Abstract: The invention relates to detection the presence of a target molecule in a sample, wherein the sample is contacted with a substrate, the substrate subsequently being washed in a wash step. In particular, the invention relates to a method of detecting the presence of a target molecule in a sample, the method comprising: (a) contacting the sample (37) with a substrate having immobilized thereon probe molecules that specifically binds to the target molecule; (b) washing the substrate (38) in a wash step by a wash fluid in order to remove or dilute unbound target molecules; (c) detect the presence of resultant binding complexes (39) on the substrate to determine whether the target molecule is present in the sample. The wash fluid being substantially refractive index matched to the substrate.

Abstract: A non-leaching adhesive system and its use in a liquid immersion objective for immersion-writing of masters for optical discs are disclosed. The adhesive system comprises at least one monomer, selected from among the group of acrylate and methacrylate monomers, allylic monomers, norbornene monomers, hybrid monomers thereof, containing chemically different polymerizable groups, and multifunctional thiol monomers, provided that said thiol is used in combination with at least one of said non-thiol monomers; and a polymerization initiator. At least one of said monomers, not being a thiol, is provided with at least two functional polymerizable groups to obtain a crosslinked polymer network. The polymerization initiator is preferably an initiator that can be activated both thermally and with UV radiation. The adhesive system may further contain a reactive diluent. Further the use of the present adhesive system in mounting a liquid immersion objective is disclosed.

Abstract: The present invention provide a qualitative or quantitative luminescence sensor, for example a bio sensor or chemical sensor, using sub-wavelength aperture or slit structures, i.e. using apertures or slit structures having a smallest dimension smaller than the wavelength of the excitation radiation in the medium that fills the aperture or slit structure. The invention furthermore provides a method for the detection of luminescence radiation generated by one or more luminophores present in aperture or slit structure in such a luminescence sensor.

Abstract: A non-leaching adhesive system and its use in a liquid immersion objective for immersion-writing of masters for optical discs are disclosed. The adhesive system comprises at least one monomer, selected from among the group of acrylate and methacrylate monomers, allylic monomers, norbornene monomers, hybrid monomers thereof, containing chemically different polymeriazble groups, and multifunctional thiol monomers, provided that said thiol is used in combination with at least one of said non-thiol monomers; and a polymerization initiator. At least one of said monomers, not being a thiol, is provided with at least two functional polymerizable groups to obtain a crosslinked polymer network. The polymerization initiator is preferably an initiator that can be activated both thermally and with UV radiation. The adhesive system may further contain a reactive diluent. Further the use of the present adhesive system in mounting a liquid immersion objective is disclosed.

Abstract: The invention relates to a microelectronic sensor device and a method for the investigation of biological target substances (20), for example oligonucleotides like DNA fragments. In one embodiment, the device comprises a reaction surface (RS) to which target specific reactants (10) are attached and which lies between a sample chamber (SC) and an array of selectively controllable heating elements (HE). The temperature profile in the sample chamber (SC) can be controlled as desired to provide for example conditions for a PCR and/or for a controlled melting of hybridizations. The reactant (10) and/or the target substance (20) comprises a label (12) with an observable property, like fluorescence, that changes if the target substance (20) is bound to the reactant (10), said property being detected by an array of sensor elements, for example photosensors (SE).

Abstract: The present invention relates to a method of determining the concentration of an analyte in a sample and/or the binding kinetics of an analyte to an analyte sensor molecule. For this purpose the invention relies on detecting the interaction between the analyte and the analyte sensor molecule with the latter being physically adsorbed to a solid, porous support which is in the form of a micro array. In a preferred embodiment, the method may be operated in repeated cycles.

Abstract: This invention provides a biosensor solid or hydrogel substrate comprising one or more temperature indicating agents, each of said one or more temperature indicating agents operating by changing its optical properties and being deposited in and/or at the surface of the biosensor solid or hydrogel substrate as one or more layers and/or spots

Abstract: The invention relates to a device for analyzing one or more samples for the presence, amount or identity of one or more target molecules in the samples, comprising one or more capture sites whereby the device comprises a gas evolving means. The gas evolved by the gas evolving means moves unbound target molecules away from the capture site and therefore helps to increase the efficacy of the analysis.

Abstract: The invention relates to a printer device for producing biological test arrays by depositing an array of biofluids onto a substrate. The invention further relates to the use of such a device in the production of biological test arrays. The invention also relates to a method for producing a biological test array. The invention moreover relates to a biological test array. The method according to the invention is failure-proof, and results in biological test arrays of superior quality.

Abstract: A biosensor device (10) for detecting of molecules of an analyte in a sample (23) comprising: an identification element (18) comprising at least one self assembling monolayer (26) having a first surface, a transducer element (20) comprising a metal electrode (34) for receiving an electric signal from the reaction of the molecules in the sample with the at least one self assembling monolayer (26), and at least one electronic element (14, 16) for receiving the electric signal from the transducer element (20), for processing, and/or storing the electric signal. The at least one self-assembling monolayer (26) comprises at least one carboxylic acid-group for coupling the at least one self-assembling monolayer (26) to the surface (32) of the metal electrode (34).

Abstract: The present invention concerns a lens system. More specifically, it concerns a lens system comprising a first lens (3), a deflection element (5) and a second lens (6), wherein the deflection element (5) is arranged between the first lens (3) and the second lens (6). The deflection element comprises at least a first annular zone and a second annular zone, the annular zones being arranged in a concentric fashion and wherein the deflection angle of each annular zone is different from the deflection angle of every other annular zone. Furthermore, the present invention concerns a temperature analysis system comprising a lens system and the use of a temperature analysis system.

Abstract: The invention provides an ink jet device for releasing a plurality of substances onto a substrate, the device comprising at least one print head, which is positionable relative to the substrate, and which comprises at least one nozzle, provided to eject a droplet. The ink jet device is designed such that the plurality of substances can be deposited onto the substrate in a predefined pattern in a limited amount of moves of the at least one print head relative to the substrate.

Abstract: The invention relates to a biosensor device with an actuating element comprising a biorecognition element that is capable of binding target molecules from a sample of interest, wherein the actuating element comprises a polymer material, can be actuated by actuating means between a first and a second position.

Abstract: The invention provides an ink jet device for releasing controllably a plurality of substances onto a substrate, the device comprising at least a print head comprising a nozzle, the device comprising at least a transducer provided to eject a droplet out of the nozzle, wherein a detection means is assigned to the ink jet device such that the substances are discriminable from each other by means of the detection of the behaviour of the transducer.

Abstract: The present invention relates to a luminescence sensor (10), such as a luminescence biosensor or a luminescence chemical sensor, comprising at least one aperture (1) having a first lateral dimension larger than the diffraction limit of excitation radiation (3) in a medium the at least one aperture (1) is filled with and a second lateral dimension below the diffraction limit of excitation radiation (3) in a medium the at least one aperture (1) is filled with. The invention furthermore relates to a method for the detection of luminescence radiation (5), e.g. fluorescence radiation, generated by at least one optically variable molecule (2), e.g. fluorophore, in the at least one aperture (1). The excitation radiation (3) is preferably polarised to suppress the excitation radiation (3) in the apertures (1). The luminescence sensor (10) according to the present invention is able to detect relatively low concentrations of optically variable molecules (2), e.g. fluorophores.

Abstract: A method is provided that prepares resuspendable particles of a compound. The particles are prepared by drying a liquid that comprises the compound. The particles are dried in such a way that they do not aggregate on redispersion. The particles that are thus obtained are especially suitable for use in bio sensors, as contrast agent or as part of a pharmaceutical composition.

Abstract: The present invention relates to a camera arrangement as for instance used in a mobile phone that utilizes a liquid crystal based lens for providing an adjustable depth of focus. The camera arrangement comprises a photo sensor (201) array and at least two lenses (202, 203, 204) that are arranged in a fixed and unitary arrangement. At least one of the lenses (202) comprises a liquid crystal layer (101) that provides for adjustable focal length in that lens. The additional lens(-es) (203, 204) might have fixed or adjustable focal length depending on the application. According to one embodiment, the camera arrangement comprises at least one additional adjustable lens ant the lenses are arranged so as to provide for adjustable depth? of focus as well as for an adjustable depth of field.

Abstract: A sensor is described for use with at least one optical detector (5), the sensor comprising a substrate (11) with optical outlets, a porous membrane (12) and micro fluidic channels (13) for conducting analyte fluid towards sensing locations (10) of the porous membrane (12). The sensing locations (10) are adapted for at least restraining light variable molecules (23) which bind to analytes to be determined. The optical output of the light variable molecules changes when they are in close proximity to a target molecule. The micro fluidic channels (13) are shaped to reflect light emitted from the sensing locations (10) towards the optical outlets and the substrate has diffracting optical elements (15) aligned with microfluidic channels to diffract light towards the optical outlets. The diffracting optical elements can be lenses.

Abstract: The invention relates to a substrate material for analyzing fluids which is elastic and allows radial lateral movement of sample within the substrate material upon application of pressure.

Abstract: The present invention provides micro-fluidic systems, a method for the manufacturing of such a micro-fluidic system and a method for controlling or manipulating a fluid flow through micro-channels of a such a micro-fluidic system. Herefore, an inner side of a wall of a microchannel is provided with actuator elements which can change shape and orientation as a response to an external stimulus. Through this change of shape and orientation the flow of a fluid through a microchannel may be controlled and manipulated.