preferably for use in medical imaging, and for use in a method of preventing, treating, and/or diagnosing a disease. Furthermore, the present invention relates to a method of labelling a target in a sample, to the use of a nanostructure as a stain, and to a kit. Moreover, the present invention further relates to a method of preparing a nanostructure.

Abstract: The present invention relates to conditionally switchable nanostructures, and systems comprising the same. In particular, the present invention pertains to a method for linking the identification of at least one molecule to a conformational reconfiguration of a nanostructure. The invention is further based on the finding that coupling molecules, such as antibodies or antigen binding fragments, can stabilize subunit interactions of nanostructures at otherwise unfavourable conditions. Additionally, the present invention also relates to a system comprising the nanostructures of this invention as well as methods for utilizing the nanostructures or the system of this invention. Finally, substances and/or compositions comprising at least one or a plurality of nanostructures for use as a medicament, or for use in a diagnostic method, are also provided.

Abstract: IN The present invention relates to a method for the recombinant production of DNA single stranded molecules, comprising the steps of (1) providing a pseudogene nucleic acid; (2) integrating the pseudogene nucleic acid into a vector, transforming bacterial cells with said vector and producing a precursor ssDNA from said vector under bacterial culture conditions; (3) isolating the precursor ssDNA from the bacterial culture; (4) digesting the precursor ss DNA under reaction conditions where self-cleaving DNA sequences become active; and (5) separating and obtaining the target single stranded DNA oligo- or polynucleotide(s). The method of the present invention is suitable for the mass production of DNA single stranded molecules. The present invention further relates to the use of the target single stranded DNA oligo- or polynucleotide(s), in particular in DNA nanotechnology, or as research tools.

Abstract: Systems comprising a translation module, an amplification module comprising one or more sensing modalities, and a detection module configured to accept the amplifier output and methods of using the same are provided. The systems or methods can be capable of detecting trace amounts of nucleic acid molecules with high sensitivity and specificity in a device-free system with naked-eye readable output. The systems can comprise three layers incorporated into a lateral flow assay and/or a paper strip assay, in such systems, the first layer can comprise the translation module, the second layer can comprise the amplification module, and the third layer can comprise the detection module.

Abstract: Systems and methods for determining the presence or absence of an analyte including a nucleic acid (e.g., DNA and RNA), a small molecule (e.g., proteins and amino acid chains), and one or more electrolytes (e.g., Na+ and K+). The system or method may detect multiple analytes (e.g., a first DNA and a second DNA) and/or multiple types of analytes (e.g., an RNA and an antibody protein). The signal readout provided by the system or method may be readily understood and may be correlated with a health condition (e.g., hydration or exposure to an infectious agent). The system may be wearable and may analyze one or more biofluids.

Abstract: The present invention relates to a macromolecule-based nanostructure, such as a DNA-based nanostructure, for encapsulating a virus or viral particle, to a composition comprising a virus or viral particle encapsulated by such a macromolecule-based nanostructure according to the present invention, and to a method for encapsulating a virus or viral particle by using such a macromolecule-based nanostructure

Abstract: The present invention relates to a conditionally switchable nanostructure configured to assume a first configuration and a second configuration, wherein the nanostructure comprises a binding site that is configured to bind to a binding target, and wherein the accessibility of the binding site for the binding target in the second configuration is different to the accessibility of the binding site for the binding target in the first configuration. Further, the nanostructure is configured to assume the first configuration when none of the coupling sites of the coupling site set is coupled to its respective coupling target and to assume the second configuration when each coupling site of a subset of the coupling site set is coupled to its respective coupling target, wherein the subset comprises at least one coupling site. Additionally, the present technology also relates to a system comprising the nanostructure as well as a method utilizing the nanostructure or the system.

Abstract: Systems and indicators for determining the presence or absence of specific environmental, exposure, or biological conditions are provided. Indicators include a plurality of sensors, each sensor independently having a biological or chemical sensing modality to detect one or more analytes of interest. Analytes of interest include nucleic acids (e.g., DNA, RNA, etc.), proteins, peptides, and other amino acid chains and may come from a subject or the microbiome of a subject. The signals from the plurality of sensors may be processed to provide a readily understandable readout concerning a health condition or predisposition of a subject, such as cancer and exposure to coronavirus. The signals from the plurality of sensors may be colorimetric (e.g. a color change in response to the presence or absence of an analyte), and a plurality of colorimetric signals may be combined to provide a readily understandable colorimetric output. Indicators may be wearable.

Abstract: The present invention relates to a novel method for stabilizing nucleic acid nanostructures by curing with ultraviolet light, particularly by crosslinking pyrimidine nucleotides.

Abstract: The present invention relates to a method for the recombinant production of DNA single stranded molecules, comprising the steps of (1) providing a pseudogene nucleic acid; (2) integrating the pseudogene nucleic acid into a vector, transforming bacterial cells with said vector and producing a precursor ssDNA from said vector under bacterial culture conditions; (3) isolating the precursor ssDNA from the bacterial culture; (4) digesting the precursor ssDNA under reaction conditions where self-cleaving DNA sequences become active; and (5) separating and obtaining the target single stranded DNA oligo- or polynucleotide(s). The method of the present invention is suitable for the mass production of DNA single stranded molecules. The present invention further relates to the use of the target single stranded DNA oligo- or polynucleotide(s), in particular in DNA nanotechnology, or as research tools.

Abstract: Techniques for controlling properties of nucleic acid nanostructures include receiving data that indicates a sequence of nucleotides on at least a first strand of a nucleic acid. Values are determined for at least one physical property for each portion of the at least first strand. Based at least in part on a numerical model and the physical properties for each portion, a value is determined of at least one derived property of a nanostructure that comprises the at least first strand of nucleic acid. In some embodiments, information gained from the numerical model is used iteratively in order to optimize or improve one or more of the properties of the target DNA origami structure.

Abstract: Techniques for controlling properties of nucleic acid nanostructures include receiving data that indicates a sequence of nucleotides on at least a first strand of a nucleic acid. Values are determined for at least one physical property for each portion of the at least first strand. Based at least in part on a numerical model and the physical properties for each portion, a value is determined of at least one derived property of a nanostructure that comprises the at least first strand of nucleic acid. In some embodiments, information gained from the numerical model is used iteratively in order to optimize or improve one or more of the properties of the target DNA origami structure.